Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5773 and Pf6N2E2_5774 overlap by 4 nucleotides Pf6N2E2_5774 and Pf6N2E2_5775 are separated by 0 nucleotides Pf6N2E2_5775 and Pf6N2E2_5776 are separated by 29 nucleotides Pf6N2E2_5776 and Pf6N2E2_5777 are separated by 45 nucleotides Pf6N2E2_5777 and Pf6N2E2_5778 are separated by 17 nucleotides Pf6N2E2_5778 and Pf6N2E2_5779 are separated by 470 nucleotides
Pf6N2E2_5773 - Transcriptional regulator, Cro/CI family, at 6,589,738 to 6,590,103
_5773
Pf6N2E2_5774 - FIG00954549: hypothetical protein, at 6,590,100 to 6,590,471
_5774
Pf6N2E2_5775 - FOG: TPR repeat, SEL1 subfamily, at 6,590,472 to 6,590,873
_5775
Pf6N2E2_5776 - hypothetical protein, at 6,590,903 to 6,591,016
_5776
Pf6N2E2_5777 - Rhs family protein, at 6,591,062 to 6,595,720
_5777
Pf6N2E2_5778 - hypothetical protein, at 6,595,738 to 6,595,857
_5778
Pf6N2E2_5779 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 6,596,328 to 6,598,115
_5779
Position (kb)
6591
6592
6593
6594
6595
6596 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6590.107 kb on + strand at 6590.107 kb on + strand at 6590.107 kb on + strand at 6590.108 kb on - strand at 6590.176 kb on + strand, within Pf6N2E2_5774 at 6590.176 kb on + strand, within Pf6N2E2_5774 at 6590.176 kb on + strand, within Pf6N2E2_5774 at 6590.176 kb on + strand, within Pf6N2E2_5774 at 6590.177 kb on - strand, within Pf6N2E2_5774 at 6590.177 kb on - strand, within Pf6N2E2_5774 at 6590.177 kb on - strand, within Pf6N2E2_5774 at 6590.184 kb on + strand, within Pf6N2E2_5774 at 6590.185 kb on - strand, within Pf6N2E2_5774 at 6590.356 kb on + strand, within Pf6N2E2_5774 at 6590.356 kb on + strand, within Pf6N2E2_5774 at 6590.357 kb on - strand, within Pf6N2E2_5774 at 6590.357 kb on - strand, within Pf6N2E2_5774 at 6590.442 kb on + strand at 6590.443 kb on - strand at 6590.443 kb on - strand at 6590.443 kb on - strand at 6590.625 kb on + strand, within Pf6N2E2_5775 at 6590.625 kb on + strand, within Pf6N2E2_5775 at 6590.626 kb on - strand, within Pf6N2E2_5775 at 6590.626 kb on - strand, within Pf6N2E2_5775 at 6590.626 kb on - strand, within Pf6N2E2_5775 at 6590.634 kb on - strand, within Pf6N2E2_5775 at 6590.634 kb on - strand, within Pf6N2E2_5775 at 6590.866 kb on - strand at 6590.872 kb on - strand at 6590.915 kb on - strand, within Pf6N2E2_5776 at 6590.954 kb on + strand, within Pf6N2E2_5776 at 6590.954 kb on + strand, within Pf6N2E2_5776 at 6590.955 kb on - strand, within Pf6N2E2_5776 at 6590.955 kb on - strand, within Pf6N2E2_5776 at 6590.955 kb on - strand, within Pf6N2E2_5776 at 6590.955 kb on - strand, within Pf6N2E2_5776 at 6591.122 kb on + strand at 6591.290 kb on - strand at 6591.332 kb on + strand at 6591.332 kb on + strand at 6591.332 kb on + strand at 6591.333 kb on - strand at 6591.453 kb on - strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.512 