Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_278

Experiment: Cobalt chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_277 and Pf6N2E2_278 are separated by 871 nucleotidesPf6N2E2_278 and Pf6N2E2_279 are separated by 107 nucleotides Pf6N2E2_277 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 342,559 to 342,972 _277 Pf6N2E2_278 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 343,844 to 345,766 _278 Pf6N2E2_279 - Isocitrate lyase (EC 4.1.3.1), at 345,874 to 347,199 _279 Position (kb) 343 344 345 346Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 343.016 kb on - strandat 343.036 kb on - strandat 343.106 kb on - strandat 343.118 kb on - strandat 343.125 kb on + strandat 343.126 kb on - strandat 343.189 kb on - strandat 343.189 kb on - strandat 343.269 kb on + strandat 343.269 kb on + strandat 343.269 kb on + strandat 343.269 kb on + strandat 343.270 kb on - strandat 343.270 kb on - strandat 343.294 kb on + strandat 343.294 kb on + strandat 343.294 kb on + strandat 343.294 kb on + strandat 343.332 kb on + strandat 343.354 kb on + strandat 343.549 kb on + strandat 343.550 kb on - strandat 343.556 kb on + strandat 343.556 kb on + strandat 343.556 kb on + strandat 343.556 kb on + strandat 343.556 kb on + strandat 343.557 kb on - strandat 343.557 kb on - strandat 343.634 kb on + strandat 343.634 kb on + strandat 343.635 kb on - strandat 343.701 kb on - strandat 343.701 kb on - strandat 343.752 kb on - strandat 343.752 kb on - strandat 343.759 kb on + strandat 343.759 kb on + strandat 343.775 kb on - strandat 343.792 kb on + strandat 343.792 kb on + strandat 343.792 kb on + strandat 343.792 kb on + strandat 343.792 kb on + strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.793 kb on - strandat 343.796 kb on + strandat 343.796 kb on + strandat 343.797 kb on - strandat 343.797 kb on - strandat 343.797 kb on - strandat 343.797 kb on - strandat 343.797 kb on - strandat 343.829 kb on + strandat 343.830 kb on - strandat 343.991 kb on - strandat 344.039 kb on + strand, within Pf6N2E2_278at 344.206 kb on + strand, within Pf6N2E2_278at 344.206 kb on + strand, within Pf6N2E2_278at 344.307 kb on + strand, within Pf6N2E2_278at 344.308 kb on - strand, within Pf6N2E2_278at 344.308 kb on - strand, within Pf6N2E2_278at 344.349 kb on - strand, within Pf6N2E2_278at 344.393 kb on + strand, within Pf6N2E2_278at 344.510 kb on + strand, within Pf6N2E2_278at 344.511 kb on - strand, within Pf6N2E2_278at 344.585 kb on - strand, within Pf6N2E2_278at 344.585 kb on - strand, within Pf6N2E2_278at 344.660 kb on + strand, within Pf6N2E2_278at 344.848 kb on + strand, within Pf6N2E2_278at 344.848 kb on + strand, within Pf6N2E2_278at 344.893 kb on + strand, within Pf6N2E2_278at 344.894 kb on - strand, within Pf6N2E2_278at 345.469 kb on + strand, within Pf6N2E2_278at 345.661 kb on - strandat 345.661 kb on - strandat 346.001 kb on + strandat 346.002 kb on - strandat 346.071 kb on - strand, within Pf6N2E2_279at 346.142 kb on + strand, within Pf6N2E2_279at 346.143 kb on - strand, within Pf6N2E2_279at 346.229 kb on + strand, within Pf6N2E2_279at 346.229 kb on + strand, within Pf6N2E2_279at 346.229 kb on + strand, within Pf6N2E2_279at 346.230 kb on - strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.389 kb on - strand, within Pf6N2E2_279at 346.389 kb on - strand, within Pf6N2E2_279at 346.389 kb on - strand, within Pf6N2E2_279at 346.480 kb on - strand, within Pf6N2E2_279at 346.670 kb on + strand, within Pf6N2E2_279at 346.728 kb on - strand, within Pf6N2E2_279at 346.736 kb on + strand, within Pf6N2E2_279at 346.737 kb on - strand, within Pf6N2E2_279at 346.737 kb on - strand, within Pf6N2E2_279at 346.737 kb on - strand, within Pf6N2E2_279

