Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26060 and BWI76_RS26065 are separated by 3 nucleotides BWI76_RS26065 and BWI76_RS26070 overlap by 8 nucleotides BWI76_RS26070 and BWI76_RS26075 are separated by 17 nucleotides
BWI76_RS26060: BWI76_RS26060 - tryptophan--tRNA ligase, at 5,385,429 to 5,386,433
_RS26060
BWI76_RS26065: BWI76_RS26065 - phosphoglycolate phosphatase, at 5,386,437 to 5,387,186
_RS26065
BWI76_RS26070: BWI76_RS26070 - ribulose-phosphate 3-epimerase, at 5,387,179 to 5,387,856
_RS26070
BWI76_RS26075: BWI76_RS26075 - DNA adenine methylase, at 5,387,874 to 5,388,701
_RS26075
Position (kb) 5386
5387
5388 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5385.440 kb on - strand at 5385.542 kb on - strand, within BWI76_RS26060 at 5385.542 kb on - strand, within BWI76_RS26060 at 5385.564 kb on + strand, within BWI76_RS26060 at 5385.610 kb on + strand, within BWI76_RS26060 at 5385.706 kb on - strand, within BWI76_RS26060 at 5385.867 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.074 kb on - strand, within BWI76_RS26060 at 5386.190 kb on + strand, within BWI76_RS26060 at 5386.236 kb on - strand, within BWI76_RS26060 at 5386.258 kb on + strand, within BWI76_RS26060 at 5386.266 kb on - strand, within BWI76_RS26060 at 5386.266 kb on - strand, within BWI76_RS26060 at 5386.393 kb on + strand at 5386.393 kb on + strand at 5386.401 kb on - strand at 5386.542 kb on - strand, within BWI76_RS26065 at 5386.657 kb on + strand, within BWI76_RS26065 at 5386.746 kb on + strand, within BWI76_RS26065 at 5386.993 kb on + strand, within BWI76_RS26065 at 5387.143 kb on - strand at 5387.194 kb on - strand at 5387.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,385,440 - -2.5 5,385,542 - BWI76_RS26060 0.11 +0.3 5,385,542 - BWI76_RS26060 0.11 -0.6 5,385,564 + BWI76_RS26060 0.13 -0.3 5,385,610 + BWI76_RS26060 0.18 +2.2 5,385,706 - BWI76_RS26060 0.28 +0.4 5,385,867 - BWI76_RS26060 0.44 +1.2 5,386,074 - BWI76_RS26060 0.64 -0.4 5,386,074 - BWI76_RS26060 0.64 +0.8 5,386,074 - BWI76_RS26060 0.64 +0.2 5,386,190 + BWI76_RS26060 0.76 +1.2 5,386,236 - BWI76_RS26060 0.80 +1.9 5,386,258 + BWI76_RS26060 0.82 +0.0 5,386,266 - BWI76_RS26060 0.83 -0.6 5,386,266 - BWI76_RS26060 0.83 -0.8 5,386,393 + -1.4 5,386,393 + -1.7 5,386,401 - +0.2 5,386,542 - BWI76_RS26065 0.14 +0.2 5,386,657 + BWI76_RS26065 0.29 -3.2 5,386,746 + BWI76_RS26065 0.41 -1.1 5,386,993 + BWI76_RS26065 0.74 -2.4 5,387,143 - +2.8 5,387,194 - -1.4 5,387,903 - +0.2
Or see this region's nucleotide sequence