Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT4214

Experiment: BHIS with Simvastatin 30 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT4213 and BT4214 are separated by 72 nucleotidesBT4214 and BT4215 are separated by 152 nucleotidesBT4215 and BT4216 are separated by 38 nucleotides BT4213: BT4213 - conserved hypothetical protein (NCBI ptt file), at 5,550,093 to 5,550,995 BT4213 BT4214: BT4214 - conserved hypothetical protein (NCBI ptt file), at 5,551,068 to 5,551,940 BT4214 BT4215: BT4215 - shikimate dehydrogenase (EC 1.1.1.25) (from data), at 5,552,093 to 5,552,839 BT4215 BT4216: BT4216 - ubiquinone/menaquinone biosynthesis methyltransferase (NCBI ptt file), at 5,552,878 to 5,553,615 BT4216 Position (kb) 5551 5552Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 5550.232 kb on + strand, within BT4213at 5550.249 kb on + strand, within BT4213at 5550.250 kb on - strand, within BT4213at 5550.278 kb on + strand, within BT4213at 5550.279 kb on - strand, within BT4213at 5550.529 kb on - strand, within BT4213at 5550.574 kb on + strand, within BT4213at 5550.578 kb on + strand, within BT4213at 5550.821 kb on + strand, within BT4213at 5550.822 kb on - strand, within BT4213at 5550.856 kb on - strand, within BT4213at 5550.873 kb on + strand, within BT4213at 5550.877 kb on + strand, within BT4213at 5550.901 kb on - strand, within BT4213at 5550.923 kb on + strandat 5550.923 kb on + strandat 5550.925 kb on + strandat 5550.926 kb on - strandat 5550.972 kb on + strandat 5550.985 kb on - strandat 5551.058 kb on + strandat 5551.060 kb on + strandat 5551.061 kb on - strandat 5551.064 kb on - strandat 5551.080 kb on - strandat 5551.111 kb on + strandat 5551.112 kb on - strandat 5551.121 kb on + strandat 5551.225 kb on + strand, within BT4214at 5551.225 kb on + strand, within BT4214at 5551.225 kb on + strand, within BT4214at 5551.226 kb on - strand, within BT4214at 5551.226 kb on - strand, within BT4214at 5551.226 kb on - strand, within BT4214at 5551.230 kb on - strand, within BT4214at 5551.230 kb on - strand, within BT4214at 5551.340 kb on + strand, within BT4214at 5551.407 kb on + strand, within BT4214at 5551.448 kb on - strand, within BT4214at 5551.503 kb on - strand, within BT4214at 5551.503 kb on - strand, within BT4214at 5551.509 kb on + strand, within BT4214at 5551.513 kb on - strandat 5551.523 kb on + strand, within BT4214at 5551.649 kb on + strand, within BT4214at 5551.649 kb on + strand, within BT4214at 5551.649 kb on + strand, within BT4214at 5551.692 kb on - strand, within BT4214at 5551.697 kb on + strand, within BT4214at 5551.712 kb on - strand, within BT4214at 5551.773 kb on + strand, within BT4214at 5551.780 kb on - strand, within BT4214at 5551.780 kb on - strand, within BT4214at 5551.796 kb on + strand, within BT4214at 5551.797 kb on - strand, within BT4214at 5551.861 kb on + strandat 5551.875 kb on - strandat 5551.877 kb on - strandat 5551.877 kb on - strandat 5551.877 kb on - strandat 5551.877 kb on - strandat 5551.877 kb on - strandat 5551.905 kb on + strandat 5551.975 kb on - strandat 5551.988 kb on + strandat 5551.989 kb on - strandat 5551.991 kb on + strandat 5551.991 kb on + strandat 5551.992 kb on - strandat 5551.992 kb on - strandat 5552.021 kb on + strandat 5552.021 kb on + strandat 5552.022 kb on - strandat 5552.036 kb on - strandat 5552.057 kb on + strandat 5552.089 kb on + strandat 5552.090 kb on - strandat 5552.863 kb on - strandat 5552.863 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM
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5,550,232 + BT4213 0.15 -1.0
5,550,249 + BT4213 0.17 -2.6
5,550,250 - BT4213 0.17 +1.4
5,550,278 + BT4213 0.20 +1.0
5,550,279 - BT4213 0.21 -2.3
5,550,529 - BT4213 0.48 -1.3
5,550,574 + BT4213 0.53 -2.5
5,550,578 + BT4213 0.54 -4.8
5,550,821 + BT4213 0.81 -1.7
5,550,822 - BT4213 0.81 -0.0
5,550,856 - BT4213 0.84 -0.2
5,550,873 + BT4213 0.86 -0.7
5,550,877 + BT4213 0.87 -0.0
5,550,901 - BT4213 0.89 +1.7
5,550,923 + -1.0
5,550,923 + -0.0
5,550,925 + -2.0
5,550,926 - -1.0
5,550,972 + -2.8
5,550,985 - -0.0
5,551,058 + -4.6
5,551,060 + -1.0
5,551,061 - -1.0
5,551,064 - -1.3
5,551,080 - -2.3
5,551,111 + -0.0
5,551,112 - -3.0
5,551,121 + -2.0
5,551,225 + BT4214 0.18 -2.3
5,551,225 + BT4214 0.18 -0.3
5,551,225 + BT4214 0.18 -4.1
5,551,226 - BT4214 0.18 -2.6
5,551,226 - BT4214 0.18 -2.8
5,551,226 - BT4214 0.18 -3.0
5,551,230 - BT4214 0.19 -2.3
5,551,230 - BT4214 0.19 -3.3
5,551,340 + BT4214 0.31 -2.7
5,551,407 + BT4214 0.39 -1.0
5,551,448 - BT4214 0.44 -0.0
5,551,503 - BT4214 0.50 -2.3
5,551,503 - BT4214 0.50 -1.6
5,551,509 + BT4214 0.51 -1.7
5,551,513 - +0.6
5,551,523 + BT4214 0.52 -1.2
5,551,649 + BT4214 0.67 -3.3
5,551,649 + BT4214 0.67 -3.0
5,551,649 + BT4214 0.67 -2.0
5,551,692 - BT4214 0.71 -0.0
5,551,697 + BT4214 0.72 -2.9
5,551,712 - BT4214 0.74 -0.0
5,551,773 + BT4214 0.81 -1.0
5,551,780 - BT4214 0.82 -3.3
5,551,780 - BT4214 0.82 -1.0
5,551,796 + BT4214 0.83 -0.0
5,551,797 - BT4214 0.84 -3.5
5,551,861 + -2.8
5,551,875 - -2.0
5,551,877 - -1.6
5,551,877 - -3.3
5,551,877 - -4.6
5,551,877 - -0.8
5,551,877 - -0.0
5,551,905 + -1.6
5,551,975 - -4.0
5,551,988 + -1.0
5,551,989 - -2.8
5,551,991 + -2.6
5,551,991 + -2.8
5,551,992 - -3.0
5,551,992 - -0.0
5,552,021 + -1.0
5,552,021 + -0.9
5,552,022 - -1.0
5,552,036 - -1.6
5,552,057 + -2.0
5,552,089 + -2.8
5,552,090 - -1.6
5,552,863 - -2.3
5,552,863 - -2.3

Or see this region's nucleotide sequence