Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4406 and Pf6N2E2_4407 overlap by 4 nucleotides Pf6N2E2_4407 and Pf6N2E2_4408 are separated by 3 nucleotides
Pf6N2E2_4406 - 2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, flavoprotein, at 5,139,480 to 5,141,114
_4406
Pf6N2E2_4407 - 2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, gamma subunit, at 5,141,111 to 5,141,659
_4407
Pf6N2E2_4408 - 2-Keto-D-gluconate dehydrogenase (EC 1.1.99.4), membrane-bound, cytochrome c, at 5,141,663 to 5,143,087
_4408
Position (kb)
5141
5142 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5140.170 kb on - strand, within Pf6N2E2_4406 at 5140.170 kb on - strand, within Pf6N2E2_4406 at 5140.238 kb on - strand, within Pf6N2E2_4406 at 5140.238 kb on - strand, within Pf6N2E2_4406 at 5140.238 kb on - strand, within Pf6N2E2_4406 at 5140.255 kb on + strand, within Pf6N2E2_4406 at 5140.255 kb on - strand, within Pf6N2E2_4406 at 5140.297 kb on + strand, within Pf6N2E2_4406 at 5140.297 kb on + strand, within Pf6N2E2_4406 at 5140.298 kb on - strand, within Pf6N2E2_4406 at 5140.298 kb on - strand, within Pf6N2E2_4406 at 5140.394 kb on + strand, within Pf6N2E2_4406 at 5140.394 kb on + strand, within Pf6N2E2_4406 at 5140.395 kb on - strand, within Pf6N2E2_4406 at 5140.411 kb on + strand at 5140.411 kb on + strand, within Pf6N2E2_4406 at 5140.412 kb on - strand, within Pf6N2E2_4406 at 5140.412 kb on - strand, within Pf6N2E2_4406 at 5140.519 kb on + strand, within Pf6N2E2_4406 at 5140.561 kb on + strand, within Pf6N2E2_4406 at 5140.561 kb on + strand, within Pf6N2E2_4406 at 5140.561 kb on + strand, within Pf6N2E2_4406 at 5140.561 kb on + strand, within Pf6N2E2_4406 at 5140.561 kb on + strand, within Pf6N2E2_4406 at 5140.561 kb on + strand, within Pf6N2E2_4406 at 5140.562 kb on - strand, within Pf6N2E2_4406 at 5140.562 kb on - strand, within Pf6N2E2_4406 at 5140.562 kb on - strand, within Pf6N2E2_4406 at 5140.562 kb on - strand, within Pf6N2E2_4406 at 5140.562 kb on - strand, within Pf6N2E2_4406 at 5140.562 kb on - strand, within Pf6N2E2_4406 at 5140.610 kb on - strand, within Pf6N2E2_4406 at 5140.685 kb on - strand, within Pf6N2E2_4406 at 5140.685 kb on - strand, within Pf6N2E2_4406 at 5140.685 kb on - strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.705 kb on + strand, within Pf6N2E2_4406 at 5140.706 kb on - strand, within Pf6N2E2_4406 at 5140.706 kb on - strand, within Pf6N2E2_4406 at 5140.823 kb on - strand, within Pf6N2E2_4406 at 5140.834 kb on + strand, within Pf6N2E2_4406 at 5140.834 kb on + strand, within Pf6N2E2_4406 at 5140.835 kb on - strand at 5140.861 kb on + strand, within Pf6N2E2_4406 at 5140.870 kb on + strand, within Pf6N2E2_4406 at 5140.870 kb on + strand, within Pf6N2E2_4406 at 5140.870 kb on + strand, within Pf6N2E2_4406 at 5140.870 kb on + strand, within Pf6N2E2_4406 at 5140.871 kb on - strand, within Pf6N2E2_4406 at 5140.871 kb on - strand, within Pf6N2E2_4406 at 5140.871 kb on - strand, within Pf6N2E2_4406 at 5140.871 