Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0851 and Psest_0852 are separated by 171 nucleotides Psest_0852 and Psest_0853 overlap by 14 nucleotides Psest_0853 and Psest_0854 are separated by 18 nucleotides
Psest_0851: Psest_0851 - Transcriptional regulators, at 921,490 to 922,359
_0851
Psest_0852: Psest_0852 - glucose-6-phosphate 1-dehydrogenase, at 922,531 to 924,000
_0852
Psest_0853: Psest_0853 - 6-phosphogluconolactonase, at 923,987 to 924,700
_0853
Psest_0854: Psest_0854 - Entner-Doudoroff aldolase, at 924,719 to 925,456
_0854
Position (kb) 922
923
924
925 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 921.542 kb on - strand at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.647 kb on + strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.648 kb on - strand, within Psest_0851 at 921.797 kb on + strand, within Psest_0851 at 922.067 kb on + strand, within Psest_0851 at 922.067 kb on + strand, within Psest_0851 at 922.068 kb on - strand, within Psest_0851 at 922.068 kb on - strand, within Psest_0851 at 922.348 kb on + strand at 922.399 kb on + strand at 922.399 kb on + strand at 922.399 kb on + strand at 922.542 kb on - strand at 922.542 kb on - strand at 922.618 kb on + strand at 922.619 kb on - strand at 922.793 kb on - strand, within Psest_0852 at 922.897 kb on + strand, within Psest_0852 at 923.041 kb on + strand, within Psest_0852 at 923.041 kb on + strand, within Psest_0852 at 923.056 kb on + strand, within Psest_0852 at 923.387 kb on - strand, within Psest_0852 at 923.426 kb on - strand, within Psest_0852 at 923.524 kb on + strand, within Psest_0852 at 923.524 kb on + strand, within Psest_0852 at 923.524 kb on + strand, within Psest_0852 at 923.596 kb on + strand, within Psest_0852 at 923.596 kb on + strand, within Psest_0852 at 923.597 kb on - strand, within Psest_0852 at 923.758 kb on + strand, within Psest_0852 at 923.759 kb on - strand, within Psest_0852 at 923.855 kb on - strand at 923.920 kb on + strand at 923.986 kb on + strand at 923.986 kb on + strand at 923.998 kb on + strand at 924.321 kb on - strand, within Psest_0853 at 924.370 kb on - strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.396 kb on + strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.397 kb on - strand, within Psest_0853 at 924.686 kb on + strand at 924.687 kb on - strand at 924.687 kb on - strand at 924.727 kb on + strand at 924.728 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 921,542 - -1.1 921,647 + Psest_0851 0.18 +1.1 921,647 + Psest_0851 0.18 +0.2 921,647 + Psest_0851 0.18 -0.4 921,647 + Psest_0851 0.18 -1.6 921,647 + Psest_0851 0.18 -0.6 921,647 + Psest_0851 0.18 -2.5 921,647 + Psest_0851 0.18 -0.4 921,647 + Psest_0851 0.18 -2.1 921,647 + Psest_0851 0.18 -2.9 921,647 + Psest_0851 0.18 -1.1 921,647 + Psest_0851 0.18 +2.0 921,647 + Psest_0851 0.18 -1.6 921,647 + Psest_0851 0.18 -3.4 921,647 + Psest_0851 0.18 -3.7 921,647 + Psest_0851 0.18 -3.2 921,647 + Psest_0851 0.18 -1.7 921,647 + Psest_0851 0.18 -1.1 921,647 + Psest_0851 0.18 -1.4 921,647 + Psest_0851 0.18 +0.8 921,647 + Psest_0851 0.18 -1.3 921,647 + Psest_0851 0.18 -1.9 921,647 + Psest_0851 0.18 -0.4 921,648 - Psest_0851 0.18 +0.1 921,648 - Psest_0851 0.18 -0.8 921,648 - Psest_0851 0.18 -0.8 921,648 - Psest_0851 0.18 -0.7 921,648 - Psest_0851 0.18 -2.4 921,648 - Psest_0851 0.18 -3.7 921,648 - Psest_0851 0.18 -1.1 921,648 - Psest_0851 0.18 -3.8 921,648 - Psest_0851 0.18 -2.5 921,648 - Psest_0851 0.18 -1.0 921,648 - Psest_0851 0.18 -0.3 921,648 - Psest_0851 0.18 -0.8 921,648 - Psest_0851 0.18 -1.6 921,648 - Psest_0851 0.18 -2.3 921,648 - Psest_0851 0.18 -3.3 921,648 - Psest_0851 0.18 -2.6 921,648 - Psest_0851 0.18 -2.3 921,648 - Psest_0851 0.18 -1.7 921,648 - Psest_0851 0.18 -1.0 921,648 - Psest_0851 0.18 -3.4 921,797 + Psest_0851 0.35 -0.9 922,067 + Psest_0851 0.66 -0.4 922,067 + Psest_0851 0.66 -1.8 922,068 - Psest_0851 0.66 -1.4 922,068 - Psest_0851 0.66 -4.2 922,348 + -2.2 922,399 + -0.8 922,399 + -2.7 922,399 + +0.3 922,542 - -3.1 922,542 - +0.6 922,618 + +2.2 922,619 - -0.4 922,793 - Psest_0852 0.18 -2.7 922,897 + Psest_0852 0.25 -3.5 923,041 + Psest_0852 0.35 -2.3 923,041 + Psest_0852 0.35 -2.0 923,056 + Psest_0852 0.36 -1.3 923,387 - Psest_0852 0.58 -0.4 923,426 - Psest_0852 0.61 -2.4 923,524 + Psest_0852 0.68 +2.2 923,524 + Psest_0852 0.68 -2.5 923,524 + Psest_0852 0.68 +1.1 923,596 + Psest_0852 0.72 -0.1 923,596 + Psest_0852 0.72 -3.8 923,597 - Psest_0852 0.73 -2.7 923,758 + Psest_0852 0.83 -0.0 923,759 - Psest_0852 0.84 -1.9 923,855 - -1.1 923,920 + +0.2 923,986 + -0.2 923,986 + +0.9 923,998 + -0.8 924,321 - Psest_0853 0.47 +1.1 924,370 - Psest_0853 0.54 +0.7 924,396 + Psest_0853 0.57 -1.4 924,396 + Psest_0853 0.57 -1.3 924,396 + Psest_0853 0.57 -0.0 924,396 + Psest_0853 0.57 -1.7 924,396 + Psest_0853 0.57 +1.4 924,397 - Psest_0853 0.57 +1.4 924,397 - Psest_0853 0.57 +1.2 924,397 - Psest_0853 0.57 -0.1 924,397 - Psest_0853 0.57 -2.8 924,397 - Psest_0853 0.57 +0.7 924,686 + +1.6 924,687 - -0.5 924,687 - +2.1 924,727 + -0.7 924,728 - +0.8
Or see this region's nucleotide sequence