Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07855 and BWI76_RS07860 are separated by 2 nucleotides BWI76_RS07860 and BWI76_RS07865 are separated by 504 nucleotides
BWI76_RS07855: BWI76_RS07855 - PucR family transcriptional regulator, at 1,666,385 to 1,667,620
_RS07855
BWI76_RS07860: BWI76_RS07860 - cytosine permease, at 1,667,623 to 1,668,885
_RS07860
BWI76_RS07865: BWI76_RS07865 - methylthioribulose 1-phosphate dehydratase, at 1,669,390 to 1,670,001
_RS07865
Position (kb)
1667
1668
1669 Strain fitness (log2 ratio)
-2
-1
0
1 at 1666.669 kb on + strand at 1667.291 kb on - strand, within BWI76_RS07855 at 1667.622 kb on - strand at 1667.700 kb on + strand at 1667.700 kb on + strand at 1667.700 kb on + strand at 1667.887 kb on - strand, within BWI76_RS07860 at 1668.228 kb on + strand, within BWI76_RS07860 at 1668.508 kb on - strand at 1668.903 kb on - strand at 1668.931 kb on + strand at 1668.962 kb on + strand at 1668.984 kb on - strand at 1669.233 kb on - strand at 1669.326 kb on + strand at 1669.484 kb on + strand, within BWI76_RS07865 at 1669.568 kb on - strand, within BWI76_RS07865 at 1669.731 kb on + strand, within BWI76_RS07865
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,666,669 + +1.0 1,667,291 - BWI76_RS07855 0.73 -0.0 1,667,622 - +0.4 1,667,700 + +0.6 1,667,700 + +0.7 1,667,700 + +0.2 1,667,887 - BWI76_RS07860 0.21 +0.2 1,668,228 + BWI76_RS07860 0.48 +1.2 1,668,508 - -1.3 1,668,903 - -0.2 1,668,931 + -2.0 1,668,962 + -0.3 1,668,984 - +0.4 1,669,233 - -0.2 1,669,326 + +0.4 1,669,484 + BWI76_RS07865 0.15 -1.0 1,669,568 - BWI76_RS07865 0.29 +0.6 1,669,731 + BWI76_RS07865 0.56 -0.2
Or see this region's nucleotide sequence