Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4213 and BT4214 are separated by 72 nucleotides BT4214 and BT4215 are separated by 152 nucleotides BT4215 and BT4216 are separated by 38 nucleotides
BT4213: BT4213 - conserved hypothetical protein (NCBI ptt file), at 5,550,093 to 5,550,995
BT4213
BT4214: BT4214 - conserved hypothetical protein (NCBI ptt file), at 5,551,068 to 5,551,940
BT4214
BT4215: BT4215 - shikimate dehydrogenase (EC 1.1.1.25) (from data) , at 5,552,093 to 5,552,839
BT4215
BT4216: BT4216 - ubiquinone/menaquinone biosynthesis methyltransferase (NCBI ptt file), at 5,552,878 to 5,553,615
BT4216
Position (kb)
5551
5552 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5550.232 kb on + strand, within BT4213 at 5550.249 kb on + strand, within BT4213 at 5550.250 kb on - strand, within BT4213 at 5550.278 kb on + strand, within BT4213 at 5550.279 kb on - strand, within BT4213 at 5550.529 kb on - strand, within BT4213 at 5550.574 kb on + strand, within BT4213 at 5550.578 kb on + strand, within BT4213 at 5550.821 kb on + strand, within BT4213 at 5550.822 kb on - strand, within BT4213 at 5550.856 kb on - strand, within BT4213 at 5550.873 kb on + strand, within BT4213 at 5550.877 kb on + strand, within BT4213 at 5550.901 kb on - strand, within BT4213 at 5550.923 kb on + strand at 5550.923 kb on + strand at 5550.925 kb on + strand at 5550.926 kb on - strand at 5550.972 kb on + strand at 5550.985 kb on - strand at 5551.058 kb on + strand at 5551.060 kb on + strand at 5551.061 kb on - strand at 5551.064 kb on - strand at 5551.080 kb on - strand at 5551.111 kb on + strand at 5551.112 kb on - strand at 5551.121 kb on + strand at 5551.225 kb on + strand, within BT4214 at 5551.225 kb on + strand, within BT4214 at 5551.225 kb on + strand, within BT4214 at 5551.226 kb on - strand, within BT4214 at 5551.226 kb on - strand, within BT4214 at 5551.226 kb on - strand, within BT4214 at 5551.230 kb on - strand, within BT4214 at 5551.230 kb on - strand, within BT4214 at 5551.340 kb on + strand, within BT4214 at 5551.407 kb on + strand, within BT4214 at 5551.448 kb on - strand, within BT4214 at 5551.503 kb on - strand, within BT4214 at 5551.503 kb on - strand, within BT4214 at 5551.509 kb on + strand, within BT4214 at 5551.513 kb on - strand at 5551.523 kb on + strand, within BT4214 at 5551.649 kb on + strand, within BT4214 at 5551.649 kb on + strand, within BT4214 at 5551.649 kb on + strand, within BT4214 at 5551.692 kb on - strand, within BT4214 at 5551.697 kb on + strand, within BT4214 at 5551.712 kb on - strand, within BT4214 at 5551.773 kb on + strand, within BT4214 at 5551.780 kb on - strand, within BT4214 at 5551.780 kb on - strand, within BT4214 at 5551.796 kb on + strand, within BT4214 at 5551.797 kb on - strand, within BT4214 at 5551.861 kb on + strand at 5551.875 kb on - strand at 5551.877 kb on - strand at 5551.877 kb on - strand at 5551.877 kb on - strand at 5551.877 kb on - strand at 5551.877 kb on - strand at 5551.905 kb on + strand at 5551.975 kb on - strand at 5551.988 kb on + strand at 5551.989 kb on - strand at 5551.991 kb on + strand at 5551.991 kb on + strand at 5551.992 kb on - strand at 5551.992 kb on - strand at 5552.021 kb on + strand at 5552.021 kb on + strand at 5552.022 kb on - strand at 5552.036 kb on - strand at 5552.057 kb on + strand at 5552.089 kb on + strand at 5552.090 kb on - strand at 5552.863 kb on - strand at 5552.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 5,550,232 + BT4213 0.15 -1.0 5,550,249 + BT4213 0.17 -2.6 5,550,250 - BT4213 0.17 +1.4 5,550,278 + BT4213 0.20 +1.0 5,550,279 - BT4213 0.21 -2.3 5,550,529 - BT4213 0.48 -1.3 5,550,574 + BT4213 0.53 -2.5 5,550,578 + BT4213 0.54 -4.8 5,550,821 + BT4213 0.81 -1.7 5,550,822 - BT4213 0.81 -0.0 5,550,856 - BT4213 0.84 -0.2 5,550,873 + BT4213 0.86 -0.7 5,550,877 + BT4213 0.87 -0.0 5,550,901 - BT4213 0.89 +1.7 5,550,923 + -1.0 5,550,923 + -0.0 5,550,925 + -2.0 5,550,926 - -1.0 5,550,972 + -2.8 5,550,985 - -0.0 5,551,058 + -4.6 5,551,060 + -1.0 5,551,061 - -1.0 5,551,064 - -1.3 5,551,080 - -2.3 5,551,111 + -0.0 5,551,112 - -3.0 5,551,121 + -2.0 5,551,225 + BT4214 0.18 -2.3 5,551,225 + BT4214 0.18 -0.3 5,551,225 + BT4214 0.18 -4.1 5,551,226 - BT4214 0.18 -2.6 5,551,226 - BT4214 0.18 -2.8 5,551,226 - BT4214 0.18 -3.0 5,551,230 - BT4214 0.19 -2.3 5,551,230 - BT4214 0.19 -3.3 5,551,340 + BT4214 0.31 -2.7 5,551,407 + BT4214 0.39 -1.0 5,551,448 - BT4214 0.44 -0.0 5,551,503 - BT4214 0.50 -2.3 5,551,503 - BT4214 0.50 -1.6 5,551,509 + BT4214 0.51 -1.7 5,551,513 - +0.6 5,551,523 + BT4214 0.52 -1.2 5,551,649 + BT4214 0.67 -3.3 5,551,649 + BT4214 0.67 -3.0 5,551,649 + BT4214 0.67 -2.0 5,551,692 - BT4214 0.71 -0.0 5,551,697 + BT4214 0.72 -2.9 5,551,712 - BT4214 0.74 -0.0 5,551,773 + BT4214 0.81 -1.0 5,551,780 - BT4214 0.82 -3.3 5,551,780 - BT4214 0.82 -1.0 5,551,796 + BT4214 0.83 -0.0 5,551,797 - BT4214 0.84 -3.5 5,551,861 + -2.8 5,551,875 - -2.0 5,551,877 - -1.6 5,551,877 - -3.3 5,551,877 - -4.6 5,551,877 - -0.8 5,551,877 - -0.0 5,551,905 + -1.6 5,551,975 - -4.0 5,551,988 + -1.0 5,551,989 - -2.8 5,551,991 + -2.6 5,551,991 + -2.8 5,551,992 - -3.0 5,551,992 - -0.0 5,552,021 + -1.0 5,552,021 + -0.9 5,552,022 - -1.0 5,552,036 - -1.6 5,552,057 + -2.0 5,552,089 + -2.8 5,552,090 - -1.6 5,552,863 - -2.3 5,552,863 - -2.3
Or see this region's nucleotide sequence