Experiment: RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01590 and BWI76_RS01595 are separated by 12 nucleotides BWI76_RS01595 and BWI76_RS01600 are separated by 251 nucleotides BWI76_RS01600 and BWI76_RS01605 overlap by 4 nucleotides
BWI76_RS01590: BWI76_RS01590 - hypothetical protein, at 322,429 to 323,121
_RS01590
BWI76_RS01595: BWI76_RS01595 - zinc resistance protein, at 323,134 to 323,556
_RS01595
BWI76_RS01600: BWI76_RS01600 - sensor protein ZraS, at 323,808 to 325,196
_RS01600
BWI76_RS01605: BWI76_RS01605 - transcriptional regulatory protein ZraR, at 325,193 to 326,524
_RS01605
Position (kb) 323
324
325
326 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 322.952 kb on + strand, within BWI76_RS01590 at 322.973 kb on - strand, within BWI76_RS01590 at 323.025 kb on - strand, within BWI76_RS01590 at 323.054 kb on + strand at 323.085 kb on + strand at 323.085 kb on - strand at 323.173 kb on + strand at 323.173 kb on + strand at 323.181 kb on - strand, within BWI76_RS01595 at 323.428 kb on - strand, within BWI76_RS01595 at 323.432 kb on + strand, within BWI76_RS01595 at 323.461 kb on - strand, within BWI76_RS01595 at 323.606 kb on - strand at 323.783 kb on - strand at 323.785 kb on - strand at 323.785 kb on - strand at 323.785 kb on - strand at 323.869 kb on + strand at 323.877 kb on - strand at 323.902 kb on + strand at 324.314 kb on + strand, within BWI76_RS01600 at 324.447 kb on + strand, within BWI76_RS01600 at 324.455 kb on - strand, within BWI76_RS01600 at 324.460 kb on - strand, within BWI76_RS01600 at 324.601 kb on - strand, within BWI76_RS01600 at 324.645 kb on + strand, within BWI76_RS01600 at 324.652 kb on - strand, within BWI76_RS01600 at 324.908 kb on - strand, within BWI76_RS01600 at 324.934 kb on - strand, within BWI76_RS01600 at 324.992 kb on + strand, within BWI76_RS01600 at 325.062 kb on - strand at 325.095 kb on - strand at 325.250 kb on + strand at 325.329 kb on + strand, within BWI76_RS01605 at 325.435 kb on + strand, within BWI76_RS01605 at 325.443 kb on - strand, within BWI76_RS01605 at 325.450 kb on + strand, within BWI76_RS01605 at 325.453 kb on - strand, within BWI76_RS01605 at 325.495 kb on + strand, within BWI76_RS01605 at 325.528 kb on + strand, within BWI76_RS01605 at 325.606 kb on + strand, within BWI76_RS01605 at 325.643 kb on + strand, within BWI76_RS01605 at 325.736 kb on - strand, within BWI76_RS01605 at 325.812 kb on + strand, within BWI76_RS01605 at 325.901 kb on - strand, within BWI76_RS01605 at 325.976 kb on + strand, within BWI76_RS01605 at 326.186 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Burkholderia bryophila 376MFSha3.1 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 322,952 + BWI76_RS01590 0.75 -2.0 322,973 - BWI76_RS01590 0.78 -1.9 323,025 - BWI76_RS01590 0.86 +0.8 323,054 + -1.9 323,085 + -0.5 323,085 - +0.1 323,173 + +1.1 323,173 + +0.2 323,181 - BWI76_RS01595 0.11 -2.3 323,428 - BWI76_RS01595 0.70 +0.9 323,432 + BWI76_RS01595 0.70 +0.1 323,461 - BWI76_RS01595 0.77 +0.1 323,606 - +0.3 323,783 - +0.1 323,785 - +1.1 323,785 - +0.9 323,785 - -0.9 323,869 + +1.1 323,877 - -1.9 323,902 + -0.3 324,314 + BWI76_RS01600 0.36 +0.6 324,447 + BWI76_RS01600 0.46 -0.9 324,455 - BWI76_RS01600 0.47 +0.4 324,460 - BWI76_RS01600 0.47 +2.0 324,601 - BWI76_RS01600 0.57 +0.1 324,645 + BWI76_RS01600 0.60 -0.0 324,652 - BWI76_RS01600 0.61 +1.3 324,908 - BWI76_RS01600 0.79 +0.1 324,934 - BWI76_RS01600 0.81 +1.4 324,992 + BWI76_RS01600 0.85 +1.4 325,062 - +0.7 325,095 - +0.4 325,250 + -1.7 325,329 + BWI76_RS01605 0.10 +0.4 325,435 + BWI76_RS01605 0.18 +0.4 325,443 - BWI76_RS01605 0.19 -2.8 325,450 + BWI76_RS01605 0.19 -0.0 325,453 - BWI76_RS01605 0.20 -0.1 325,495 + BWI76_RS01605 0.23 +0.6 325,528 + BWI76_RS01605 0.25 +0.2 325,606 + BWI76_RS01605 0.31 +2.0 325,643 + BWI76_RS01605 0.34 -0.2 325,736 - BWI76_RS01605 0.41 +0.5 325,812 + BWI76_RS01605 0.46 -0.1 325,901 - BWI76_RS01605 0.53 -0.2 325,976 + BWI76_RS01605 0.59 -0.3 326,186 - +1.1
Or see this region's nucleotide sequence