kb on + strand at 6591.854 kb on - strand, within Pf6N2E2_5777 at 6591.854 kb on - strand, within Pf6N2E2_5777 at 6591.854 kb on - strand, within Pf6N2E2_5777 at 6591.980 kb on + strand, within Pf6N2E2_5777 at 6591.980 kb on + strand, within Pf6N2E2_5777 at 6591.980 kb on + strand, within Pf6N2E2_5777 at 6591.981 kb on - strand, within Pf6N2E2_5777 at 6592.079 kb on + strand, within Pf6N2E2_5777 at 6592.080 kb on - strand, within Pf6N2E2_5777 at 6592.080 kb on - strand, within Pf6N2E2_5777 at 6592.091 kb on + strand, within Pf6N2E2_5777 at 6592.551 kb on - strand, within Pf6N2E2_5777 at 6592.551 kb on - strand, within Pf6N2E2_5777 at 6592.551 kb on - strand, within Pf6N2E2_5777 at 6592.959 kb on - strand, within Pf6N2E2_5777 at 6593.036 kb on + strand, within Pf6N2E2_5777 at 6593.097 kb on - strand, within Pf6N2E2_5777 at 6593.097 kb on - strand, within Pf6N2E2_5777 at 6593.097 kb on - strand, within Pf6N2E2_5777 at 6593.097 kb on - strand, within Pf6N2E2_5777 at 6593.097 kb on - strand, within Pf6N2E2_5777 at 6593.097 kb on - strand, within Pf6N2E2_5777 at 6593.097 kb on - strand, within Pf6N2E2_5777 at 6593.097 kb on - strand, within Pf6N2E2_5777 at 6593.097 kb on - strand, within Pf6N2E2_5777 at 6593.292 kb on - strand, within Pf6N2E2_5777 at 6593.406 kb on - strand, within Pf6N2E2_5777 at 6593.406 kb on - strand, within Pf6N2E2_5777 at 6593.406 kb on - strand, within Pf6N2E2_5777 at 6594.903 kb on - strand, within Pf6N2E2_5777 at 6594.903 kb on - strand, within Pf6N2E2_5777 at 6594.903 kb on - strand, within Pf6N2E2_5777 at 6595.050 kb on - strand, within Pf6N2E2_5777 at 6595.050 kb on - strand, within Pf6N2E2_5777 at 6595.050 kb on - strand, within Pf6N2E2_5777 at 6595.157 kb on + strand, within Pf6N2E2_5777 at 6595.157 kb on + strand, within Pf6N2E2_5777 at 6595.157 kb on + strand, within Pf6N2E2_5777 at 6595.157 kb on + strand, within Pf6N2E2_5777 at 6595.172 kb on + strand, within Pf6N2E2_5777 at 6595.175 kb on + strand, within Pf6N2E2_5777 at 6595.175 kb on + strand, within Pf6N2E2_5777 at 6595.175 kb on + strand, within Pf6N2E2_5777 at 6595.175 kb on + strand, within Pf6N2E2_5777 at 6595.399 kb on + strand at 6595.399 kb on + strand at 6595.399 kb on + strand at 6595.400 kb on - strand at 6595.400 kb on - strand at 6595.400 kb on - strand at 6595.405 kb on + strand at 6595.405 kb on + strand at 6595.405 kb on + strand at 6595.406 kb on - strand at 6595.450 kb on + strand at 6595.451 kb on - strand at 6595.546 kb on - strand at 6595.550 kb on - strand at 6595.552 kb on + strand at 6595.562 kb on - strand at 6595.573 kb on + strand at 6595.573 kb on + strand at 6595.573 kb on + strand at 6595.574 kb on - strand at 6595.579 kb on + strand at 6595.579 kb on + strand at 6595.612 kb on - strand at 6595.716 kb on + strand at 6595.716 kb on + strand