Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25165

Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25155 and BWI76_RS25160 are separated by 152 nucleotidesBWI76_RS25160 and BWI76_RS25165 are separated by 233 nucleotidesBWI76_RS25165 and BWI76_RS25170 are separated by 46 nucleotides BWI76_RS25155: BWI76_RS25155 - RNA-binding protein, at 5,235,174 to 5,235,467 _RS25155 BWI76_RS25160: BWI76_RS25160 - transcription elongation factor GreA, at 5,235,620 to 5,236,096 _RS25160 BWI76_RS25165: BWI76_RS25165 - serine-type D-Ala-D-Ala carboxypeptidase, at 5,236,330 to 5,237,763 _RS25165 BWI76_RS25170: BWI76_RS25170 - GTPase ObgE, at 5,237,810 to 5,238,982 _RS25170 Position (kb) 5236 5237 5238Strain fitness (log2 ratio) -2 -1 0 1at 5235.956 kb on - strand, within BWI76_RS25160at 5236.079 kb on + strandat 5236.129 kb on + strandat 5236.208 kb on - strandat 5236.375 kb on - strandat 5236.414 kb on - strandat 5236.548 kb on + strand, within BWI76_RS25165at 5236.617 kb on + strand, within BWI76_RS25165at 5236.727 kb on + strand, within BWI76_RS25165at 5236.752 kb on - strand, within BWI76_RS25165at 5236.868 kb on - strand, within BWI76_RS25165at 5237.142 kb on - strand, within BWI76_RS25165at 5237.292 kb on - strand, within BWI76_RS25165at 5237.347 kb on - strand, within BWI76_RS25165at 5237.524 kb on + strand, within BWI76_RS25165at 5237.547 kb on - strand, within BWI76_RS25165at 5237.607 kb on - strand, within BWI76_RS25165at 5237.913 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
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5,235,956 - BWI76_RS25160 0.70 +0.5
5,236,079 + +0.7
5,236,129 + -1.2
5,236,208 - -0.3
5,236,375 - +0.4
5,236,414 - +0.9
5,236,548 + BWI76_RS25165 0.15 -1.2
5,236,617 + BWI76_RS25165 0.20 +0.4
5,236,727 + BWI76_RS25165 0.28 +1.1
5,236,752 - BWI76_RS25165 0.29 +1.4
5,236,868 - BWI76_RS25165 0.38 +0.1
5,237,142 - BWI76_RS25165 0.57 -1.9
5,237,292 - BWI76_RS25165 0.67 +0.8
5,237,347 - BWI76_RS25165 0.71 +0.8
5,237,524 + BWI76_RS25165 0.83 +0.6
5,237,547 - BWI76_RS25165 0.85 +0.9
5,237,607 - BWI76_RS25165 0.89 +0.6
5,237,913 - -0.8

Or see this region's nucleotide sequence