Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01920 and BWI76_RS01925 are separated by 214 nucleotides BWI76_RS01925 and BWI76_RS01930 are separated by 197 nucleotides
BWI76_RS01920: BWI76_RS01920 - alanine racemase, at 391,002 to 392,081
_RS01920
BWI76_RS01925: BWI76_RS01925 - aromatic amino acid aminotransferase, at 392,296 to 393,489
_RS01925
BWI76_RS01930: BWI76_RS01930 - acid phosphatase/phosphotransferase, at 393,687 to 394,400
_RS01930
Position (kb) 392
393
394 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 391.301 kb on - strand, within BWI76_RS01920 at 391.301 kb on - strand, within BWI76_RS01920 at 391.417 kb on + strand, within BWI76_RS01920 at 391.429 kb on + strand, within BWI76_RS01920 at 391.439 kb on + strand, within BWI76_RS01920 at 391.439 kb on + strand, within BWI76_RS01920 at 391.447 kb on - strand, within BWI76_RS01920 at 391.555 kb on + strand, within BWI76_RS01920 at 391.590 kb on + strand, within BWI76_RS01920 at 391.830 kb on + strand, within BWI76_RS01920 at 392.257 kb on - strand at 392.267 kb on - strand at 392.508 kb on + strand, within BWI76_RS01925 at 392.563 kb on - strand, within BWI76_RS01925 at 392.596 kb on - strand, within BWI76_RS01925 at 392.840 kb on + strand, within BWI76_RS01925 at 392.860 kb on + strand, within BWI76_RS01925 at 392.988 kb on - strand, within BWI76_RS01925 at 393.187 kb on + strand, within BWI76_RS01925 at 393.212 kb on - strand, within BWI76_RS01925 at 393.263 kb on + strand, within BWI76_RS01925 at 393.273 kb on + strand, within BWI76_RS01925 at 393.280 kb on - strand, within BWI76_RS01925 at 393.281 kb on + strand, within BWI76_RS01925 at 393.369 kb on + strand, within BWI76_RS01925 at 393.371 kb on - strand at 393.371 kb on - strand at 393.371 kb on - strand at 393.390 kb on + strand at 393.457 kb on - strand at 393.457 kb on - strand at 393.463 kb on + strand at 393.484 kb on + strand at 393.493 kb on - strand at 393.504 kb on + strand at 393.512 kb on - strand at 393.531 kb on + strand at 393.531 kb on + strand at 393.531 kb on + strand at 393.539 kb on - strand at 393.583 kb on - strand at 393.699 kb on + strand at 393.706 kb on + strand at 393.823 kb on - strand, within BWI76_RS01930 at 393.823 kb on - strand, within BWI76_RS01930 at 393.838 kb on - strand, within BWI76_RS01930 at 394.227 kb on + strand, within BWI76_RS01930 at 394.285 kb on + strand, within BWI76_RS01930 at 394.369 kb on + strand at 394.377 kb on - strand at 394.473 kb on + strand at 394.488 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 391,301 - BWI76_RS01920 0.28 -2.5 391,301 - BWI76_RS01920 0.28 +1.3 391,417 + BWI76_RS01920 0.38 -0.0 391,429 + BWI76_RS01920 0.40 -0.0 391,439 + BWI76_RS01920 0.40 +0.4 391,439 + BWI76_RS01920 0.40 -2.3 391,447 - BWI76_RS01920 0.41 +1.2 391,555 + BWI76_RS01920 0.51 +0.6 391,590 + BWI76_RS01920 0.54 -0.8 391,830 + BWI76_RS01920 0.77 -0.8 392,257 - -0.1 392,267 - +0.2 392,508 + BWI76_RS01925 0.18 -0.8 392,563 - BWI76_RS01925 0.22 -1.3 392,596 - BWI76_RS01925 0.25 +1.0 392,840 + BWI76_RS01925 0.46 -2.2 392,860 + BWI76_RS01925 0.47 +0.1 392,988 - BWI76_RS01925 0.58 -1.9 393,187 + BWI76_RS01925 0.75 -0.4 393,212 - BWI76_RS01925 0.77 -0.8 393,263 + BWI76_RS01925 0.81 -0.4 393,273 + BWI76_RS01925 0.82 -1.3 393,280 - BWI76_RS01925 0.82 -2.1 393,281 + BWI76_RS01925 0.82 +0.8 393,369 + BWI76_RS01925 0.90 +0.1 393,371 - -2.7 393,371 - +0.7 393,371 - -2.5 393,390 + -2.7 393,457 - -1.2 393,457 - -3.8 393,463 + -0.1 393,484 + +0.0 393,493 - -0.3 393,504 + +0.6 393,512 - +0.1 393,531 + +0.4 393,531 + +0.2 393,531 + -0.6 393,539 - +0.3 393,583 - +0.8 393,699 + -1.8 393,706 + +1.2 393,823 - BWI76_RS01930 0.19 +0.2 393,823 - BWI76_RS01930 0.19 +0.7 393,838 - BWI76_RS01930 0.21 +0.0 394,227 + BWI76_RS01930 0.76 -1.1 394,285 + BWI76_RS01930 0.84 +0.1 394,369 + +0.4 394,377 - -0.8 394,473 + -1.0 394,488 - +0.4
Or see this region's nucleotide sequence