Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00325 and BWI76_RS00330 overlap by 4 nucleotides BWI76_RS00330 and BWI76_RS00335 are separated by 71 nucleotides BWI76_RS00335 and BWI76_RS00340 are separated by 75 nucleotides BWI76_RS00340 and BWI76_RS00345 are separated by 27 nucleotides BWI76_RS00345 and BWI76_RS00350 are separated by 49 nucleotides
BWI76_RS00325: BWI76_RS00325 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 69,712 to 70,218
_RS00325
BWI76_RS00330: BWI76_RS00330 - molybdenum cofactor guanylyltransferase, at 70,215 to 70,796
_RS00330
BWI76_RS00335: BWI76_RS00335 - hypothetical protein, at 70,868 to 71,137
_RS00335
BWI76_RS00340: BWI76_RS00340 - stress response kinase A, at 71,213 to 72,199
_RS00340
BWI76_RS00345: BWI76_RS00345 - protein disulfide oxidoreductase DsbA, at 72,227 to 72,850
_RS00345
BWI76_RS00350: BWI76_RS00350 - acyltransferase, at 72,900 to 73,799
_RS00350
Position (kb) 71
72
73 Strain fitness (log2 ratio) -2
-1
0
1 at 70.364 kb on - strand, within BWI76_RS00330 at 70.386 kb on + strand, within BWI76_RS00330 at 70.525 kb on + strand, within BWI76_RS00330 at 70.791 kb on - strand at 70.829 kb on - strand at 70.835 kb on + strand at 70.844 kb on - strand at 70.913 kb on + strand, within BWI76_RS00335 at 71.051 kb on - strand, within BWI76_RS00335 at 71.115 kb on - strand at 71.188 kb on + strand at 71.224 kb on + strand at 71.232 kb on - strand at 71.303 kb on - strand at 71.317 kb on - strand, within BWI76_RS00340 at 71.529 kb on + strand, within BWI76_RS00340 at 71.637 kb on - strand, within BWI76_RS00340 at 72.120 kb on + strand at 72.151 kb on + strand at 72.151 kb on + strand at 72.151 kb on + strand at 72.151 kb on + strand at 72.151 kb on + strand at 72.158 kb on + strand at 72.159 kb on - strand at 72.159 kb on - strand at 72.159 kb on - strand at 72.159 kb on - strand at 72.159 kb on - strand at 72.201 kb on - strand at 72.307 kb on + strand, within BWI76_RS00345 at 72.339 kb on + strand, within BWI76_RS00345 at 72.510 kb on - strand, within BWI76_RS00345 at 72.510 kb on - strand, within BWI76_RS00345 at 72.592 kb on - strand, within BWI76_RS00345 at 72.592 kb on - strand, within BWI76_RS00345 at 72.661 kb on - strand, within BWI76_RS00345 at 72.745 kb on - strand, within BWI76_RS00345 at 72.822 kb on + strand at 72.830 kb on - strand at 72.832 kb on - strand at 72.875 kb on + strand at 72.875 kb on + strand at 72.875 kb on + strand at 72.883 kb on - strand at 72.883 kb on - strand at 72.883 kb on - strand at 72.959 kb on + strand at 72.959 kb on + strand at 72.967 kb on - strand at 73.004 kb on + strand, within BWI76_RS00350 at 73.019 kb on + strand, within BWI76_RS00350 at 73.034 kb on - strand at 73.047 kb on + strand, within BWI76_RS00350 at 73.047 kb on - strand, within BWI76_RS00350 at 73.111 kb on + strand, within BWI76_RS00350 at 73.111 kb on + strand, within BWI76_RS00350 at 73.111 kb on + strand, within BWI76_RS00350 at 73.111 kb on + strand, within BWI76_RS00350 at 73.121 kb on + strand, within BWI76_RS00350 at 73.129 kb on - strand, within BWI76_RS00350 at 73.129 kb on - strand, within BWI76_RS00350 at 73.129 kb on - strand, within BWI76_RS00350 at 73.136 kb on + strand, within BWI76_RS00350 at 73.152 kb on + strand, within BWI76_RS00350 at 73.169 kb on - strand, within BWI76_RS00350
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 70,364 - BWI76_RS00330 0.26 +0.3 70,386 + BWI76_RS00330 0.29 +0.3 70,525 + BWI76_RS00330 0.53 +0.4 70,791 - +0.1 70,829 - -0.3 70,835 + -0.2 70,844 - -0.8 70,913 + BWI76_RS00335 0.17 +0.5 71,051 - BWI76_RS00335 0.68 +0.5 71,115 - +1.0 71,188 + -0.3 71,224 + -2.0 71,232 - +0.5 71,303 - -0.6 71,317 - BWI76_RS00340 0.11 +1.0 71,529 + BWI76_RS00340 0.32 +0.3 71,637 - BWI76_RS00340 0.43 +0.6 72,120 + -0.6 72,151 + +0.0 72,151 + -2.3 72,151 + +0.3 72,151 + +0.2 72,151 + -1.6 72,158 + -0.3 72,159 - -1.3 72,159 - -0.1 72,159 - +0.4 72,159 - -0.1 72,159 - -0.6 72,201 - +0.9 72,307 + BWI76_RS00345 0.13 +0.6 72,339 + BWI76_RS00345 0.18 -1.0 72,510 - BWI76_RS00345 0.45 +0.0 72,510 - BWI76_RS00345 0.45 -1.6 72,592 - BWI76_RS00345 0.58 -1.0 72,592 - BWI76_RS00345 0.58 -1.9 72,661 - BWI76_RS00345 0.70 -0.8 72,745 - BWI76_RS00345 0.83 +0.6 72,822 + +0.0 72,830 - +1.0 72,832 - -0.1 72,875 + +1.4 72,875 + -1.6 72,875 + -0.4 72,883 - +0.1 72,883 - -2.0 72,883 - +0.0 72,959 + +0.8 72,959 + -1.3 72,967 - +0.2 73,004 + BWI76_RS00350 0.12 +0.1 73,019 + BWI76_RS00350 0.13 +0.3 73,034 - +1.0 73,047 + BWI76_RS00350 0.16 +0.7 73,047 - BWI76_RS00350 0.16 +0.9 73,111 + BWI76_RS00350 0.23 +0.3 73,111 + BWI76_RS00350 0.23 -1.3 73,111 + BWI76_RS00350 0.23 -0.4 73,111 + BWI76_RS00350 0.23 +0.0 73,121 + BWI76_RS00350 0.25 -0.6 73,129 - BWI76_RS00350 0.25 -1.2 73,129 - BWI76_RS00350 0.25 -0.4 73,129 - BWI76_RS00350 0.25 +0.3 73,136 + BWI76_RS00350 0.26 +0.0 73,152 + BWI76_RS00350 0.28 +0.0 73,169 - BWI76_RS00350 0.30 -0.2
Or see this region's nucleotide sequence