Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0273

Experiment: RCH2 grown on a colony of N2F3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0272 and Psest_0273 are separated by 94 nucleotidesPsest_0273 and Psest_0274 are separated by 133 nucleotidesPsest_0274 and Psest_0275 overlap by 4 nucleotidesPsest_0275 and Psest_0276 are separated by 9 nucleotides Psest_0272: Psest_0272 - arginine deiminase, at 288,144 to 289,397 _0272 Psest_0273: Psest_0273 - arginine/ornithine antiporter, at 289,492 to 290,919 _0273 Psest_0274: Psest_0274 - hypothetical protein, at 291,053 to 291,466 _0274 Psest_0275: Psest_0275 - Protein of unknown function (DUF3392)., at 291,463 to 291,786 _0275 Psest_0276: Psest_0276 - putative oxygen-independent coproporphyrinogen III oxidase, at 291,796 to 292,929 _0276 Position (kb) 289 290 291Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 288.814 kb on + strand, within Psest_0272at 288.814 kb on + strand, within Psest_0272at 288.887 kb on - strand, within Psest_0272at 288.887 kb on - strand, within Psest_0272at 288.887 kb on - strand, within Psest_0272at 288.961 kb on + strand, within Psest_0272at 289.080 kb on + strand, within Psest_0272at 289.080 kb on + strand, within Psest_0272at 289.080 kb on + strand, within Psest_0272at 289.080 kb on + strand, within Psest_0272at 289.080 kb on + strand, within Psest_0272at 289.080 kb on + strand, within Psest_0272at 289.080 kb on + strand, within Psest_0272at 289.081 kb on - strand, within Psest_0272at 289.081 kb on - strand, within Psest_0272at 289.081 kb on - strand, within Psest_0272at 289.081 kb on - strand, within Psest_0272at 289.081 kb on - strand, within Psest_0272at 289.081 kb on - strand, within Psest_0272at 289.081 kb on - strand, within Psest_0272at 289.081 kb on - strand, within Psest_0272at 289.341 kb on + strandat 289.341 kb on + strandat 289.341 kb on + strandat 289.341 kb on + strandat 289.341 kb on + strandat 289.402 kb on + strandat 289.402 kb on + strandat 289.403 kb on - strandat 289.403 kb on - strandat 289.403 kb on - strandat 289.474 kb on + strandat 289.514 kb on + strandat 289.514 kb on + strandat 289.515 kb on - strandat 289.515 kb on - strandat 289.515 kb on - strandat 289.543 kb on + strandat 289.544 kb on - strandat 289.640 kb on + strand, within Psest_0273at 289.640 kb on + strand, within Psest_0273at 289.640 kb on + strand, within Psest_0273at 289.660 kb on + strand, within Psest_0273at 289.660 kb on + strand, within Psest_0273at 289.660 kb on + strand, within Psest_0273at 289.660 kb on + strand, within Psest_0273at 289.660 kb on + strand, within Psest_0273at 289.660 kb on + strand, within Psest_0273at 289.661 kb on - strand, within Psest_0273at 289.661 kb on - strand, within Psest_0273at 289.661 kb on - strand, within Psest_0273at 289.661 kb on - strand, within Psest_0273at 289.661 kb on - strand, within Psest_0273at 289.664 kb on + strand, within Psest_0273at 