Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20030

Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20025 and BWI76_RS20030 overlap by 14 nucleotidesBWI76_RS20030 and BWI76_RS20035 are separated by 134 nucleotides BWI76_RS20025: BWI76_RS20025 - HAMP domain-containing histidine kinase, at 4,195,340 to 4,196,755 _RS20025 BWI76_RS20030: BWI76_RS20030 - DNA-binding response regulator, at 4,196,742 to 4,197,416 _RS20030 BWI76_RS20035: BWI76_RS20035 - hypothetical protein, at 4,197,551 to 4,198,531 _RS20035 Position (kb) 4196 4197 4198Strain fitness (log2 ratio) -1 0 1 2 3at 4195.956 kb on + strand, within BWI76_RS20025at 4196.001 kb on + strand, within BWI76_RS20025at 4196.305 kb on - strand, within BWI76_RS20025at 4196.528 kb on + strand, within BWI76_RS20025at 4196.762 kb on + strandat 4196.811 kb on + strand, within BWI76_RS20030at 4196.846 kb on + strand, within BWI76_RS20030at 4197.056 kb on - strand, within BWI76_RS20030at 4197.156 kb on - strand, within BWI76_RS20030at 4197.233 kb on + strand, within BWI76_RS20030at 4197.527 kb on + strandat 4197.603 kb on - strandat 4197.610 kb on + strandat 4197.613 kb on - strandat 4197.705 kb on + strand, within BWI76_RS20035at 4197.852 kb on + strand, within BWI76_RS20035at 4197.852 kb on + strand, within BWI76_RS20035at 4198.263 kb on + strand, within BWI76_RS20035at 4198.393 kb on + strand, within BWI76_RS20035

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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4,195,956 + BWI76_RS20025 0.44 +0.6
4,196,001 + BWI76_RS20025 0.47 +1.2
4,196,305 - BWI76_RS20025 0.68 +0.6
4,196,528 + BWI76_RS20025 0.84 +0.5
4,196,762 + -0.1
4,196,811 + BWI76_RS20030 0.10 +0.7
4,196,846 + BWI76_RS20030 0.15 -0.5
4,197,056 - BWI76_RS20030 0.47 +0.6
4,197,156 - BWI76_RS20030 0.61 +0.4
4,197,233 + BWI76_RS20030 0.73 +3.0
4,197,527 + +0.8
4,197,603 - -0.3
4,197,610 + +0.3
4,197,613 - -1.6
4,197,705 + BWI76_RS20035 0.16 +0.9
4,197,852 + BWI76_RS20035 0.31 +0.5
4,197,852 + BWI76_RS20035 0.31 +0.3
4,198,263 + BWI76_RS20035 0.73 +1.0
4,198,393 + BWI76_RS20035 0.86 +0.5

Or see this region's nucleotide sequence