Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26915 and BWI76_RS26920 are separated by 159 nucleotides BWI76_RS26920 and BWI76_RS26925 overlap by 23 nucleotides BWI76_RS26925 and BWI76_RS26930 overlap by 38 nucleotides
BWI76_RS26915: BWI76_RS26915 - hypothetical protein, at 5,583,536 to 5,584,180
_RS26915
BWI76_RS26920: BWI76_RS26920 - DNA-3-methyladenine glycosylase I, at 5,584,340 to 5,584,921
_RS26920
BWI76_RS26925: BWI76_RS26925 - N-acetyltransferase, at 5,584,899 to 5,585,345
_RS26925
BWI76_RS26930: BWI76_RS26930 - biotin sulfoxide reductase, at 5,585,308 to 5,587,638
_RS26930
Position (kb) 5584
5585
5586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5584.018 kb on + strand, within BWI76_RS26915 at 5584.028 kb on + strand, within BWI76_RS26915 at 5584.031 kb on - strand, within BWI76_RS26915 at 5584.036 kb on - strand, within BWI76_RS26915 at 5584.038 kb on - strand, within BWI76_RS26915 at 5584.043 kb on - strand, within BWI76_RS26915 at 5584.050 kb on + strand, within BWI76_RS26915 at 5584.050 kb on + strand, within BWI76_RS26915 at 5584.082 kb on - strand, within BWI76_RS26915 at 5584.084 kb on + strand, within BWI76_RS26915 at 5584.116 kb on + strand at 5584.149 kb on + strand at 5584.208 kb on - strand at 5584.258 kb on + strand at 5584.329 kb on + strand at 5584.378 kb on - strand at 5584.378 kb on - strand at 5584.515 kb on + strand, within BWI76_RS26920 at 5584.533 kb on - strand, within BWI76_RS26920 at 5584.571 kb on + strand, within BWI76_RS26920 at 5584.830 kb on + strand, within BWI76_RS26920 at 5584.830 kb on + strand, within BWI76_RS26920 at 5584.840 kb on + strand, within BWI76_RS26920 at 5584.848 kb on - strand, within BWI76_RS26920 at 5584.852 kb on + strand, within BWI76_RS26920 at 5584.900 kb on - strand at 5585.003 kb on - strand, within BWI76_RS26925 at 5585.097 kb on - strand, within BWI76_RS26925 at 5585.165 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.200 kb on + strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.208 kb on - strand, within BWI76_RS26925 at 5585.244 kb on - strand, within BWI76_RS26925 at 5585.255 kb on - strand, within BWI76_RS26925 at 5585.312 kb on + strand at 5585.475 kb on + strand at 5585.625 kb on + strand, within BWI76_RS26930 at 5585.633 kb on - strand, within BWI76_RS26930 at 5585.726 kb on + strand, within BWI76_RS26930 at 5585.747 kb on - strand, within BWI76_RS26930
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,584,018 + BWI76_RS26915 0.75 +0.3 5,584,028 + BWI76_RS26915 0.76 +0.9 5,584,031 - BWI76_RS26915 0.77 -0.4 5,584,036 - BWI76_RS26915 0.78 -0.3 5,584,038 - BWI76_RS26915 0.78 -0.8 5,584,043 - BWI76_RS26915 0.79 +1.5 5,584,050 + BWI76_RS26915 0.80 -1.2 5,584,050 + BWI76_RS26915 0.80 +0.1 5,584,082 - BWI76_RS26915 0.85 +0.2 5,584,084 + BWI76_RS26915 0.85 -1.9 5,584,116 + +0.4 5,584,149 + -0.5 5,584,208 - +0.1 5,584,258 + +0.0 5,584,329 + -0.6 5,584,378 - -0.5 5,584,378 - -2.8 5,584,515 + BWI76_RS26920 0.30 +0.8 5,584,533 - BWI76_RS26920 0.33 -0.5 5,584,571 + BWI76_RS26920 0.40 +0.3 5,584,830 + BWI76_RS26920 0.84 +0.0 5,584,830 + BWI76_RS26920 0.84 -0.6 5,584,840 + BWI76_RS26920 0.86 +0.7 5,584,848 - BWI76_RS26920 0.87 -1.5 5,584,852 + BWI76_RS26920 0.88 -0.7 5,584,900 - -0.7 5,585,003 - BWI76_RS26925 0.23 +0.0 5,585,097 - BWI76_RS26925 0.44 +0.5 5,585,165 + BWI76_RS26925 0.60 +1.3 5,585,200 + -0.2 5,585,200 + BWI76_RS26925 0.67 -0.3 5,585,200 + BWI76_RS26925 0.67 +0.7 5,585,200 + BWI76_RS26925 0.67 -0.5 5,585,200 + BWI76_RS26925 0.67 +2.1 5,585,200 + BWI76_RS26925 0.67 +0.1 5,585,208 - BWI76_RS26925 0.69 +0.0 5,585,208 - BWI76_RS26925 0.69 +0.1 5,585,208 - BWI76_RS26925 0.69 +1.6 5,585,208 - BWI76_RS26925 0.69 +0.9 5,585,208 - BWI76_RS26925 0.69 -2.0 5,585,208 - BWI76_RS26925 0.69 +1.5 5,585,208 - BWI76_RS26925 0.69 +1.6 5,585,244 - BWI76_RS26925 0.77 -1.0 5,585,255 - BWI76_RS26925 0.80 +0.8 5,585,312 + +0.6 5,585,475 + +0.1 5,585,625 + BWI76_RS26930 0.14 +0.8 5,585,633 - BWI76_RS26930 0.14 +0.5 5,585,726 + BWI76_RS26930 0.18 -1.1 5,585,747 - BWI76_RS26930 0.19 +0.6
Or see this region's nucleotide sequence