Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16450 and BWI76_RS16455 overlap by 8 nucleotides BWI76_RS16455 and BWI76_RS16460 are separated by 59 nucleotides BWI76_RS16460 and BWI76_RS16465 are separated by 48 nucleotides BWI76_RS16465 and BWI76_RS16470 are separated by 142 nucleotides
BWI76_RS16450: BWI76_RS16450 - fusaric acid resistance protein, at 3,411,518 to 3,413,542
_RS16450
BWI76_RS16455: BWI76_RS16455 - superoxide dismutase, at 3,413,535 to 3,414,056
_RS16455
BWI76_RS16460: BWI76_RS16460 - oxidoreductase, at 3,414,116 to 3,415,012
_RS16460
BWI76_RS16465: BWI76_RS16465 - DUF1289 domain-containing protein, at 3,415,061 to 3,415,300
_RS16465
BWI76_RS16470: BWI76_RS16470 - transcriptional repressor NemR, at 3,415,443 to 3,416,042
_RS16470
Position (kb)
3414
3415
3416 Strain fitness (log2 ratio)
-1
0
1 at 3413.343 kb on - strand at 3413.361 kb on + strand at 3413.382 kb on + strand at 3413.390 kb on - strand at 3413.729 kb on + strand, within BWI76_RS16455 at 3413.749 kb on + strand, within BWI76_RS16455 at 3414.262 kb on + strand, within BWI76_RS16460 at 3414.272 kb on - strand, within BWI76_RS16460 at 3414.814 kb on - strand, within BWI76_RS16460 at 3414.983 kb on + strand at 3415.448 kb on + strand at 3415.499 kb on + strand at 3415.619 kb on + strand, within BWI76_RS16470
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,413,343 - +0.2 3,413,361 + -0.6 3,413,382 + +0.3 3,413,390 - +0.1 3,413,729 + BWI76_RS16455 0.37 +0.3 3,413,749 + BWI76_RS16455 0.41 -0.6 3,414,262 + BWI76_RS16460 0.16 +0.2 3,414,272 - BWI76_RS16460 0.17 +0.6 3,414,814 - BWI76_RS16460 0.78 +0.8 3,414,983 + -0.7 3,415,448 + -1.3 3,415,499 + +0.8 3,415,619 + BWI76_RS16470 0.29 -0.4
Or see this region's nucleotide sequence