Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02520 and BWI76_RS02525 are separated by 10 nucleotides BWI76_RS02525 and BWI76_RS02530 overlap by 8 nucleotides
BWI76_RS02520: BWI76_RS02520 - N-acetylmuramoyl-L-alanine amidase, at 515,678 to 517,015
_RS02520
BWI76_RS02525: BWI76_RS02525 - DNA mismatch repair protein MutL, at 517,026 to 518,921
_RS02525
BWI76_RS02530: BWI76_RS02530 - tRNA dimethylallyltransferase, at 518,914 to 519,864
_RS02530
Position (kb) 517
518
519 Strain fitness (log2 ratio) -2
-1
0
1
2 at 516.171 kb on - strand, within BWI76_RS02520 at 516.196 kb on + strand, within BWI76_RS02520 at 516.204 kb on - strand, within BWI76_RS02520 at 516.367 kb on + strand, within BWI76_RS02520 at 516.573 kb on + strand, within BWI76_RS02520 at 516.610 kb on - strand, within BWI76_RS02520 at 516.702 kb on + strand, within BWI76_RS02520 at 516.739 kb on + strand, within BWI76_RS02520 at 516.810 kb on + strand, within BWI76_RS02520 at 517.279 kb on + strand, within BWI76_RS02525 at 517.279 kb on + strand, within BWI76_RS02525 at 517.287 kb on - strand, within BWI76_RS02525 at 517.317 kb on + strand, within BWI76_RS02525 at 517.317 kb on + strand, within BWI76_RS02525 at 517.325 kb on - strand, within BWI76_RS02525 at 517.358 kb on - strand, within BWI76_RS02525 at 517.464 kb on - strand, within BWI76_RS02525 at 517.790 kb on + strand, within BWI76_RS02525 at 517.893 kb on - strand, within BWI76_RS02525 at 517.926 kb on - strand, within BWI76_RS02525 at 518.074 kb on - strand, within BWI76_RS02525 at 518.152 kb on + strand, within BWI76_RS02525 at 518.287 kb on + strand, within BWI76_RS02525 at 518.325 kb on + strand, within BWI76_RS02525 at 518.468 kb on - strand, within BWI76_RS02525 at 518.502 kb on + strand, within BWI76_RS02525 at 518.512 kb on + strand, within BWI76_RS02525 at 518.621 kb on - strand, within BWI76_RS02525 at 518.651 kb on + strand, within BWI76_RS02525 at 518.652 kb on - strand, within BWI76_RS02525 at 518.659 kb on - strand, within BWI76_RS02525 at 518.758 kb on + strand at 518.816 kb on + strand at 518.846 kb on + strand at 518.854 kb on - strand at 518.854 kb on - strand at 518.940 kb on + strand at 519.180 kb on + strand, within BWI76_RS02530 at 519.287 kb on - strand, within BWI76_RS02530 at 519.287 kb on - strand, within BWI76_RS02530 at 519.305 kb on - strand, within BWI76_RS02530 at 519.530 kb on + strand, within BWI76_RS02530 at 519.776 kb on + strand at 519.853 kb on + strand at 519.882 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 516,171 - BWI76_RS02520 0.37 -0.6 516,196 + BWI76_RS02520 0.39 -1.0 516,204 - BWI76_RS02520 0.39 -0.4 516,367 + BWI76_RS02520 0.51 +0.4 516,573 + BWI76_RS02520 0.67 +0.1 516,610 - BWI76_RS02520 0.70 -0.7 516,702 + BWI76_RS02520 0.77 +0.3 516,739 + BWI76_RS02520 0.79 -0.7 516,810 + BWI76_RS02520 0.85 +0.3 517,279 + BWI76_RS02525 0.13 -0.8 517,279 + BWI76_RS02525 0.13 -1.7 517,287 - BWI76_RS02525 0.14 -0.7 517,317 + BWI76_RS02525 0.15 -2.4 517,317 + BWI76_RS02525 0.15 +0.1 517,325 - BWI76_RS02525 0.16 -1.1 517,358 - BWI76_RS02525 0.18 -1.7 517,464 - BWI76_RS02525 0.23 -2.1 517,790 + BWI76_RS02525 0.40 +0.5 517,893 - BWI76_RS02525 0.46 -1.0 517,926 - BWI76_RS02525 0.47 -1.0 518,074 - BWI76_RS02525 0.55 +0.0 518,152 + BWI76_RS02525 0.59 -0.1 518,287 + BWI76_RS02525 0.67 +0.4 518,325 + BWI76_RS02525 0.69 +0.4 518,468 - BWI76_RS02525 0.76 -2.7 518,502 + BWI76_RS02525 0.78 -0.3 518,512 + BWI76_RS02525 0.78 -0.1 518,621 - BWI76_RS02525 0.84 -0.8 518,651 + BWI76_RS02525 0.86 -1.2 518,652 - BWI76_RS02525 0.86 +2.3 518,659 - BWI76_RS02525 0.86 -2.1 518,758 + -0.0 518,816 + +0.3 518,846 + -0.0 518,854 - +0.3 518,854 - -2.3 518,940 + +1.3 519,180 + BWI76_RS02530 0.28 -0.3 519,287 - BWI76_RS02530 0.39 +1.3 519,287 - BWI76_RS02530 0.39 +1.3 519,305 - BWI76_RS02530 0.41 -1.1 519,530 + BWI76_RS02530 0.65 -1.6 519,776 + -1.9 519,853 + -0.6 519,882 + +0.6
Or see this region's nucleotide sequence