Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_611

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_610 and Pf6N2E2_611 are separated by 219 nucleotidesPf6N2E2_611 and Pf6N2E2_612 are separated by 12 nucleotides Pf6N2E2_610 - Nitrate/nitrite transporter, at 693,285 to 694,607 _610 Pf6N2E2_611 - 2-dehydro-3-deoxy-L-arabinonate dehydratase (EC 4.2.1.43) (from data), at 694,827 to 695,819 _611 Pf6N2E2_612 - Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (from data), at 695,832 to 697,412 _612 Position (kb) 694 695 696Strain fitness (log2 ratio) -2 -1 0 1at 693.865 kb on - strand, within Pf6N2E2_610at 694.027 kb on - strand, within Pf6N2E2_610at 694.134 kb on + strand, within Pf6N2E2_610at 694.134 kb on + strand, within Pf6N2E2_610at 694.134 kb on + strand, within Pf6N2E2_610at 694.135 kb on - strand, within Pf6N2E2_610at 694.218 kb on + strand, within Pf6N2E2_610at 694.218 kb on + strand, within Pf6N2E2_610at 694.218 kb on + strand, within Pf6N2E2_610at 694.219 kb on - strand, within Pf6N2E2_610at 694.219 kb on - strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.222 kb on + strand, within Pf6N2E2_610at 694.223 kb on - strand, within Pf6N2E2_610at 694.416 kb on + strand, within Pf6N2E2_610at 694.452 kb on + strand, within Pf6N2E2_610at 694.479 kb on + strandat 694.480 kb on - strandat 694.601 kb on + strandat 694.796 kb on + strandat 694.796 kb on + strandat 694.797 kb on - strandat 694.834 kb on + strandat 694.835 kb on - strandat 694.835 kb on - strandat 695.007 kb on + strand, within Pf6N2E2_611at 695.245 kb on - strand, within Pf6N2E2_611at 695.343 kb on + strand, within Pf6N2E2_611at 695.343 kb on + strandat 695.343 kb on + strand, within Pf6N2E2_611at 695.343 kb on + strand, within Pf6N2E2_611at 695.344 kb on - strand, within Pf6N2E2_611at 695.344 kb on - strand, within Pf6N2E2_611at 695.430 kb on + strand, within Pf6N2E2_611at 695.430 kb on + strand, within Pf6N2E2_611at 695.431 kb on - strand, within Pf6N2E2_611at 695.431 kb on - strand, within Pf6N2E2_611at 695.431 kb on - strand, within Pf6N2E2_611at 695.436 kb on + strand, within Pf6N2E2_611at 695.625 kb on + strand, within Pf6N2E2_611at 695.626 kb on - strand, within Pf6N2E2_611at 695.817 kb on + strandat 695.818 kb on - strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.856 kb on + strandat 695.857 kb on - strandat 695.857 kb on - strandat 695.857 kb on - strandat 695.857 kb on - strandat 695.857 kb on - strandat 696.308 kb on + strand, within Pf6N2E2_612at 696.309 kb on - strand, within Pf6N2E2_612at 696.486 kb on + strand, within Pf6N2E2_612at 696.735 kb on - strand, within Pf6N2E2_612

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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693,865 - Pf6N2E2_610 0.44 -1.2
694,027 - Pf6N2E2_610 0.56 -0.4
694,134 + Pf6N2E2_610 0.64 -1.1
694,134 + Pf6N2E2_610 0.64 +0.6
694,134 + Pf6N2E2_610 0.64 +0.6
694,135 - Pf6N2E2_610 0.64 +0.0
694,218 + Pf6N2E2_610 0.71 +0.3
694,218 + Pf6N2E2_610 0.71 -0.6
694,218 + Pf6N2E2_610 0.71 +0.1
694,219 - Pf6N2E2_610 0.71 -0.4
694,219 - Pf6N2E2_610 0.71 -1.4
694,222 + Pf6N2E2_610 0.71 -1.4
694,222 + Pf6N2E2_610 0.71 +0.4
694,222 + Pf6N2E2_610 0.71 +0.3
694,222 + Pf6N2E2_610 0.71 +0.9
694,222 + Pf6N2E2_610 0.71 -0.8
694,223 - Pf6N2E2_610 0.71 -1.4
694,416 + Pf6N2E2_610 0.85 -1.4
694,452 + Pf6N2E2_610 0.88 -0.0
694,479 + +0.6
694,480 - -0.4
694,601 + -1.5
694,796 + +0.0
694,796 + +0.5
694,797 - -1.1
694,834 + -0.4
694,835 - +1.5
694,835 - -0.7
695,007 + Pf6N2E2_611 0.18 -0.4
695,245 - Pf6N2E2_611 0.42 +0.4
695,343 + Pf6N2E2_611 0.52 +1.4
695,343 + -1.0
695,343 + Pf6N2E2_611 0.52 -0.1
695,343 + Pf6N2E2_611 0.52 -0.2
695,344 - Pf6N2E2_611 0.52 -0.4
695,344 - Pf6N2E2_611 0.52 -0.3
695,430 + Pf6N2E2_611 0.61 -1.4
695,430 + Pf6N2E2_611 0.61 +0.9
695,431 - Pf6N2E2_611 0.61 -1.4
695,431 - Pf6N2E2_611 0.61 -0.9
695,431 - Pf6N2E2_611 0.61 -0.7
695,436 + Pf6N2E2_611 0.61 +0.6
695,625 + Pf6N2E2_611 0.80 -2.7
695,626 - Pf6N2E2_611 0.80 -1.3
695,817 + -1.8
695,818 - -0.2
695,856 + -0.7
695,856 + -1.0
695,856 + +0.8
695,856 + -1.8
695,856 + +1.0
695,856 + +1.3
695,856 + +1.7
695,856 + -0.4
695,857 - -0.7
695,857 - +0.2
695,857 - -0.2
695,857 - -0.2
695,857 - +0.5
696,308 + Pf6N2E2_612 0.30 +0.5
696,309 - Pf6N2E2_612 0.30 +0.3
696,486 + Pf6N2E2_612 0.41 -0.4
696,735 - Pf6N2E2_612 0.57 -0.4

Or see this region's nucleotide sequence