289.664 kb on + strand, within Psest_0273at 289.664 kb on + strand, within Psest_0273at 289.665 kb on - strand, within Psest_0273at 289.665 kb on - strand, within Psest_0273at 289.665 kb on - strand, within Psest_0273at 289.665 kb on - strand, within Psest_0273at 289.665 kb on - strand, within Psest_0273at 289.665 kb on - strand, within Psest_0273at 289.665 kb on - strand, within Psest_0273at 289.682 kb on + strand, within Psest_0273at 289.683 kb on - strand, within Psest_0273at 289.700 kb on + strand, within Psest_0273at 289.701 kb on - strand, within Psest_0273at 289.761 kb on - strand, within Psest_0273at 289.811 kb on + strand, within Psest_0273at 289.812 kb on - strand, within Psest_0273at 289.841 kb on + strand, within Psest_0273at 289.841 kb on + strand, within Psest_0273at 289.856 kb on + strand, within Psest_0273at 289.856 kb on + strand, within Psest_0273at 289.856 kb on + strand, within Psest_0273at 290.290 kb on + strand, within Psest_0273at 290.342 kb on - strand, within Psest_0273at 290.342 kb on - strand, within Psest_0273at 290.577 kb on - strand, within Psest_0273at 290.577 kb on - strand, within Psest_0273at 290.606 kb on + strand, within Psest_0273at 290.607 kb on - strand, within Psest_0273at 290.607 kb on - strand, within Psest_0273at 290.607 kb on - strand, within Psest_0273at 290.607 kb on - strand, within Psest_0273at 290.664 kb on + strand, within Psest_0273at 290.664 kb on + strand, within Psest_0273at 290.665 kb on - strand, within Psest_0273at 290.666 kb on + strand, within Psest_0273at 290.666 kb on + strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.667 kb on - strand, within Psest_0273at 290.697 kb on - strand, within Psest_0273at 290.891 kb on - strandat 290.891 kb on - strandat 290.891 kb on - strandat 290.891 kb on - strandat 290.891 kb on - strandat 290.924 kb on - strandat 290.955 kb on + strandat 290.955 kb on + strandat 290.979 kb on + strandat 290.980 kb on - strandat 290.980 kb on - strandat 291.023 kb on + strandat 291.024 kb on - strandat 291.123 kb on + strand, within Psest_0274at 291.123 kb on + strand, within Psest_0274at 291.123 kb on + strand, within Psest_0274at 291.123 kb on + strand, within Psest_0274at 291.124 kb on - strand, within Psest_0274at 291.124 kb on - strand, within Psest_0274at 291.124 kb on - strand, within Psest_0274at 291.212 kb on + strand, within Psest_0274at 291.212 kb on + strand, within Psest_0274at 291.212 kb on + strand, within Psest_0274at 291.213 kb on - strand, within Psest_0274at 291.222 kb on + strand, within Psest_0274at 291.243 kb on + strand, within Psest_0274at 291.243 kb on + strand, within Psest_0274at 291.243 kb on + strand, within Psest_0274at 291.244 kb on - strand, within Psest_0274at 291.244 kb on - strand, within Psest_0274at 291.244 kb on - strand, within Psest_0274at 291.246 kb on + strand, within Psest_0274at 291.246 kb on + strand, within Psest_0274at 291.246 kb on + strand, within Psest_0274at 291.246 kb on + strand, within Psest_0274at 291.246 kb on + strand, within Psest_0274at 291.247 kb on - strand, within Psest_0274at 291.247 kb on - strand, within Psest_0274at 291.247 kb on - strand, within Psest_0274at 291.247 kb on - strand, within Psest_0274at 291.247 kb on - strand, within Psest_0274at 291.247 kb on - strand, within Psest_0274at 291.247 kb on - strand, within Psest_0274at 291.247 kb on - strand, within Psest_0274at 291.247 kb on - strand, within Psest_0274at 291.249 kb on + strand, within Psest_0274at 291.250 kb on - strand, within Psest_0274at 291.250 kb on - strand, within Psest_0274at 291.464 kb on + strandat 291.464 kb on + strandat 291.465 kb on - strandat 291.527 kb on + strand, within Psest_0275at 291.527 kb on + strand, within Psest_0275at 291.527 kb on + strand, within Psest_0275at 291.527 kb on + strand, within Psest_0275at 291.528 kb on - strand, within Psest_0275at 291.528 kb on - strand, within Psest_0275at 291.528 kb on - strand, within Psest_0275at 291.528 kb on - strand, within Psest_0275at 291.528 kb on - strand, within Psest_0275at 291.723 kb on + strand, within Psest_0275at 291.775 kb on + strandat 291.797 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3
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288,814 + Psest_0272 0.53 +1.5
288,814 + Psest_0272 0.53 -0.7
288,887 - Psest_0272 0.59 -0.7
288,887 - Psest_0272 0.59 +0.3
288,887 - Psest_0272 0.59 -0.2
288,961 + Psest_0272 0.65 +1.1
289,080 + Psest_0272 0.75 -2.2
289,080 + Psest_0272 0.75 -1.1
289,080 + Psest_0272 0.75 -3.1
289,080 + Psest_0272 0.75 -0.7
289,080 + Psest_0272 0.75 -2.1
289,080 + Psest_0272 0.75 -0.9
289,080 + Psest_0272 0.75 -0.4
289,081 - Psest_0272 0.75 -0.9
289,081 - Psest_0272 0.75 -2.7
289,081 - Psest_0272 0.75 -1.2
289,081 - Psest_0272 0.75 +0.8
289,081 - Psest_0272 0.75 -1.4
289,081 - Psest_0272 0.75 -0.9
289,081 - Psest_0272 0.75 -0.3
289,081 - Psest_0272 0.75 +2.1
289,341 + -0.6
289,341 + -1.1
289,341 + -2.5
289,341 + +1.2
289,341 + +1.0
289,402 + -1.1
289,402 + +2.2
289,403 - -1.2
289,403 - +1.4
289,403 - -0.7
289,474 + +1.9
289,514 + +1.2
289,514 + -1.2
289,515 - +2.6
289,515 - -2.7
289,515 - +0.3
289,543 + +0.5
289,544 - -0.3
289,640 + Psest_0273 0.10 -2.8
289,640 + Psest_0273 0.10 -3.6
289,640 + Psest_0273 0.10 -0.5
289,660 + Psest_0273 0.12 -1.4
289,660 + Psest_0273 0.12 -1.5
289,660 + Psest_0273 0.12 +0.6
289,660 + Psest_0273 0.12 -2.8
289,660 + Psest_0273 0.12 +0.6
289,660 + Psest_0273 0.12 -0.7
289,661 - Psest_0273 0.12 +0.1
289,661 - Psest_0273 0.12 +0.4
289,661 - Psest_0273 0.12 -0.2
289,661 - Psest_0273 0.12 +1.3
289,661 - Psest_0273 0.12 -0.2
289,664 + Psest_0273 0.12 +2.7
289,664 + Psest_0273 0.12 -0.9
289,664 + Psest_0273 0.12 +1.2
289,665 - Psest_0273 0.12 +2.6
289,665 - Psest_0273 0.12 -0.2
289,665 - Psest_0273 0.12 -2.2
289,665 - Psest_0273 0.12 -2.2
289,665 - Psest_0273 0.12 -0.7
289,665 - Psest_0273 0.12 -1.4
