Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4151 and Psest_4152 overlap by 1 nucleotides Psest_4152 and Psest_4153 overlap by 1 nucleotides Psest_4153 and Psest_4154 are separated by 98 nucleotides Psest_4154 and Psest_4155 are separated by 172 nucleotides
Psest_4151: Psest_4151 - Flagellar hook capping protein, at 4,334,409 to 4,335,074
_4151
Psest_4152: Psest_4152 - flagellar basal-body rod protein FlgC, at 4,335,074 to 4,335,502
_4152
Psest_4153: Psest_4153 - flagellar basal-body rod protein FlgB, at 4,335,502 to 4,335,867
_4153
Psest_4154: Psest_4154 - flagella basal body P-ring formation protein FlgA, at 4,335,966 to 4,336,754
_4154
Psest_4155: Psest_4155 - Flagellin and related hook-associated proteins, at 4,336,927 to 4,337,763
_4155
Position (kb)
4335
4336
4337 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4335.068 kb on + strand at 4335.207 kb on + strand, within Psest_4152 at 4335.207 kb on + strand, within Psest_4152 at 4335.207 kb on + strand, within Psest_4152 at 4335.207 kb on + strand, within Psest_4152 at 4335.207 kb on + strand, within Psest_4152 at 4335.207 kb on + strand, within Psest_4152 at 4335.208 kb on - strand, within Psest_4152 at 4335.213 kb on + strand, within Psest_4152 at 4335.213 kb on + strand, within Psest_4152 at 4335.214 kb on - strand, within Psest_4152 at 4335.288 kb on - strand, within Psest_4152 at 4335.346 kb on - strand, within Psest_4152 at 4335.483 kb on + strand at 4335.483 kb on + strand at 4335.483 kb on + strand at 4335.484 kb on - strand at 4335.484 kb on - strand at 4335.859 kb on + strand at 4335.860 kb on - strand at 4335.861 kb on + strand at 4335.861 kb on + strand at 4335.861 kb on + strand at 4335.862 kb on - strand at 4335.862 kb on - strand at 4335.946 kb on + strand at 4335.946 kb on + strand at 4335.947 kb on - strand at 4336.049 kb on + strand, within Psest_4154 at 4336.049 kb on + strand, within Psest_4154 at 4336.049 kb on + strand, within Psest_4154 at 4336.049 kb on + strand, within Psest_4154 at 4336.049 kb on + strand, within Psest_4154 at 4336.050 kb on - strand, within Psest_4154 at 4336.476 kb on + strand, within Psest_4154 at 4336.476 kb on + strand, within Psest_4154 at 4336.476 kb on + strand, within Psest_4154 at 4336.476 kb on + strand, within Psest_4154 at 4336.477 kb on - strand, within Psest_4154 at 4336.690 kb on + strand at 4336.690 kb on + strand at 4336.690 kb on + strand at 4336.690 kb on + strand at 4336.691 kb on - strand at 4336.746 kb on - strand at 4336.752 kb on + strand at 4336.752 kb on + strand at 4336.752 kb on + strand at 4336.752 kb on + strand at 4336.753 kb on - strand at 4336.841 kb on - strand at 4336.898 kb on - strand at 4336.908 kb on + strand at 4336.908 kb on + strand at 4336.908 kb on + strand at 4336.908 kb on + strand at 4336.909 kb on - strand at 4336.909 kb on - strand at 4336.951 