Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2795 and Psest_2796 are separated by 91 nucleotides Psest_2796 and Psest_2797 are separated by 23 nucleotides Psest_2797 and Psest_2798 are separated by 137 nucleotides Psest_2798 and Psest_2799 are separated by 223 nucleotides
Psest_2795: Psest_2795 - Uncharacterized conserved protein, at 2,987,278 to 2,987,976
_2795
Psest_2796: Psest_2796 - HNH endonuclease., at 2,988,068 to 2,988,412
_2796
Psest_2797: Psest_2797 - Universal stress protein UspA and related nucleotide-binding proteins, at 2,988,436 to 2,988,867
_2797
Psest_2798: Psest_2798 - Truncated hemoglobins, at 2,989,005 to 2,989,445
_2798
Psest_2799: Psest_2799 - Xanthosine triphosphate pyrophosphatase, at 2,989,669 to 2,990,217
_2799
Position (kb)
2988
2989 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 2987.832 kb on + strand, within Psest_2795 at 2987.957 kb on - strand at 2987.957 kb on - strand at 2987.957 kb on - strand at 2987.957 kb on - strand at 2987.957 kb on - strand at 2987.957 kb on - strand at 2987.989 kb on + strand at 2988.048 kb on + strand at 2988.048 kb on + strand at 2988.049 kb on - strand at 2988.049 kb on - strand at 2988.049 kb on - strand at 2988.074 kb on - strand at 2988.095 kb on + strand at 2988.140 kb on + strand, within Psest_2796 at 2988.140 kb on + strand, within Psest_2796 at 2988.140 kb on + strand, within Psest_2796 at 2988.141 kb on - strand, within Psest_2796 at 2988.141 kb on - strand, within Psest_2796 at 2988.141 kb on - strand, within Psest_2796 at 2988.272 kb on + strand, within Psest_2796 at 2988.273 kb on - strand, within Psest_2796 at 2988.314 kb on + strand, within Psest_2796 at 2988.704 kb on + strand, within Psest_2797 at 2988.704 kb on + strand, within Psest_2797 at 2988.722 kb on + strand, within Psest_2797 at 2988.722 kb on + strand, within Psest_2797 at 2988.723 kb on - strand, within Psest_2797 at 2988.723 kb on - strand, within Psest_2797 at 2988.723 kb on - strand, within Psest_2797 at 2988.731 kb on + strand, within Psest_2797 at 2988.809 kb on + strand, within Psest_2797 at 2988.809 kb on + strand, within Psest_2797 at 2988.809 kb on + strand, within Psest_2797 at 2988.809 kb on + strand, within Psest_2797 at 2988.810 kb on - strand, within Psest_2797 at 2988.810 kb on - strand, within Psest_2797 at 2988.810 kb on - strand, within Psest_2797 at 2988.810 kb on - strand, within Psest_2797 at 2988.810 kb on - strand, within Psest_2797 at 2988.845 kb on + strand at 2988.845 kb on + strand at 2988.845 kb on + strand at 2988.845 kb on + strand at 2988.845 kb on + strand at 2988.845 kb on + strand at 2988.845 kb on + strand at 2988.845 kb on + strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.846 kb on - strand at 2988.903 kb on - strand at 2988.903 kb on - strand at 2988.997 kb on + strand at 2988.997 kb on + strand at 2988.998 kb on - strand at 2989.101 kb on + strand, within Psest_2798 at 2989.102 kb on - strand, within Psest_2798 at 2989.102 kb on - strand, within Psest_2798 at 2989.236 kb on + strand, within Psest_2798 at 2989.342 kb on - strand, within Psest_2798 at 2989.342 kb on - strand, within Psest_2798 at 2989.508 kb on + strand at 2989.509 kb on - strand at 2989.605 kb on - strand at 2989.605 kb on - strand at 2989.605 kb on - strand at 2989.605 kb on - strand at 2989.662 kb on + strand at 2989.662 kb on + strand at 2989.662 kb on + strand at 2989.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 2,987,832 + Psest_2795 0.79 -1.2 2,987,957 - +2.7 2,987,957 - -0.2 2,987,957 - +2.2 2,987,957 - -0.3 2,987,957 - -2.3 2,987,957 - +3.1 2,987,989 + +0.5 2,988,048 + +1.7 2,988,048 + -2.1 2,988,049 - +3.1 2,988,049 - +1.4 2,988,049 - +4.7 2,988,074 - -0.9 2,988,095 + +0.4 2,988,140 + Psest_2796 0.21 +1.6 2,988,140 + Psest_2796 0.21 -1.5 2,988,140 + Psest_2796 0.21 -2.2 2,988,141 - Psest_2796 0.21 -1.2 2,988,141 - Psest_2796 0.21 -2.7 2,988,141 - Psest_2796 0.21 +0.9 2,988,272 + Psest_2796 0.59 -1.1 2,988,273 - Psest_2796 0.59 +0.3 2,988,314 + Psest_2796 0.71 +1.4 2,988,704 + Psest_2797 0.62 +0.2 2,988,704 + Psest_2797 0.62 +0.8 2,988,722 + Psest_2797 0.66 +1.1 2,988,722 + Psest_2797 0.66 +3.1 2,988,723 - Psest_2797 0.66 +2.5 2,988,723 - Psest_2797 0.66 -0.4 2,988,723 - Psest_2797 0.66 +1.7 2,988,731 + Psest_2797 0.68 +0.2 2,988,809 + Psest_2797 0.86 +3.6 2,988,809 + Psest_2797 0.86 +1.7 2,988,809 + Psest_2797 0.86 -0.1 2,988,809 + Psest_2797 0.86 +2.2 2,988,810 - Psest_2797 0.87 -1.7 2,988,810 - Psest_2797 0.87 +0.5 2,988,810 - Psest_2797 0.87 +0.8 2,988,810 - Psest_2797 0.87 +4.8 2,988,810 - Psest_2797 0.87 +2.4 2,988,845 + +7.9 2,988,845 + +2.1 2,988,845 + -1.3 2,988,845 + -0.0 2,988,845 + -1.8 2,988,845 + -1.9 2,988,845 + -2.3 2,988,845 + +4.5 2,988,846 - -2.1 2,988,846 - -1.3 2,988,846 - -0.9 2,988,846 - +0.3 2,988,846 - +0.3 2,988,846 - +1.2 2,988,846 - -1.6 2,988,846 - +1.5 2,988,846 - +1.4 2,988,846 - -1.9 2,988,846 - -0.7 2,988,846 - +0.4 2,988,846 - +1.7 2,988,903 - -2.5 2,988,903 - +4.8 2,988,997 + -0.5 2,988,997 + -0.9 2,988,998 - -2.1 2,989,101 + Psest_2798 0.22 -2.1 2,989,102 - Psest_2798 0.22 +1.1 2,989,102 - Psest_2798 0.22 -2.0 2,989,236 + Psest_2798 0.52 +0.1 2,989,342 - Psest_2798 0.76 +2.5 2,989,342 - Psest_2798 0.76 -2.1 2,989,508 + +0.9 2,989,509 - -0.1 2,989,605 - -3.7 2,989,605 - -0.8 2,989,605 - -0.0 2,989,605 - -1.8 2,989,662 + -2.6 2,989,662 + -2.4 2,989,662 + -1.1 2,989,668 + -0.7
Or see this region's nucleotide sequence