Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01885 and BWI76_RS01890 are separated by 90 nucleotides BWI76_RS01890 and BWI76_RS01895 are separated by 192 nucleotides BWI76_RS01895 and BWI76_RS01900 are separated by 107 nucleotides
BWI76_RS01885: BWI76_RS01885 - CsbD family protein, at 385,233 to 385,445
_RS01885
BWI76_RS01890: BWI76_RS01890 - transcriptional regulator Zur, at 385,536 to 386,051
_RS01890
BWI76_RS01895: BWI76_RS01895 - cupin, at 386,244 to 386,732
_RS01895
BWI76_RS01900: BWI76_RS01900 - tRNA-dihydrouridine synthase A, at 386,840 to 387,835
_RS01900
Position (kb) 386
387 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 385.328 kb on + strand, within BWI76_RS01885 at 385.599 kb on - strand, within BWI76_RS01890 at 385.746 kb on + strand, within BWI76_RS01890 at 385.913 kb on + strand, within BWI76_RS01890 at 385.921 kb on - strand, within BWI76_RS01890 at 385.921 kb on - strand, within BWI76_RS01890 at 385.937 kb on + strand, within BWI76_RS01890 at 386.040 kb on + strand at 386.186 kb on - strand at 386.208 kb on + strand at 386.209 kb on - strand at 386.213 kb on + strand at 386.295 kb on - strand, within BWI76_RS01895 at 386.314 kb on + strand, within BWI76_RS01895 at 386.322 kb on - strand, within BWI76_RS01895 at 386.475 kb on - strand, within BWI76_RS01895 at 387.444 kb on - strand, within BWI76_RS01900 at 387.475 kb on - strand, within BWI76_RS01900 at 387.505 kb on + strand, within BWI76_RS01900 at 387.657 kb on + strand, within BWI76_RS01900 at 387.675 kb on + strand, within BWI76_RS01900
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 385,328 + BWI76_RS01885 0.45 -0.4 385,599 - BWI76_RS01890 0.12 -0.0 385,746 + BWI76_RS01890 0.41 -0.3 385,913 + BWI76_RS01890 0.73 +1.0 385,921 - BWI76_RS01890 0.75 +0.0 385,921 - BWI76_RS01890 0.75 -1.0 385,937 + BWI76_RS01890 0.78 +1.2 386,040 + +1.0 386,186 - -2.9 386,208 + +0.6 386,209 - +0.8 386,213 + +0.7 386,295 - BWI76_RS01895 0.10 +0.7 386,314 + BWI76_RS01895 0.14 +0.7 386,322 - BWI76_RS01895 0.16 +0.5 386,475 - BWI76_RS01895 0.47 +1.3 387,444 - BWI76_RS01900 0.61 -0.6 387,475 - BWI76_RS01900 0.64 -0.7 387,505 + BWI76_RS01900 0.67 -0.8 387,657 + BWI76_RS01900 0.82 -0.2 387,675 + BWI76_RS01900 0.84 +0.8
Or see this region's nucleotide sequence