Experiment: LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21830 and BWI76_RS21835 are separated by 3 nucleotides BWI76_RS21835 and BWI76_RS21840 are separated by 464 nucleotides
BWI76_RS21830: BWI76_RS21830 - hypothetical protein, at 4,559,650 to 4,561,785
_RS21830
BWI76_RS21835: BWI76_RS21835 - hypothetical protein, at 4,561,789 to 4,562,940
_RS21835
BWI76_RS21840: BWI76_RS21840 - IS110 family transposase, at 4,563,405 to 4,564,400
_RS21840
Position (kb) 4561
4562
4563 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4560.800 kb on - strand, within BWI76_RS21830 at 4560.813 kb on - strand, within BWI76_RS21830 at 4560.815 kb on + strand, within BWI76_RS21830 at 4560.829 kb on + strand, within BWI76_RS21830 at 4560.834 kb on + strand, within BWI76_RS21830 at 4560.842 kb on - strand, within BWI76_RS21830 at 4560.848 kb on + strand, within BWI76_RS21830 at 4560.866 kb on - strand, within BWI76_RS21830 at 4560.868 kb on + strand, within BWI76_RS21830 at 4560.868 kb on + strand, within BWI76_RS21830 at 4560.868 kb on + strand, within BWI76_RS21830 at 4560.877 kb on - strand, within BWI76_RS21830 at 4560.887 kb on + strand, within BWI76_RS21830 at 4560.895 kb on - strand, within BWI76_RS21830 at 4560.895 kb on - strand, within BWI76_RS21830 at 4560.948 kb on - strand, within BWI76_RS21830 at 4560.956 kb on + strand, within BWI76_RS21830 at 4560.998 kb on + strand, within BWI76_RS21830 at 4561.056 kb on + strand, within BWI76_RS21830 at 4561.064 kb on - strand, within BWI76_RS21830 at 4561.083 kb on + strand, within BWI76_RS21830 at 4561.113 kb on + strand, within BWI76_RS21830 at 4561.126 kb on - strand, within BWI76_RS21830 at 4561.126 kb on - strand, within BWI76_RS21830 at 4561.126 kb on - strand, within BWI76_RS21830 at 4561.126 kb on - strand, within BWI76_RS21830 at 4561.138 kb on + strand, within BWI76_RS21830 at 4561.138 kb on + strand, within BWI76_RS21830 at 4561.146 kb on - strand, within BWI76_RS21830 at 4561.146 kb on - strand, within BWI76_RS21830 at 4561.146 kb on - strand, within BWI76_RS21830 at 4561.157 kb on + strand, within BWI76_RS21830 at 4561.186 kb on + strand, within BWI76_RS21830 at 4561.260 kb on - strand, within BWI76_RS21830 at 4561.370 kb on + strand, within BWI76_RS21830 at 4561.378 kb on - strand, within BWI76_RS21830 at 4561.515 kb on - strand, within BWI76_RS21830 at 4561.573 kb on + strand at 4561.591 kb on - strand at 4561.608 kb on - strand at 4561.608 kb on - strand at 4561.704 kb on + strand at 4561.712 kb on - strand at 4561.722 kb on - strand at 4561.900 kb on - strand at 4561.979 kb on - strand, within BWI76_RS21835 at 4562.148 kb on - strand, within BWI76_RS21835 at 4562.353 kb on - strand, within BWI76_RS21835 at 4562.358 kb on - strand, within BWI76_RS21835 at 4562.577 kb on + strand, within BWI76_RS21835 at 4562.577 kb on + strand, within BWI76_RS21835 at 4562.582 kb on + strand, within BWI76_RS21835 at 4562.585 kb on - strand, within BWI76_RS21835 at 4562.590 kb on - strand, within BWI76_RS21835 at 4562.590 kb on - strand, within BWI76_RS21835 at 4562.717 kb on + strand, within BWI76_RS21835 at 4562.725 kb on - strand, within BWI76_RS21835 at 4562.725 kb on - strand, within BWI76_RS21835 at 4562.735 kb on + strand, within BWI76_RS21835 at 4562.860 kb on - strand at 4563.094 kb on - strand