Experiment: Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27315 and BWI76_RS27320 overlap by 1 nucleotides BWI76_RS27320 and BWI76_RS27325 overlap by 4 nucleotides BWI76_RS27325 and BWI76_RS27330 are separated by 100 nucleotides BWI76_RS27330 and BWI76_RS27335 are separated by 63 nucleotides
BWI76_RS27315: BWI76_RS27315 - 3-deoxy-D-manno-octulosonic acid transferase, at 5,669,571 to 5,670,845
_RS27315
BWI76_RS27320: BWI76_RS27320 - glucosyl transferase, at 5,670,845 to 5,671,621
_RS27320
BWI76_RS27325: BWI76_RS27325 - pantetheine-phosphate adenylyltransferase, at 5,671,618 to 5,672,094
_RS27325
BWI76_RS27330: BWI76_RS27330 - DNA-formamidopyrimidine glycosylase, at 5,672,195 to 5,673,004
_RS27330
BWI76_RS27335: BWI76_RS27335 - hypothetical protein, at 5,673,068 to 5,675,404
_RS27335
Position (kb)
5671
5672
5673 Strain fitness (log2 ratio)
-1
0
1 at 5672.233 kb on + strand at 5672.241 kb on - strand at 5672.371 kb on + strand, within BWI76_RS27330 at 5672.540 kb on - strand, within BWI76_RS27330 at 5672.586 kb on + strand, within BWI76_RS27330 at 5672.688 kb on + strand, within BWI76_RS27330 at 5672.752 kb on + strand, within BWI76_RS27330 at 5672.911 kb on - strand, within BWI76_RS27330 at 5672.967 kb on - strand at 5673.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with nitrate 20 mM remove 5,672,233 + +0.8 5,672,241 - -0.3 5,672,371 + BWI76_RS27330 0.22 +0.2 5,672,540 - BWI76_RS27330 0.43 +0.3 5,672,586 + BWI76_RS27330 0.48 -0.2 5,672,688 + BWI76_RS27330 0.61 +0.1 5,672,752 + BWI76_RS27330 0.69 -0.3 5,672,911 - BWI76_RS27330 0.88 +0.4 5,672,967 - -0.2 5,673,055 - +0.4
Or see this region's nucleotide sequence