Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3587 and Pf6N2E2_3588 are separated by 143 nucleotides Pf6N2E2_3588 and Pf6N2E2_3589 are separated by 61 nucleotides Pf6N2E2_3589 and Pf6N2E2_3590 are separated by 68 nucleotides Pf6N2E2_3590 and Pf6N2E2_3591 are separated by 72 nucleotides Pf6N2E2_3591 and Pf6N2E2_3592 are separated by 142 nucleotides
Pf6N2E2_3587 - Azurin, at 4,179,016 to 4,179,462
_3587
Pf6N2E2_3588 - hypothetical protein, at 4,179,606 to 4,179,743
_3588
Pf6N2E2_3589 - Lysine decarboxylase family, at 4,179,805 to 4,180,392
_3589
Pf6N2E2_3590 - FIG00956548: hypothetical protein, at 4,180,461 to 4,180,649
_3590
Pf6N2E2_3591 - NUDIX hydrolase, at 4,180,722 to 4,181,099
_3591
Pf6N2E2_3592 - Protein containing transglutaminase-like domain, putative cysteine protease, at 4,181,242 to 4,182,135
_3592
Position (kb)
4179
4180
4181 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4178.981 kb on - strand at 4179.080 kb on + strand, within Pf6N2E2_3587 at 4179.081 kb on - strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.188 kb on + strand, within Pf6N2E2_3587 at 4179.189 kb on - strand, within Pf6N2E2_3587 at 4179.189 kb on - strand, within Pf6N2E2_3587 at 4179.440 kb on - strand at 4179.534 kb on + strand at 4179.564 kb on + strand at 4179.564 kb on + strand at 4179.571 kb on - strand at 4179.571 kb on - strand at 4179.618 kb on + strand at 4179.619 kb on - strand at 4179.619 kb on - strand at 4179.619 kb on - strand at 4179.619 kb on - strand at 4179.673 kb on + strand, within Pf6N2E2_3588 at 4179.673 kb on + strand, within Pf6N2E2_3588 at 4179.674 kb on - strand, within Pf6N2E2_3588 at 4179.718 kb on - strand, within Pf6N2E2_3588 at 4179.741 kb on + strand at 4179.771 kb on + strand at 4179.799 kb on + strand at 4179.799 kb on + strand at 4179.799 kb on + strand at 4179.799 kb on + strand at 4179.800 kb on - strand at 4179.800 kb on - strand at 4179.800 kb on - strand at 4179.800 kb on - strand at 4179.827 kb on + strand at 4179.828 kb on - strand at 4179.828 kb on - strand at 4179.862 kb on + strand at 4179.863 kb on - strand at 4180.226 kb on - strand, within Pf6N2E2_3589 at 4180.255 kb on - strand, within Pf6N2E2_3589 at 4180.419 kb on + strand at 4180.477 kb on - strand at 4180.477 kb on - strand at 4180.671 kb on + strand at 4180.671 kb on + strand at 4180.671 kb on + strand at 4180.671 kb on + strand at 4180.672 kb on - strand at 4180.672 kb on - strand at 4180.672 kb on - strand at 4180.672 kb on - strand at 4180.672 kb on - strand at 4180.709 kb on + strand at 4180.709 kb on + strand at 4180.861 kb on + strand, within Pf6N2E2_3591 at 4180.862 kb on - strand, within Pf6N2E2_3591 at 4180.862 kb on - strand, within Pf6N2E2_3591 at 4180.862 kb on - strand, within Pf6N2E2_3591 at 4180.862 kb on - strand, within Pf6N2E2_3591 at 4180.906 kb on + strand, within Pf6N2E2_3591 at 4180.906 kb on + strand, within Pf6N2E2_3591 at 4180.955 kb on + strand, within Pf6N2E2_3591 at 4181.124 kb on + strand at 4181.124 kb on + strand at 4181.124 kb on + strand at 4181.124 kb on + strand at 4181.124 kb on + strand at 4181.125 kb on - strand at 4181.125 kb on - strand at 4181.125 kb on - strand at 4181.125 kb on - strand at 4181.134 kb on + strand at 4181.222 kb on + strand at 4181.222 kb on + strand at 4181.222 kb on + strand at 4181.222 kb on + strand at 4181.223 kb on - strand at 4181.223 kb on - strand at 4181.223 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 4,178,981 - -0.3 4,179,080 + Pf6N2E2_3587 0.14 +0.5 4,179,081 - Pf6N2E2_3587 0.15 +1.9 4,179,188 + Pf6N2E2_3587 0.38 +0.9 4,179,188 + Pf6N2E2_3587 0.38 +0.4 4,179,188 + Pf6N2E2_3587 0.38 -0.1 4,179,188 + Pf6N2E2_3587 0.38 +0.5 4,179,189 - Pf6N2E2_3587 0.39 -0.0 4,179,189 - Pf6N2E2_3587 0.39 +0.4 4,179,440 - +0.5 4,179,534 + +0.8 4,179,564 + -1.6 4,179,564 + +0.0 4,179,571 - -0.1 4,179,571 - -0.6 4,179,618 + +0.9 4,179,619 - +0.1 4,179,619 - +0.4 4,179,619 - -0.5 4,179,619 - +0.4 4,179,673 + Pf6N2E2_3588 0.49 -0.9 4,179,673 + Pf6N2E2_3588 0.49 +0.5 4,179,674 - Pf6N2E2_3588 0.49 +0.5 4,179,718 - Pf6N2E2_3588 0.81 +1.2 4,179,741 + -0.6 4,179,771 + +2.4 4,179,799 + -0.6 4,179,799 + +1.6 4,179,799 + -0.2 4,179,799 + +0.4 4,179,800 - +0.0 4,179,800 - +0.6 4,179,800 - +0.7 4,179,800 - +0.3 4,179,827 + +0.5 4,179,828 - +0.7 4,179,828 - -0.6 4,179,862 + +0.5 4,179,863 - -0.6 4,180,226 - Pf6N2E2_3589 0.72 -1.1 4,180,255 - Pf6N2E2_3589 0.77 -0.5 4,180,419 + +0.0 4,180,477 - -0.5 4,180,477 - -0.2 4,180,671 + +0.6 4,180,671 + +0.8 4,180,671 + -0.6 4,180,671 + -0.2 4,180,672 - +0.6 4,180,672 - +1.9 4,180,672 - -2.5 4,180,672 - +2.4 4,180,672 - +0.4 4,180,709 + -1.6 4,180,709 + -1.0 4,180,861 + Pf6N2E2_3591 0.37 +0.4 4,180,862 - Pf6N2E2_3591 0.37 +0.4 4,180,862 - Pf6N2E2_3591 0.37 -0.2 4,180,862 - Pf6N2E2_3591 0.37 +0.6 4,180,862 - Pf6N2E2_3591 0.37 -0.1 4,180,906 + Pf6N2E2_3591 0.49 +1.4 4,180,906 + Pf6N2E2_3591 0.49 -0.2 4,180,955 + Pf6N2E2_3591 0.62 +1.2 4,181,124 + +0.2 4,181,124 + +0.9 4,181,124 + +0.4 4,181,124 + +0.4 4,181,124 + +0.4 4,181,125 - +0.1 4,181,125 - +0.1 4,181,125 - -0.6 4,181,125 - +0.1 4,181,134 + -0.6 4,181,222 + -0.8 4,181,222 + +0.1 4,181,222 + -0.6 4,181,222 + -0.2 4,181,223 - +0.4 4,181,223 - +0.2 4,181,223 - -0.4
Or see this region's nucleotide sequence