Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17365 and BWI76_RS17370 are separated by 9 nucleotides BWI76_RS17370 and BWI76_RS17375 are separated by 10 nucleotides BWI76_RS17375 and BWI76_RS17380 are separated by 1 nucleotides
BWI76_RS17365: BWI76_RS17365 - Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (from data) , at 3,600,666 to 3,604,736
_RS17365
BWI76_RS17370: BWI76_RS17370 - nitrate transporter NasD, at 3,604,746 to 3,605,534
_RS17370
BWI76_RS17375: BWI76_RS17375 - nitrate ABC transporter, permease protein, at 3,605,545 to 3,606,429
_RS17375
BWI76_RS17380: BWI76_RS17380 - nitrate transporter component, at 3,606,431 to 3,607,687
_RS17380
Position (kb)
3604
3605
3606 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3603.750 kb on - strand, within BWI76_RS17365 at 3604.045 kb on - strand, within BWI76_RS17365 at 3604.315 kb on - strand, within BWI76_RS17365 at 3604.466 kb on - strand at 3604.807 kb on - strand at 3605.341 kb on + strand, within BWI76_RS17370 at 3605.452 kb on - strand, within BWI76_RS17370 at 3605.877 kb on + strand, within BWI76_RS17375 at 3606.323 kb on - strand, within BWI76_RS17375
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,603,750 - BWI76_RS17365 0.76 +0.3 3,604,045 - BWI76_RS17365 0.83 -0.2 3,604,315 - BWI76_RS17365 0.90 -3.7 3,604,466 - +1.1 3,604,807 - +0.4 3,605,341 + BWI76_RS17370 0.75 -1.7 3,605,452 - BWI76_RS17370 0.89 -1.2 3,605,877 + BWI76_RS17375 0.38 +1.1 3,606,323 - BWI76_RS17375 0.88 +0.0
Or see this region's nucleotide sequence