at 4563.112 kb on + strand at 4563.127 kb on - strand at 4563.397 kb on + strand at 4563.423 kb on + strand at 4563.439 kb on + strand at 4563.474 kb on - strand at 4563.535 kb on + strand, within BWI76_RS21840 at 4563.535 kb on + strand, within BWI76_RS21840 at 4563.535 kb on + strand, within BWI76_RS21840 at 4563.541 kb on + strand, within BWI76_RS21840 at 4563.541 kb on + strand, within BWI76_RS21840 at 4563.594 kb on - strand, within BWI76_RS21840 at 4563.606 kb on + strand, within BWI76_RS21840 at 4563.712 kb on + strand, within BWI76_RS21840 at 4563.721 kb on + strand, within BWI76_RS21840 at 4563.721 kb on - strand, within BWI76_RS21840 at 4563.883 kb on + strand, within BWI76_RS21840
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,560,800 - BWI76_RS21830 0.54 -0.3 4,560,813 - BWI76_RS21830 0.54 -0.4 4,560,815 + BWI76_RS21830 0.55 -0.8 4,560,829 + BWI76_RS21830 0.55 +1.3 4,560,834 + BWI76_RS21830 0.55 -0.7 4,560,842 - BWI76_RS21830 0.56 -0.2 4,560,848 + BWI76_RS21830 0.56 -2.6 4,560,866 - BWI76_RS21830 0.57 +0.0 4,560,868 + BWI76_RS21830 0.57 +0.7 4,560,868 + BWI76_RS21830 0.57 -1.3 4,560,868 + BWI76_RS21830 0.57 +1.0 4,560,877 - BWI76_RS21830 0.57 +0.3 4,560,887 + BWI76_RS21830 0.58 +0.4 4,560,895 - BWI76_RS21830 0.58 +0.9 4,560,895 - BWI76_RS21830 0.58 +1.0 4,560,948 - BWI76_RS21830 0.61 -0.1 4,560,956 + BWI76_RS21830 0.61 -1.0 4,560,998 + BWI76_RS21830 0.63 +1.1 4,561,056 + BWI76_RS21830 0.66 -1.3 4,561,064 - BWI76_RS21830 0.66 +0.5 4,561,083 + BWI76_RS21830 0.67 +0.4 4,561,113 + BWI76_RS21830 0.68 +0.7 4,561,126 - BWI76_RS21830 0.69 +0.7 4,561,126 - BWI76_RS21830 0.69 -0.2 4,561,126 - BWI76_RS21830 0.69 +1.1 4,561,126 - BWI76_RS21830 0.69 -1.6 4,561,138 + BWI76_RS21830 0.70 -3.0 4,561,138 + BWI76_RS21830 0.70 -0.8 4,561,146 - BWI76_RS21830 0.70 +0.4 4,561,146 - BWI76_RS21830 0.70 -0.8 4,561,146 - BWI76_RS21830 0.70 -1.3 4,561,157 + BWI76_RS21830 0.71 -1.6 4,561,186 + BWI76_RS21830 0.72 +0.7 4,561,260 - BWI76_RS21830 0.75 -0.1 4,561,370 + BWI76_RS21830 0.81 -0.1 4,561,378 - BWI76_RS21830 0.81 +0.2 4,561,515 - BWI76_RS21830 0.87 +2.6 4,561,573 + +1.5 4,561,591 - +0.4 4,561,608 - +0.4 4,561,608 - -0.3 4,561,704 + +0.1 4,561,712 - +0.2 4,561,722 - +0.4 4,561,900 - +0.9 4,561,979 - BWI76_RS21835 0.16 -0.2 4,562,148 - BWI76_RS21835 0.31 +0.3 4,562,353 - BWI76_RS21835 0.49 -1.2 4,562,358 - BWI76_RS21835 0.49 -1.1 4,562,577 + BWI76_RS21835 0.68 -0.0 4,562,577 + BWI76_RS21835 0.68 -0.2 4,562,582 + BWI76_RS21835 0.69 -0.5 4,562,585 - BWI76_RS21835 0.69 -0.4 4,562,590 - BWI76_RS21835 0.70 +0.1 4,562,590 - BWI76_RS21835 0.70 -0.3 4,562,717 + BWI76_RS21835 0.81 +0.1 4,562,725 - BWI76_RS21835 0.81 +0.2 4,562,725 - BWI76_RS21835 0.81 -0.5 4,562,735 + BWI76_RS21835 0.82 -0.2 4,562,860 - +0.2 4,563,094 - +0.3 4,563,112 + +0.4 4,563,127 - -0.7 4,563,397 + -0.3 4,563,423 + +0.6 4,563,439 + -0.5 4,563,474 - +0.2 4,563,535 + BWI76_RS21840 0.13 +0.9 4,563,535 + BWI76_RS21840 0.13 -0.5 4,563,535 + BWI76_RS21840 0.13 -0.1 4,563,541 + BWI76_RS21840 0.14 +0.3 4,563,541 + BWI76_RS21840 0.14 -0.1 4,563,594 - BWI76_RS21840 0.19 +0.3 4,563,606 + BWI76_RS21840 0.20 -1.8 4,563,712 + BWI76_RS21840 0.31 +3.3 4,563,721 + BWI76_RS21840 0.32 +0.2 4,563,721 - BWI76_RS21840 0.32 -0.8 4,563,883 + BWI76_RS21840 0.48 +1.0
Or see this region's nucleotide sequence