kb on + strand at 4336.952 kb on - strand at 4336.952 kb on - strand at 4336.952 kb on - strand at 4336.952 kb on - strand at 4336.952 kb on - strand at 4336.952 kb on - strand at 4337.238 kb on + strand, within Psest_4155 at 4337.320 kb on + strand, within Psest_4155 at 4337.339 kb on + strand, within Psest_4155 at 4337.340 kb on - strand, within Psest_4155 at 4337.340 kb on - strand, within Psest_4155 at 4337.385 kb on + strand, within Psest_4155 at 4337.385 kb on + strand, within Psest_4155 at 4337.385 kb on + strand, within Psest_4155 at 4337.385 kb on + strand, within Psest_4155 at 4337.386 kb on - strand, within Psest_4155 at 4337.386 kb on - strand, within Psest_4155 at 4337.386 kb on - strand, within Psest_4155 at 4337.413 kb on - strand, within Psest_4155 at 4337.496 kb on + strand, within Psest_4155
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 4,335,068 + +0.7 4,335,207 + Psest_4152 0.31 +0.8 4,335,207 + Psest_4152 0.31 -0.3 4,335,207 + Psest_4152 0.31 +2.9 4,335,207 + Psest_4152 0.31 +1.0 4,335,207 + Psest_4152 0.31 +3.7 4,335,207 + Psest_4152 0.31 -1.2 4,335,208 - Psest_4152 0.31 -0.3 4,335,213 + Psest_4152 0.32 -3.2 4,335,213 + Psest_4152 0.32 -0.7 4,335,214 - Psest_4152 0.33 +0.8 4,335,288 - Psest_4152 0.50 -1.8 4,335,346 - Psest_4152 0.63 -2.5 4,335,483 + -1.9 4,335,483 + +2.4 4,335,483 + +1.0 4,335,484 - -0.6 4,335,484 - -2.2 4,335,859 + -2.7 4,335,860 - +0.2 4,335,861 + -1.2 4,335,861 + +0.8 4,335,861 + +1.0 4,335,862 - +0.3 4,335,862 - -0.2 4,335,946 + +0.6 4,335,946 + +0.6 4,335,947 - -2.5 4,336,049 + Psest_4154 0.11 -2.1 4,336,049 + Psest_4154 0.11 -1.6 4,336,049 + Psest_4154 0.11 -0.4 4,336,049 + Psest_4154 0.11 -1.7 4,336,049 + Psest_4154 0.11 -0.3 4,336,050 - Psest_4154 0.11 +1.3 4,336,476 + Psest_4154 0.65 -2.5 4,336,476 + Psest_4154 0.65 -0.4 4,336,476 + Psest_4154 0.65 -2.1 4,336,476 + Psest_4154 0.65 -0.8 4,336,477 - Psest_4154 0.65 -1.0 4,336,690 + +1.3 4,336,690 + +1.3 4,336,690 + +1.8 4,336,690 + -0.8 4,336,691 - -2.3 4,336,746 - -1.9 4,336,752 + -0.4 4,336,752 + -1.9 4,336,752 + -1.0 4,336,752 + +1.2 4,336,753 - -1.9 4,336,841 - -1.5 4,336,898 - -1.0 4,336,908 + -0.6 4,336,908 + -0.2 4,336,908 + -1.6 4,336,908 + -0.9 4,336,909 - +1.4 4,336,909 - -1.8 4,336,951 + -3.1 4,336,952 - +4.6 4,336,952 - +1.8 4,336,952 - +2.0 4,336,952 - +0.8 4,336,952 - +2.2 4,336,952 - +0.2 4,337,238 + Psest_4155 0.37 +2.0 4,337,320 + Psest_4155 0.47 -2.7 4,337,339 + Psest_4155 0.49 -1.9 4,337,340 - Psest_4155 0.49 -0.2 4,337,340 - Psest_4155 0.49 +1.3 4,337,385 + Psest_4155 0.55 -0.4 4,337,385 + Psest_4155 0.55 +2.3 4,337,385 + Psest_4155 0.55 +0.1 4,337,385 + Psest_4155 0.55 +3.4 4,337,386 - Psest_4155 0.55 +1.0 4,337,386 - Psest_4155 0.55 +4.0 4,337,386 - Psest_4155 0.55 +2.2 4,337,413 - Psest_4155 0.58 -0.6 4,337,496 + Psest_4155 0.68 -1.9
Or see this region's nucleotide sequence