kb on - strand, within Pf6N2E2_4406 at 5141.098 kb on + strand at 5141.098 kb on + strand at 5141.098 kb on + strand at 5141.098 kb on + strand at 5141.099 kb on - strand at 5141.099 kb on - strand at 5141.099 kb on - strand at 5141.114 kb on + strand at 5141.114 kb on + strand at 5141.115 kb on - strand at 5141.115 kb on - strand at 5141.115 kb on - strand at 5141.115 kb on - strand at 5141.172 kb on + strand, within Pf6N2E2_4407 at 5141.172 kb on + strand, within Pf6N2E2_4407 at 5141.172 kb on + strand, within Pf6N2E2_4407 at 5141.208 kb on + strand, within Pf6N2E2_4407 at 5141.208 kb on + strand, within Pf6N2E2_4407 at 5141.209 kb on - strand, within Pf6N2E2_4407 at 5141.209 kb on - strand, within Pf6N2E2_4407 at 5141.272 kb on - strand, within Pf6N2E2_4407 at 5141.272 kb on - strand, within Pf6N2E2_4407 at 5141.337 kb on + strand, within Pf6N2E2_4407 at 5141.337 kb on + strand, within Pf6N2E2_4407 at 5141.337 kb on + strand, within Pf6N2E2_4407 at 5141.338 kb on - strand, within Pf6N2E2_4407 at 5141.338 kb on - strand, within Pf6N2E2_4407 at 5141.433 kb on + strand, within Pf6N2E2_4407 at 5141.437 kb on - strand, within Pf6N2E2_4407 at 5141.612 kb on - strand at 5141.700 kb on + strand at 5141.700 kb on + strand at 5141.701 kb on - strand at 5141.701 kb on - strand at 5141.751 kb on + strand at 5141.751 kb on + strand at 5141.795 kb on + strand at 5141.796 kb on - strand at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.856 kb on + strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.857 kb on - strand, within Pf6N2E2_4408 at 5141.967 kb on + strand, within Pf6N2E2_4408 at 5141.968 kb on - strand, within Pf6N2E2_4408 at 5142.247 kb on - strand, within Pf6N2E2_4408 at 5142.247 kb on - strand, within Pf6N2E2_4408 at 5142.247 kb on - strand, within Pf6N2E2_4408 at 5142.394 kb on - strand, within Pf6N2E2_4408 at 5142.501 kb on + strand, within Pf6N2E2_4408 at 5142.502 kb on - strand, within Pf6N2E2_4408 at 5142.502 kb on - strand, within Pf6N2E2_4408 at 5142.502 kb on - strand, within Pf6N2E2_4408
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 5,140,170 - Pf6N2E2_4406 0.42 +0.7 5,140,170 - Pf6N2E2_4406 0.42 -0.7 5,140,238 - Pf6N2E2_4406 0.46 +0.4 5,140,238 - Pf6N2E2_4406 0.46 -1.2 5,140,238 - Pf6N2E2_4406 0.46 +0.8 5,140,255 + Pf6N2E2_4406 0.47 +0.2 5,140,255 - Pf6N2E2_4406 0.47 -0.8 5,140,297 + Pf6N2E2_4406 0.50 -0.8 5,140,297 + Pf6N2E2_4406 0.50 -0.7 5,140,298 - Pf6N2E2_4406 0.50 -0.3 5,140,298 - Pf6N2E2_4406 0.50 -1.4 5,140,394 + Pf6N2E2_4406 0.56 +0.9 5,140,394 + Pf6N2E2_4406 0.56 +0.7 5,140,395 - Pf6N2E2_4406 0.56 +0.1 5,140,411 + +0.4 5,140,411 + Pf6N2E2_4406 0.57 +0.3 5,140,412 - Pf6N2E2_4406 0.57 +0.5 5,140,412 - Pf6N2E2_4406 0.57 +0.3 5,140,519 + Pf6N2E2_4406 0.64 +0.2 5,140,561 + Pf6N2E2_4406 0.66 +0.7 5,140,561 + Pf6N2E2_4406 0.66 -0.5 5,140,561 + Pf6N2E2_4406 0.66 +0.4 5,140,561 + Pf6N2E2_4406 0.66 +0.4 5,140,561 + Pf6N2E2_4406 0.66 -0.3 5,140,561 + Pf6N2E2_4406 0.66 +0.3 5,140,562 - Pf6N2E2_4406 0.66 +1.2 