Experiment: RCH2 grown on a colony of N2F3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0191 and Psest_0192 overlap by 4 nucleotides Psest_0192 and Psest_0193 are separated by 307 nucleotides Psest_0193 and Psest_0194 are separated by 178 nucleotides
Psest_0191: Psest_0191 - PAS domain S-box, at 186,472 to 188,259
_0191
Psest_0192: Psest_0192 - Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains, at 188,256 to 189,602
_0192
Psest_0193: Psest_0193 - conserved hypothetical protein, at 189,910 to 190,341
_0193
Psest_0194: Psest_0194 - hypothetical protein, at 190,520 to 190,756
_0194
Position (kb) 188
189
190 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 187.799 kb on + strand, within Psest_0191 at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.090 kb on - strand at 188.259 kb on + strand at 188.260 kb on - strand at 188.260 kb on - strand at 188.260 kb on - strand at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.686 kb on + strand, within Psest_0192 at 188.687 kb on - strand, within Psest_0192 at 188.800 kb on + strand, within Psest_0192 at 188.982 kb on + strand, within Psest_0192 at 189.286 kb on + strand, within Psest_0192 at 189.287 kb on - strand, within Psest_0192 at 189.287 kb on - strand, within Psest_0192 at 189.393 kb on + strand, within Psest_0192 at 189.393 kb on + strand, within Psest_0192 at 189.393 kb on + strand, within Psest_0192 at 189.393 kb on + strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.394 kb on - strand, within Psest_0192 at 189.503 kb on + strand at 189.503 kb on + strand at 189.503 kb on + strand at 189.503 kb on + strand at 189.505 kb on + strand at 189.505 kb on + strand at 189.536 kb on + strand at 189.537 kb on - strand at 189.650 kb on + strand at 189.788 kb on + strand at 189.789 kb on - strand at 189.974 kb on - strand, within Psest_0193 at 190.138 kb on + strand, within Psest_0193 at 190.474 kb on - strand at 190.505 kb on + strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of N2F3 remove 187,799 + Psest_0191 0.74 -1.1 188,089 + -0.1 188,089 + -2.9 188,089 + -1.2 188,089 + -0.3 188,089 + -2.3 188,089 + +1.8 188,090 - -0.8 188,259 + -1.7 188,260 - -1.9 188,260 - +1.6 188,260 - -4.0 188,530 + Psest_0192 0.20 +0.2 188,530 + Psest_0192 0.20 -0.8 188,530 + Psest_0192 0.20 -1.1 188,530 + Psest_0192 0.20 -0.2 188,531 - Psest_0192 0.20 -1.3 188,531 - Psest_0192 0.20 -0.8 188,531 - Psest_0192 0.20 -2.2 188,686 + Psest_0192 0.32 +0.2 188,687 - Psest_0192 0.32 +0.1 188,800 + Psest_0192 0.40 -1.0 188,982 + Psest_0192 0.54 -0.8 189,286 + Psest_0192 0.76 -3.0 189,287 - Psest_0192 0.77 -2.0 189,287 - Psest_0192 0.77 -2.1 189,393 + Psest_0192 0.84 -0.6 189,393 + Psest_0192 0.84 +1.8 189,393 + Psest_0192 0.84 -1.8 189,393 + Psest_0192 0.84 -3.2 189,394 - Psest_0192 0.84 -1.8 189,394 - Psest_0192 0.84 -1.0 189,394 - Psest_0192 0.84 -0.7 189,394 - Psest_0192 0.84 -0.6 189,394 - Psest_0192 0.84 -1.4 189,394 - Psest_0192 0.84 -2.0 189,394 - Psest_0192 0.84 -1.3 189,394 - Psest_0192 0.84 -0.7 189,503 + -2.1 189,503 + -2.3 189,503 + +0.7 189,503 + -3.2 189,505 + +0.5 189,505 + -0.3 189,536 + -2.8 189,537 - -1.8 189,650 + +0.8 189,788 + -0.0 189,789 - -2.3 189,974 - Psest_0193 0.15 -0.7 190,138 + Psest_0193 0.53 -1.3 190,474 - +0.2 190,505 + -0.3 190,506 - +1.2 190,506 - -1.6 190,506 - -1.8 190,506 - +0.4 190,506 - -0.1 190,506 - +1.9 190,506 - -0.4
Or see this region's nucleotide sequence