Experiment: RCH2 grown on a colony of 8R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1871 and Psest_1872 are separated by 22 nucleotides Psest_1872 and Psest_1873 are separated by 3 nucleotides
Psest_1871: Psest_1871 - Predicted esterase of the alpha-beta hydrolase superfamily, at 1,981,183 to 1,983,414
_1871
Psest_1872: Psest_1872 - selT/selW/selH selenoprotein domain, at 1,983,437 to 1,983,718
_1872
Psest_1873: Psest_1873 - Predicted permease, DMT superfamily, at 1,983,722 to 1,984,600
_1873
Position (kb)
1983
1984 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1982.607 kb on + strand, within Psest_1871 at 1983.069 kb on + strand, within Psest_1871 at 1983.399 kb on + strand at 1983.400 kb on - strand at 1983.402 kb on + strand at 1983.402 kb on + strand at 1983.403 kb on - strand at 1983.404 kb on + strand at 1983.404 kb on + strand at 1983.404 kb on + strand at 1983.404 kb on + strand at 1983.405 kb on - strand at 1983.405 kb on - strand at 1983.470 kb on + strand, within Psest_1872 at 1983.471 kb on - strand, within Psest_1872 at 1983.471 kb on - strand, within Psest_1872 at 1983.471 kb on - strand, within Psest_1872 at 1983.556 kb on - strand, within Psest_1872 at 1983.556 kb on - strand, within Psest_1872 at 1983.589 kb on + strand, within Psest_1872 at 1983.884 kb on + strand, within Psest_1873 at 1983.884 kb on + strand, within Psest_1873 at 1983.885 kb on - strand, within Psest_1873 at 1983.885 kb on - strand, within Psest_1873 at 1983.885 kb on - strand, within Psest_1873 at 1983.885 kb on - strand, within Psest_1873 at 1984.329 kb on - strand, within Psest_1873 at 1984.701 kb on + strand at 1984.701 kb on + strand at 1984.701 kb on + strand at 1984.702 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of 8R2A remove 1,982,607 + Psest_1871 0.64 +1.7 1,983,069 + Psest_1871 0.84 -1.2 1,983,399 + -0.2 1,983,400 - -3.7 1,983,402 + -1.4 1,983,402 + +1.3 1,983,403 - +2.2 1,983,404 + +0.8 1,983,404 + -0.4 1,983,404 + -2.3 1,983,404 + +0.1 1,983,405 - -0.3 1,983,405 - +1.6 1,983,470 + Psest_1872 0.12 -1.8 1,983,471 - Psest_1872 0.12 -1.1 1,983,471 - Psest_1872 0.12 -1.5 1,983,471 - Psest_1872 0.12 -0.8 1,983,556 - Psest_1872 0.42 -1.8 1,983,556 - Psest_1872 0.42 -2.8 1,983,589 + Psest_1872 0.54 -0.2 1,983,884 + Psest_1873 0.18 +2.8 1,983,884 + Psest_1873 0.18 -0.2 1,983,885 - Psest_1873 0.19 -2.4 1,983,885 - Psest_1873 0.19 -2.9 1,983,885 - Psest_1873 0.19 -0.4 1,983,885 - Psest_1873 0.19 -0.4 1,984,329 - Psest_1873 0.69 -0.7 1,984,701 + +2.0 1,984,701 + -2.4 1,984,701 + -0.4 1,984,702 - +0.4
Or see this region's nucleotide sequence