Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09735 and BWI76_RS09740 are separated by 51 nucleotides BWI76_RS09740 and BWI76_RS09745 are separated by 382 nucleotides
BWI76_RS09735: BWI76_RS09735 - formate acetyltransferase, at 2,039,827 to 2,042,109
_RS09735
BWI76_RS09740: BWI76_RS09740 - formate transporter FocA, at 2,042,161 to 2,043,018
_RS09740
BWI76_RS09745: BWI76_RS09745 - 30S ribosomal protein S12 methylthiotransferase accessory protein YcaO, at 2,043,401 to 2,045,161
_RS09745
Position (kb)
2042
2043
2044 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2041.439 kb on - strand, within BWI76_RS09735 at 2041.554 kb on - strand, within BWI76_RS09735 at 2042.081 kb on + strand at 2042.484 kb on + strand, within BWI76_RS09740 at 2042.584 kb on + strand, within BWI76_RS09740 at 2042.672 kb on - strand, within BWI76_RS09740 at 2042.754 kb on + strand, within BWI76_RS09740 at 2042.864 kb on - strand, within BWI76_RS09740 at 2042.884 kb on + strand, within BWI76_RS09740 at 2042.884 kb on + strand, within BWI76_RS09740 at 2042.884 kb on + strand, within BWI76_RS09740 at 2042.884 kb on + strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.892 kb on - strand, within BWI76_RS09740 at 2042.900 kb on - strand, within BWI76_RS09740 at 2042.902 kb on - strand, within BWI76_RS09740 at 2042.902 kb on - strand, within BWI76_RS09740 at 2042.910 kb on + strand, within BWI76_RS09740 at 2042.976 kb on + strand at 2042.978 kb on + strand at 2043.004 kb on + strand at 2043.018 kb on + strand at 2043.026 kb on - strand at 2043.028 kb on + strand at 2043.030 kb on - strand at 2043.030 kb on - strand at 2043.063 kb on + strand at 2043.063 kb on + strand at 2043.071 kb on - strand at 2043.071 kb on - strand at 2043.071 kb on - strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.088 kb on + strand at 2043.096 kb on - strand at 2043.096 kb on - strand at 2043.096 kb on - strand at 2043.096 kb on - strand at 2043.096 kb on - strand at 2043.097 kb on - strand at 2043.097 kb on - strand at 2043.121 kb on - strand at 2043.345 kb on + strand at 2043.447 kb on + strand at 2043.455 kb on - strand at 2043.530 kb on - strand at 2043.613 kb on - strand, within BWI76_RS09745 at 2043.635 kb on + strand, within BWI76_RS09745 at 2043.689 kb on + strand, within BWI76_RS09745 at 2043.711 kb on - strand, within BWI76_RS09745 at 2043.716 kb on - strand, within BWI76_RS09745 at 2043.778 kb on + strand, within BWI76_RS09745 at 2043.786 kb on - strand, within BWI76_RS09745
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 2,041,439 - BWI76_RS09735 0.71 -2.7 2,041,554 - BWI76_RS09735 0.76 -1.3 2,042,081 + -0.6 2,042,484 + BWI76_RS09740 0.38 -2.0 2,042,584 + BWI76_RS09740 0.49 -2.1 2,042,672 - BWI76_RS09740 0.60 -0.2 2,042,754 + BWI76_RS09740 0.69 +2.6 2,042,864 - BWI76_RS09740 0.82 -0.6 2,042,884 + BWI76_RS09740 0.84 -1.0 2,042,884 + BWI76_RS09740 0.84 -3.0 2,042,884 + BWI76_RS09740 0.84 -0.6 2,042,884 + BWI76_RS09740 0.84 -3.0 2,042,892 - BWI76_RS09740 0.85 -3.6 2,042,892 - BWI76_RS09740 0.85 -2.0 2,042,892 - BWI76_RS09740 0.85 -0.4 2,042,892 - BWI76_RS09740 0.85 -0.7 2,042,892 - BWI76_RS09740 0.85 -1.1 2,042,900 - BWI76_RS09740 0.86 -1.4 2,042,902 - BWI76_RS09740 0.86 -3.0 2,042,902 - BWI76_RS09740 0.86 -1.5 2,042,910 + BWI76_RS09740 0.87 -1.4 2,042,976 + -0.3 2,042,978 + -0.6 2,043,004 + +0.0 2,043,018 + -2.1 2,043,026 - -0.6 2,043,028 + -0.6 2,043,030 - -3.1 2,043,030 - -1.3 2,043,063 + +0.7 2,043,063 + +0.0 2,043,071 - -0.5 2,043,071 - +0.0 2,043,071 - -1.6 2,043,088 + -0.0 2,043,088 + -2.7 2,043,088 + -0.8 2,043,088 + +0.2 2,043,088 + +0.3 2,043,088 + +0.5 2,043,088 + +1.6 2,043,088 + -0.2 2,043,088 + -0.0 2,043,088 + +0.4 2,043,096 - +0.4 2,043,096 - -1.3 2,043,096 - +0.2 2,043,096 - -2.3 2,043,096 - +2.6 2,043,097 - -0.3 2,043,097 - +0.4 2,043,121 - +2.1 2,043,345 + +0.4 2,043,447 + -0.1 2,043,455 - +0.4 2,043,530 - +0.3 2,043,613 - BWI76_RS09745 0.12 -0.3 2,043,635 + BWI76_RS09745 0.13 +1.3 2,043,689 + BWI76_RS09745 0.16 -0.3 2,043,711 - BWI76_RS09745 0.18 -0.5 2,043,716 - BWI76_RS09745 0.18 -1.0 2,043,778 + BWI76_RS09745 0.21 -0.5 2,043,786 - BWI76_RS09745 0.22 -1.1
Or see this region's nucleotide sequence