Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2284

Experiment: KB with Harmalol 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2283 and Psyr_2284 are separated by 138 nucleotidesPsyr_2284 and Psyr_2285 are separated by 174 nucleotidesPsyr_2285 and Psyr_2286 overlap by 7 nucleotides Psyr_2283: Psyr_2283 - Secretion protein HlyD, at 2,653,659 to 2,654,819 _2283 Psyr_2284: Psyr_2284 - transcriptional regulator, LacI family, at 2,654,958 to 2,655,548 _2284 Psyr_2285: Psyr_2285 - Twin-arginine translocation pathway signal, at 2,655,723 to 2,656,328 _2285 Psyr_2286: Psyr_2286 - Protein of unknown function DUF461, at 2,656,322 to 2,656,816 _2286 Position (kb) 2654 2655 2656Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2654.062 kb on + strand, within Psyr_2283at 2654.063 kb on - strand, within Psyr_2283at 2654.128 kb on - strand, within Psyr_2283at 2654.179 kb on + strand, within Psyr_2283at 2654.299 kb on + strand, within Psyr_2283at 2654.432 kb on - strand, within Psyr_2283at 2654.781 kb on + strandat 2654.781 kb on + strandat 2654.813 kb on + strandat 2654.813 kb on + strandat 2654.814 kb on - strandat 2654.849 kb on - strandat 2654.924 kb on - strandat 2654.926 kb on - strandat 2654.976 kb on - strandat 2655.004 kb on + strandat 2655.004 kb on + strandat 2655.004 kb on + strandat 2655.005 kb on - strandat 2655.578 kb on + strandat 2655.579 kb on - strandat 2655.579 kb on - strandat 2655.662 kb on + strandat 2655.663 kb on - strandat 2656.201 kb on - strand, within Psyr_2285at 2656.201 kb on - strand, within Psyr_2285at 2656.201 kb on - strand, within Psyr_2285at 2656.227 kb on + strand, within Psyr_2285at 2656.228 kb on - strand, within Psyr_2285at 2656.321 kb on + strandat 2656.321 kb on + strandat 2656.321 kb on + strandat 2656.321 kb on + strandat 2656.322 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Harmalol 10 mM
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2,654,062 + Psyr_2283 0.35 -3.1
2,654,063 - Psyr_2283 0.35 -0.7
2,654,128 - Psyr_2283 0.40 -1.1
2,654,179 + Psyr_2283 0.45 +0.9
2,654,299 + Psyr_2283 0.55 -2.1
2,654,432 - Psyr_2283 0.67 -3.3
2,654,781 + -2.1
2,654,781 + -2.7
2,654,813 + -3.2
2,654,813 + -0.7
2,654,814 - -2.7
2,654,849 - -0.3
2,654,924 - +0.6
2,654,926 - +0.3
2,654,976 - +0.2
2,655,004 + +0.8
2,655,004 + +0.7
2,655,004 + -0.1
2,655,005 - +1.8
2,655,578 + +0.2
2,655,579 - -0.1
2,655,579 - -0.5
2,655,662 + -0.4
2,655,663 - -1.5
2,656,201 - Psyr_2285 0.79 +1.7
2,656,201 - Psyr_2285 0.79 +0.7
2,656,201 - Psyr_2285 0.79 -0.8
2,656,227 + Psyr_2285 0.83 +1.7
2,656,228 - Psyr_2285 0.83 +2.5
2,656,321 + -0.1
2,656,321 + -2.8
2,656,321 + -0.8
2,656,321 + -0.3
2,656,322 - -2.0

Or see this region's nucleotide sequence