5,140,562 - Pf6N2E2_4406 0.66 +0.7 5,140,562 - Pf6N2E2_4406 0.66 -0.2 5,140,562 - Pf6N2E2_4406 0.66 +0.5 5,140,562 - Pf6N2E2_4406 0.66 +0.5 5,140,562 - Pf6N2E2_4406 0.66 +0.6 5,140,610 - Pf6N2E2_4406 0.69 +1.4 5,140,685 - Pf6N2E2_4406 0.74 +0.3 5,140,685 - Pf6N2E2_4406 0.74 -1.0 5,140,685 - Pf6N2E2_4406 0.74 +0.4 5,140,705 + Pf6N2E2_4406 0.75 -0.7 5,140,705 + Pf6N2E2_4406 0.75 -0.1 5,140,705 + Pf6N2E2_4406 0.75 +1.3 5,140,705 + Pf6N2E2_4406 0.75 -0.8 5,140,705 + Pf6N2E2_4406 0.75 -0.2 5,140,706 - Pf6N2E2_4406 0.75 -0.4 5,140,706 - Pf6N2E2_4406 0.75 -0.3 5,140,823 - Pf6N2E2_4406 0.82 -0.2 5,140,834 + Pf6N2E2_4406 0.83 +0.4 5,140,834 + Pf6N2E2_4406 0.83 +1.3 5,140,835 - +0.4 5,140,861 + Pf6N2E2_4406 0.84 +0.4 5,140,870 + Pf6N2E2_4406 0.85 +0.2 5,140,870 + Pf6N2E2_4406 0.85 -1.8 5,140,870 + Pf6N2E2_4406 0.85 -1.7 5,140,870 + Pf6N2E2_4406 0.85 -1.4 5,140,871 - Pf6N2E2_4406 0.85 +0.2 5,140,871 - Pf6N2E2_4406 0.85 -1.0 5,140,871 - Pf6N2E2_4406 0.85 +0.5 5,140,871 - Pf6N2E2_4406 0.85 +0.6 5,141,098 + -0.3 5,141,098 + -1.3 5,141,098 + -0.9 5,141,098 + +0.0 5,141,099 - -1.3 5,141,099 - -1.2 5,141,099 - -0.1 5,141,114 + -0.9 5,141,114 + -0.3 5,141,115 - +0.0 5,141,115 - -1.6 5,141,115 - -0.9 5,141,115 - -0.7 5,141,172 + Pf6N2E2_4407 0.11 +1.7 5,141,172 + Pf6N2E2_4407 0.11 +0.3 5,141,172 + Pf6N2E2_4407 0.11 +0.8 5,141,208 + Pf6N2E2_4407 0.18 +0.9 5,141,208 + Pf6N2E2_4407 0.18 +0.0 5,141,209 - Pf6N2E2_4407 0.18 +0.6 5,141,209 - Pf6N2E2_4407 0.18 -0.2 5,141,272 - Pf6N2E2_4407 0.29 +0.0 5,141,272 - Pf6N2E2_4407 0.29 +0.9 5,141,337 + Pf6N2E2_4407 0.41 +1.1 5,141,337 + Pf6N2E2_4407 0.41 +0.8 5,141,337 + Pf6N2E2_4407 0.41 +0.4 5,141,338 - Pf6N2E2_4407 0.41 -0.4 5,141,338 - Pf6N2E2_4407 0.41 +0.8 5,141,433 + Pf6N2E2_4407 0.59 -1.0 5,141,437 - Pf6N2E2_4407 0.59 +0.7 5,141,612 - -0.9 5,141,700 + +0.4 5,141,700 + -0.8 5,141,701 - +0.8 5,141,701 - +0.4 5,141,751 + +0.9 5,141,751 + -0.6 5,141,795 + -1.8 5,141,796 - -0.6 5,141,856 + Pf6N2E2_4408 0.14 +1.0 5,141,856 + Pf6N2E2_4408 0.14 -2.4 5,141,856 + Pf6N2E2_4408 0.14 -0.4 5,141,856 + Pf6N2E2_4408 0.14 +0.1 5,141,856 + Pf6N2E2_4408 0.14 -0.7 5,141,857 - Pf6N2E2_4408 0.14 +0.6 5,141,857 - Pf6N2E2_4408 0.14 +0.8 5,141,857 - Pf6N2E2_4408 0.14 -1.0 5,141,857 - Pf6N2E2_4408 0.14 +1.0 5,141,857 - Pf6N2E2_4408 0.14 -0.4 5,141,857 - Pf6N2E2_4408 0.14 -0.1 5,141,857 - Pf6N2E2_4408 0.14 +1.2 5,141,857 - Pf6N2E2_4408 0.14 +0.7 5,141,857 - Pf6N2E2_4408 0.14 -0.4 5,141,857 - Pf6N2E2_4408 0.14 +0.7 5,141,857 - Pf6N2E2_4408 0.14 -1.2 5,141,857 - Pf6N2E2_4408 0.14 +0.5 5,141,857 - Pf6N2E2_4408 0.14 -0.8 5,141,857 - Pf6N2E2_4408 0.14 -0.5 5,141,967 + Pf6N2E2_4408 0.21 -0.3 5,141,968 - Pf6N2E2_4408 0.21 -1.1 5,142,247 - Pf6N2E2_4408 0.41 +0.5 5,142,247 - Pf6N2E2_4408 0.41 +0.1 5,142,247 - Pf6N2E2_4408 0.41 -0.1 5,142,394 - Pf6N2E2_4408 0.51 +0.9 5,142,501 + Pf6N2E2_4408 0.59 -2.8 5,142,502 - Pf6N2E2_4408 0.59 +0.9 5,142,502 - Pf6N2E2_4408 0.59 +1.2 5,142,502 - Pf6N2E2_4408 0.59 -0.6
Or see this region's nucleotide sequence