Experiment: BHIS with Simvastatin 30 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1116 and BT1117 are separated by 97 nucleotides BT1117 and BT1118 are separated by 9 nucleotides BT1118 and BT1119 are separated by 36 nucleotides
BT1116: BT1116 - conserved hypothetical protein (NCBI ptt file), at 1,402,938 to 1,403,495
BT1116
BT1117: BT1117 - conserved hypothetical protein, with a cAMP-binding domain (NCBI ptt file), at 1,403,593 to 1,403,958
BT1117
BT1118: BT1118 - outer membrane binding protein for D-galacturonate and 1,4-beta-D-galactobiose (SusD-like) (from data) , at 1,403,968 to 1,405,560
BT1118
BT1119: BT1119 - TonB-dependent receptor for D-galacturonate and 1,4-beta-D-galactobiose (SusC-like) (from data) , at 1,405,597 to 1,408,293
BT1119
Position (kb)
1403
1404
1405
1406 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1402.968 kb on + strand at 1402.981 kb on + strand at 1402.992 kb on + strand at 1403.003 kb on + strand, within BT1116 at 1403.110 kb on + strand, within BT1116 at 1403.110 kb on + strand, within BT1116 at 1403.133 kb on + strand, within BT1116 at 1403.133 kb on + strand at 1403.133 kb on + strand at 1403.135 kb on + strand, within BT1116 at 1403.155 kb on - strand, within BT1116 at 1403.180 kb on + strand, within BT1116 at 1403.188 kb on + strand, within BT1116 at 1403.321 kb on + strand, within BT1116 at 1403.333 kb on + strand, within BT1116 at 1403.362 kb on + strand, within BT1116 at 1403.490 kb on + strand at 1403.496 kb on - strand at 1403.565 kb on + strand at 1403.616 kb on + strand at 1403.616 kb on + strand at 1403.664 kb on + strand, within BT1117 at 1403.738 kb on - strand, within BT1117 at 1403.758 kb on + strand, within BT1117 at 1403.762 kb on + strand, within BT1117 at 1403.953 kb on - strand at 1404.010 kb on + strand at 1404.042 kb on + strand at 1404.042 kb on + strand at 1404.114 kb on + strand at 1404.180 kb on + strand, within BT1118 at 1404.181 kb on - strand, within BT1118 at 1404.198 kb on + strand, within BT1118 at 1404.199 kb on - strand, within BT1118 at 1404.254 kb on - strand, within BT1118 at 1404.520 kb on + strand, within BT1118 at 1404.547 kb on + strand, within BT1118 at 1404.561 kb on + strand, within BT1118 at 1404.722 kb on + strand, within BT1118 at 1404.875 kb on + strand, within BT1118 at 1404.916 kb on - strand, within BT1118 at 1404.919 kb on + strand, within BT1118 at 1404.919 kb on + strand, within BT1118 at 1404.941 kb on + strand, within BT1118 at 1404.941 kb on + strand, within BT1118 at 1404.944 kb on + strand, within BT1118 at 1405.095 kb on - strand, within BT1118 at 1405.151 kb on + strand, within BT1118 at 1405.187 kb on + strand, within BT1118 at 1405.200 kb on + strand, within BT1118 at 1405.284 kb on + strand, within BT1118 at 1405.296 kb on + strand, within BT1118 at 1405.296 kb on + strand, within BT1118 at 1405.362 kb on + strand, within BT1118 at 1405.379 kb on + strand, within BT1118 at 1405.461 kb on + strand at 1405.462 kb on - strand at 1405.565 kb on - strand at 1405.570 kb on + strand at 1405.570 kb on + strand at 1405.570 kb on + strand at 1405.570 kb on + strand at 1405.643 kb on + strand at 1405.681 kb on + strand at 1405.682 kb on + strand at 1405.682 kb on - strand at 1405.715 kb on + strand at 1405.802 kb on - strand at 1405.897 kb on + strand, within BT1119 at 1405.904 kb on + strand, within BT1119 at 1405.929 kb on + strand, within BT1119 at 1405.959 kb on + strand, within BT1119 at 1405.959 kb on + strand, within BT1119 at 1406.035 kb on + strand, within BT1119 at 1406.036 kb on - strand, within BT1119 at 1406.064 kb on - strand, within BT1119 at 1406.123 kb on + strand, within BT1119 at 1406.129 kb on + strand, within BT1119 at 1406.209 kb on + strand, within BT1119 at 1406.212 kb on + strand, within BT1119 at 1406.297 kb on + strand, within BT1119 at 1406.340 kb on + strand, within BT1119 at 1406.341 kb on - strand, within BT1119 at 1406.483 kb on + strand, within BT1119 at 1406.510 kb on + strand, within BT1119 at 1406.545 kb on + strand, within BT1119 at 1406.547 kb on + strand, within BT1119
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Simvastatin 30 uM remove 1,402,968 + +0.9 1,402,981 + -1.2 1,402,992 + -1.8 1,403,003 + BT1116 0.12 -1.4 1,403,110 + BT1116 0.31 -1.2 1,403,110 + BT1116 0.31 -0.2 1,403,133 + BT1116 0.35 -1.2 1,403,133 + -0.2 1,403,133 + -0.2 1,403,135 + BT1116 0.35 -2.2 1,403,155 - BT1116 0.39 -2.2 1,403,180 + BT1116 0.43 -2.2 1,403,188 + BT1116 0.45 -2.0 1,403,321 + BT1116 0.69 +0.8 1,403,333 + BT1116 0.71 -1.5 1,403,362 + BT1116 0.76 -3.0 1,403,490 + -1.8 1,403,496 - -0.7 1,403,565 + -1.0 1,403,616 + -1.6 1,403,616 + -2.5 1,403,664 + BT1117 0.19 -3.2 1,403,738 - BT1117 0.40 -1.8 1,403,758 + BT1117 0.45 -2.2 1,403,762 + BT1117 0.46 -2.8 1,403,953 - -0.2 1,404,010 + -2.5 1,404,042 + +0.6 1,404,042 + -1.4 1,404,114 + -0.2 1,404,180 + BT1118 0.13 -0.2 1,404,181 - BT1118 0.13 -0.2 1,404,198 + BT1118 0.14 -3.2 1,404,199 - BT1118 0.15 -1.2 1,404,254 - BT1118 0.18 -2.2 1,404,520 + BT1118 0.35 -3.4 1,404,547 + BT1118 0.36 -3.2 1,404,561 + BT1118 0.37 -1.2 1,404,722 + BT1118 0.47 -2.8 1,404,875 + BT1118 0.57 -2.0 1,404,916 - BT1118 0.60 -1.8 1,404,919 + BT1118 0.60 -2.2 1,404,919 + BT1118 0.60 -1.2 1,404,941 + BT1118 0.61 -3.8 1,404,941 + BT1118 0.61 -3.5 1,404,944 + BT1118 0.61 -1.4 1,405,095 - BT1118 0.71 -1.8 1,405,151 + BT1118 0.74 -0.2 1,405,187 + BT1118 0.77 -1.5 1,405,200 + BT1118 0.77 -3.6 1,405,284 + BT1118 0.83 -1.8 1,405,296 + BT1118 0.83 -1.2 1,405,296 + BT1118 0.83 -1.8 1,405,362 + BT1118 0.88 -1.2 1,405,379 + BT1118 0.89 -2.8 1,405,461 + -0.0 1,405,462 - -1.8 1,405,565 - -1.8 1,405,570 + -0.8 1,405,570 + -0.2 1,405,570 + -0.2 1,405,570 + -2.8 1,405,643 + -1.0 1,405,681 + -2.5 1,405,682 + -3.5 1,405,682 - -0.2 1,405,715 + -3.3 1,405,802 - -0.2 1,405,897 + BT1119 0.11 -3.0 1,405,904 + BT1119 0.11 -2.5 1,405,929 + BT1119 0.12 -1.4 1,405,959 + BT1119 0.13 -1.2 1,405,959 + BT1119 0.13 -1.2 1,406,035 + BT1119 0.16 -3.0 1,406,036 - BT1119 0.16 -2.8 1,406,064 - BT1119 0.17 -1.2 1,406,123 + BT1119 0.20 -1.2 1,406,129 + BT1119 0.20 -3.8 1,406,209 + BT1119 0.23 -1.8 1,406,212 + BT1119 0.23 -2.7 1,406,297 + BT1119 0.26 -2.8 1,406,340 + BT1119 0.28 -4.2 1,406,341 - BT1119 0.28 -1.8 1,406,483 + BT1119 0.33 -1.8 1,406,510 + BT1119 0.34 -1.2 1,406,545 + BT1119 0.35 -0.5 1,406,547 + BT1119 0.35 -1.0
Or see this region's nucleotide sequence