Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10525 and BWI76_RS10530 are separated by 143 nucleotides BWI76_RS10530 and BWI76_RS10535 overlap by 1 nucleotides BWI76_RS10535 and BWI76_RS10540 are separated by 16 nucleotides
BWI76_RS10525: BWI76_RS10525 - cytosine/purines uracil thiamine allantoin permease, at 2,223,822 to 2,225,309
_RS10525
BWI76_RS10530: BWI76_RS10530 - GntR family transcriptional regulator with, at 2,225,453 to 2,226,169
_RS10530
BWI76_RS10535: BWI76_RS10535 - Asp/Glu/hydantoin racemase, at 2,226,169 to 2,226,912
_RS10535
BWI76_RS10540: BWI76_RS10540 - allantoinase, at 2,226,929 to 2,227,861
_RS10540
Position (kb)
2225
2226
2227 Strain fitness (log2 ratio)
-2
-1
0
1 at 2224.465 kb on - strand, within BWI76_RS10525 at 2224.654 kb on - strand, within BWI76_RS10525 at 2224.798 kb on - strand, within BWI76_RS10525 at 2225.061 kb on - strand, within BWI76_RS10525 at 2225.061 kb on - strand, within BWI76_RS10525 at 2225.151 kb on - strand, within BWI76_RS10525 at 2225.283 kb on - strand at 2225.358 kb on - strand at 2225.716 kb on - strand, within BWI76_RS10530 at 2225.939 kb on + strand, within BWI76_RS10530 at 2226.330 kb on - strand, within BWI76_RS10535 at 2226.357 kb on + strand, within BWI76_RS10535 at 2226.595 kb on - strand, within BWI76_RS10535 at 2226.975 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,224,465 - BWI76_RS10525 0.43 +0.4 2,224,654 - BWI76_RS10525 0.56 -0.1 2,224,798 - BWI76_RS10525 0.66 -0.7 2,225,061 - BWI76_RS10525 0.83 +0.2 2,225,061 - BWI76_RS10525 0.83 +0.4 2,225,151 - BWI76_RS10525 0.89 +0.5 2,225,283 - +0.5 2,225,358 - +0.5 2,225,716 - BWI76_RS10530 0.37 +1.0 2,225,939 + BWI76_RS10530 0.68 +0.8 2,226,330 - BWI76_RS10535 0.22 +0.8 2,226,357 + BWI76_RS10535 0.25 +1.0 2,226,595 - BWI76_RS10535 0.57 +0.4 2,226,975 + -2.1
Or see this region's nucleotide sequence