Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2020

Experiment: RCH2 grown on a colony of N2F3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2019 and Psest_2020 are separated by 87 nucleotidesPsest_2020 and Psest_2021 overlap by 4 nucleotides Psest_2019: Psest_2019 - adenylosuccinate lyase, at 2,138,064 to 2,139,434 _2019 Psest_2020: Psest_2020 - Uncharacterized protein involved in purine metabolism, at 2,139,522 to 2,140,160 _2020 Psest_2021: Psest_2021 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 2,140,157 to 2,141,284 _2021 Position (kb) 2139 2140 2141Strain fitness (log2 ratio) -3 -2 -1 0 1at 2139.483 kb on - strandat 2139.483 kb on - strandat 2139.483 kb on - strandat 2139.754 kb on + strand, within Psest_2020at 2139.754 kb on + strand, within Psest_2020at 2139.754 kb on + strand, within Psest_2020at 2139.756 kb on + strand, within Psest_2020at 2139.756 kb on + strand, within Psest_2020at 2139.757 kb on - strand, within Psest_2020at 2139.905 kb on - strand, within Psest_2020at 2139.910 kb on - strand, within Psest_2020at 2140.000 kb on + strand, within Psest_2020

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3
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2,139,483 - -2.8
2,139,483 - -0.5
2,139,483 - +1.3
2,139,754 + Psest_2020 0.36 -0.6
2,139,754 + Psest_2020 0.36 -1.6
2,139,754 + Psest_2020 0.36 -1.5
2,139,756 + Psest_2020 0.37 -0.1
2,139,756 + Psest_2020 0.37 +0.3
2,139,757 - Psest_2020 0.37 -0.4
2,139,905 - Psest_2020 0.60 -2.5
2,139,910 - Psest_2020 0.61 +0.1
2,140,000 + Psest_2020 0.75 -1.7

Or see this region's nucleotide sequence