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM
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343,016 - +3.8
343,036 - +3.0
343,106 - +1.6
343,118 - +0.8
343,125 + +4.0
343,126 - -0.2
343,189 - -1.8
343,189 - -1.2
343,269 + +3.9
343,269 + +4.5
343,269 + +4.7
343,269 + +4.5
343,270 - -0.6
343,270 - +0.8
343,294 + +3.9
343,294 + +4.6
343,294 + +3.8
343,294 + +4.0
343,332 + +4.1
343,354 + +5.0
343,549 + +3.4
343,550 - -0.2
343,556 + +3.3
343,556 + +2.0
343,556 + +3.8
343,556 + +4.6
343,556 + +3.1
343,557 - +0.6
343,557 - -0.2
343,634 + +2.7
343,634 + +2.8
343,635 - -1.3
343,701 - -0.2
343,701 - -0.5
343,752 - +1.0
343,752 - -0.4
343,759 + +2.6
343,759 + +2.2
343,775 - +1.1
343,792 + +2.1
343,792 + +2.7
343,792 + +2.8
343,792 + +0.2
343,792 + +2.0
343,793 - +0.6
343,793 - -0.4
343,793 - -0.8
343,793 - +1.4
343,793 - -0.1
343,793 - -0.2
343,793 - -0.4
343,793 - -0.2
343,793 - +0.8
343,793 - +1.4
343,793 - +1.4
343,796 + +2.2
343,796 + +1.9
343,797 - -1.5
343,797 - -0.4
343,797 - -0.2
343,797 - +0.3
343,797 - -0.7
343,829 + +2.1
343,830 - -1.0
343,991 - -0.4
344,039 + Pf6N2E2_278 0.10 +0.8
344,206 + Pf6N2E2_278 0.19 +0.6
344,206 + Pf6N2E2_278 0.19 +1.4
344,307 + Pf6N2E2_278 0.24 +2.7
344,308 - Pf6N2E2_278 0.24 +0.0
344,308 - Pf6N2E2_278 0.24 +0.8
344,349 - Pf6N2E2_278 0.26 -0.2
344,393 + Pf6N2E2_278 0.29 -0.2
344,510 + Pf6N2E2_278 0.35 +3.1
344,511 - Pf6N2E2_278 0.35 +1.1
344,585 - Pf6N2E2_278 0.39 -0.1
344,585 - Pf6N2E2_278 0.39 -0.6
344,660 + Pf6N2E2_278 0.42 +1.0
344,848 + Pf6N2E2_278 0.52 +0.8
344,848 + Pf6N2E2_278 0.52 +1.0
344,893 + Pf6N2E2_278 0.55 +1.3
344,894 - Pf6N2E2_278 0.55 +0.7
345,469 + Pf6N2E2_278 0.85 -0.0
345,661 - +0.0
345,661 - +0.8
346,001 + +0.1
346,002 - +0.3
346,071 - Pf6N2E2_279 0.15 +1.3
346,142 + Pf6N2E2_279 0.20 +0.4
346,143 - Pf6N2E2_279 0.20 -0.3
346,229 + Pf6N2E2_279 0.27 +0.8
346,229 + Pf6N2E2_279 0.27 +1.6
346,229 + Pf6N2E2_279 0.27 -0.8
346,230 - Pf6N2E2_279 0.27 +1.8
346,388 + Pf6N2E2_279 0.39 -0.2
346,388 + Pf6N2E2_279 0.39 +0.8
346,388 + Pf6N2E2_279 0.39 -0.0
346,388 + Pf6N2E2_279 0.39 -1.4
346,388 + Pf6N2E2_279 0.39 -1.2
346,388 + Pf6N2E2_279 0.39 +1.0
346,389 - Pf6N2E2_279 0.39 -0.8
346,389 - Pf6N2E2_279 0.39 -0.5
346,389 - Pf6N2E2_279 0.39 +0.6
346,480 - Pf6N2E2_279 0.46 +0.6
346,670 + Pf6N2E2_279 0.60 -0.2
346,728 - Pf6N2E2_279 0.64 -1.5
346,736 + Pf6N2E2_279 0.65 -0.8
346,737 - Pf6N2E2_279 0.65 -0.8
346,737 - Pf6N2E2_279 0.65 +0.1
346,737 - Pf6N2E2_279 0.65 +0.1

Or see this region's nucleotide sequence