Experiment: Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04495 and BWI76_RS04500 are separated by 84 nucleotides BWI76_RS04500 and BWI76_RS04505 overlap by 1 nucleotides BWI76_RS04505 and BWI76_RS04510 are separated by 40 nucleotides BWI76_RS04510 and BWI76_RS04515 are separated by 4 nucleotides
BWI76_RS04495: BWI76_RS04495 - glutathione-regulated potassium-efflux system protein KefC, at 930,808 to 932,673
_RS04495
BWI76_RS04500: BWI76_RS04500 - dihydrofolate reductase, at 932,758 to 932,871
_RS04500
BWI76_RS04505: BWI76_RS04505 - dihydrofolate reductase, at 932,871 to 933,350
_RS04505
BWI76_RS04510: BWI76_RS04510 - bis(5'-nucleosyl)-tetraphosphatase (symmetrical), at 933,391 to 934,239
_RS04510
BWI76_RS04515: BWI76_RS04515 - Co2+/Mg2+ efflux protein ApaG, at 934,244 to 934,621
_RS04515
Position (kb) 932
933
934 Strain fitness (log2 ratio) -2
-1
0
1 at 932.119 kb on - strand, within BWI76_RS04495 at 932.162 kb on + strand, within BWI76_RS04495 at 932.315 kb on + strand, within BWI76_RS04495 at 932.344 kb on - strand, within BWI76_RS04495 at 932.486 kb on - strand, within BWI76_RS04495 at 932.600 kb on + strand at 932.655 kb on + strand at 932.674 kb on + strand at 932.750 kb on + strand at 933.478 kb on + strand, within BWI76_RS04510 at 933.624 kb on + strand, within BWI76_RS04510 at 934.061 kb on - strand, within BWI76_RS04510 at 934.344 kb on - strand, within BWI76_RS04515
Per-strain Table
Position Strand Gene LocusTag Fraction Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x remove 932,119 - BWI76_RS04495 0.70 +0.2 932,162 + BWI76_RS04495 0.73 +1.6 932,315 + BWI76_RS04495 0.81 -0.8 932,344 - BWI76_RS04495 0.82 +0.1 932,486 - BWI76_RS04495 0.90 +0.7 932,600 + +0.2 932,655 + +0.1 932,674 + +0.6 932,750 + -1.8 933,478 + BWI76_RS04510 0.10 +1.0 933,624 + BWI76_RS04510 0.27 -0.6 934,061 - BWI76_RS04510 0.79 +1.0 934,344 - BWI76_RS04515 0.26 -1.0
Or see this region's nucleotide sequence