289,665 - Psest_0273 0.12 +0.1
289,682 + Psest_0273 0.13 +0.8
289,683 - Psest_0273 0.13 -1.0
289,700 + Psest_0273 0.15 -2.5
289,701 - Psest_0273 0.15 -2.3
289,761 - Psest_0273 0.19 -2.6
289,811 + Psest_0273 0.22 +0.6
289,812 - Psest_0273 0.22 -1.5
289,841 + Psest_0273 0.24 -0.2
289,841 + Psest_0273 0.24 +2.6
289,856 + Psest_0273 0.25 +0.4
289,856 + Psest_0273 0.25 +0.3
289,856 + Psest_0273 0.25 +1.1
290,290 + Psest_0273 0.56 +1.2
290,342 - Psest_0273 0.60 -1.5
290,342 - Psest_0273 0.60 -0.0
290,577 - Psest_0273 0.76 +0.5
290,577 - Psest_0273 0.76 +0.4
290,606 + Psest_0273 0.78 +0.1
290,607 - Psest_0273 0.78 -2.2
290,607 - Psest_0273 0.78 +1.8
290,607 - Psest_0273 0.78 +0.5
290,607 - Psest_0273 0.78 -0.9
290,664 + Psest_0273 0.82 +1.3
290,664 + Psest_0273 0.82 +0.1
290,665 - Psest_0273 0.82 -1.6
290,666 + Psest_0273 0.82 -3.3
290,666 + Psest_0273 0.82 -1.4
290,667 - Psest_0273 0.82 +1.5
290,667 - Psest_0273 0.82 -1.1
290,667 - Psest_0273 0.82 -1.9
290,667 - Psest_0273 0.82 +1.2
290,667 - Psest_0273 0.82 +1.1
290,667 - Psest_0273 0.82 +1.1
290,667 - Psest_0273 0.82 -0.9
290,667 - Psest_0273 0.82 +2.1
290,667 - Psest_0273 0.82 -0.7
290,667 - Psest_0273 0.82 +0.5
290,697 - Psest_0273 0.84 +0.1
290,891 - -0.1
290,891 - -1.2
290,891 - -2.4
290,891 - -1.1
290,891 - +0.3
290,924 - +0.7
290,955 + -3.5
290,955 + +0.6
290,979 + +1.0
290,980 - +1.6
290,980 - -0.4
291,023 + +0.8
291,024 - -1.4
291,123 + Psest_0274 0.17 +0.7
291,123 + Psest_0274 0.17 -0.1
291,123 + Psest_0274 0.17 +2.6
291,123 + Psest_0274 0.17 -1.6
291,124 - Psest_0274 0.17 +3.4
291,124 - Psest_0274 0.17 -0.9
291,124 - Psest_0274 0.17 +0.7
291,212 + Psest_0274 0.38 -2.8
291,212 + Psest_0274 0.38 +0.9
291,212 + Psest_0274 0.38 -2.4
291,213 - Psest_0274 0.39 -1.2
291,222 + Psest_0274 0.41 -1.5
291,243 + Psest_0274 0.46 +0.8
291,243 + Psest_0274 0.46 +2.0
291,243 + Psest_0274 0.46 +0.3
291,244 - Psest_0274 0.46 +1.1
291,244 - Psest_0274 0.46 +0.0
291,244 - Psest_0274 0.46 -1.9
291,246 + Psest_0274 0.47 -1.3
291,246 + Psest_0274 0.47 -1.9
291,246 + Psest_0274 0.47 +1.1
291,246 + Psest_0274 0.47 -1.6
291,246 + Psest_0274 0.47 -0.4
291,247 - Psest_0274 0.47 -2.4
291,247 - Psest_0274 0.47 +0.7
291,247 - Psest_0274 0.47 -1.5
291,247 - Psest_0274 0.47 -2.4
291,247 - Psest_0274 0.47 -1.0
291,247 - Psest_0274 0.47 +0.8
291,247 - Psest_0274 0.47 +3.1
291,247 - Psest_0274 0.47 -0.2
291,247 - Psest_0274 0.47 +0.4
291,249 + Psest_0274 0.47 +2.1
291,250 - Psest_0274 0.48 +0.1
291,250 - Psest_0274 0.48 -0.5
291,464 + -0.1
291,464 + +1.7
291,465 - +1.3
291,527 + Psest_0275 0.20 +0.4
291,527 + Psest_0275 0.20 -1.7
291,527 + Psest_0275 0.20 -1.1
291,527 + Psest_0275 0.20 -2.2
291,528 - Psest_0275 0.20 -2.3
291,528 - Psest_0275 0.20 -1.8
291,528 - Psest_0275 0.20 +0.3
291,528 - Psest_0275 0.20 +2.1
291,528 - Psest_0275 0.20 -1.4
291,723 + Psest_0275 0.80 -0.2
291,775 + -0.1
291,797 + +2.1

Or see this region's nucleotide sequence