at 6595.716 kb on + strand at 6595.717 kb on - strand at 6595.739 kb on + strand at 6595.851 kb on + strand at 6595.874 kb on + strand at 6595.912 kb on + strand at 6595.916 kb on + strand at 6595.936 kb on + strand at 6595.939 kb on + strand at 6595.939 kb on + strand at 6595.939 kb on + strand at 6595.939 kb on + strand at 6595.945 kb on + strand at 6595.979 kb on + strand at 6595.979 kb on + strand at 6596.007 kb on + strand at 6596.008 kb on - strand at 6596.079 kb on + strand at 6596.079 kb on + strand at 6596.080 kb on - strand at 6596.080 kb on - strand at 6596.104 kb on + strand at 6596.104 kb on + strand at 6596.105 kb on - strand at 6596.198 kb on + strand at 6596.199 kb on - strand at 6596.199 kb on - strand at 6596.199 kb on - strand at 6596.216 kb on - strand at 6596.330 kb on - strand at 6596.330 kb on - strand at 6596.386 kb on + strand at 6596.386 kb on + strand at 6596.387 kb on - strand at 6596.387 kb on - strand at 6596.387 kb on - strand at 6596.387 kb on - strand at 6596.534 kb on + strand, within Pf6N2E2_5779 at 6596.555 kb on + strand, within Pf6N2E2_5779 at 6596.572 kb on - strand, within Pf6N2E2_5779 at 6596.572 kb on - strand, within Pf6N2E2_5779 at 6596.587 kb on + strand, within Pf6N2E2_5779
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 6,590,107 + -0.4 6,590,107 + +0.1 6,590,107 + -0.7 6,590,108 - +1.8 6,590,176 + Pf6N2E2_5774 0.20 -0.6 6,590,176 + Pf6N2E2_5774 0.20 +1.4 6,590,176 + Pf6N2E2_5774 0.20 +1.7 6,590,176 + Pf6N2E2_5774 0.20 +0.7 6,590,177 - Pf6N2E2_5774 0.21 -0.9 6,590,177 - Pf6N2E2_5774 0.21 -0.1 6,590,177 - Pf6N2E2_5774 0.21 +1.3 6,590,184 + Pf6N2E2_5774 0.23 +0.0 6,590,185 - Pf6N2E2_5774 0.23 +2.6 6,590,356 + Pf6N2E2_5774 0.69 -0.6 6,590,356 + Pf6N2E2_5774 0.69 -1.1 6,590,357 - Pf6N2E2_5774 0.69 +1.6 6,590,357 - Pf6N2E2_5774 0.69 +1.3 6,590,442 + -0.9 6,590,443 - -0.8 6,590,443 - -0.5 6,590,443 - +0.6 6,590,625 + Pf6N2E2_5775 0.38 +0.5 6,590,625 + Pf6N2E2_5775 0.38 +1.8 6,590,626 - Pf6N2E2_5775 0.38 +3.3 6,590,626 - Pf6N2E2_5775 0.38 +1.3 6,590,626 - Pf6N2E2_5775 0.38 +0.5 6,590,634 - Pf6N2E2_5775 0.40 -0.3 6,590,634 - Pf6N2E2_5775 0.40 -0.9 6,590,866 - +0.7 6,590,872 - +0.1 6,590,915 - Pf6N2E2_5776 0.11 +0.1 6,590,954 + Pf6N2E2_5776 0.45 -0.5 6,590,954 + Pf6N2E2_5776 0.45 -1.6 6,590,955 - Pf6N2E2_5776 0.46 +0.4 6,590,955 - Pf6N2E2_5776 0.46 -2.1 6,590,955 - Pf6N2E2_5776 0.46 +0.1 6,590,955 - Pf6N2E2_5776 0.46 -0.2 6,591,122 + +0.3 6,591,290 - +0.3 6,591,332 + -0.3 6,591,332 + +0.1 6,591,332 + +0.2 6,591,333 - -0.7 6,591,453 - +0.7 6,591,512 + +0.3 6,591,512 + +0.8 6,591,512 + +0.4 6,591,512 + +1.3 6,591,512 + -0.4 6,591,512 + +0.1 6,591,854 - Pf6N2E2_5777 0.17 +1.3 6,591,854 - Pf6N2E2_5777 0.17 -0.5 6,591,854 - Pf6N2E2_5777 0.17 -1.6 6,591,980 + Pf6N2E2_5777 0.20 -0.3 6,591,980 + Pf6N2E2_5777 0.20 -0.3 6,591,980 + Pf6N2E2_5777 0.20 +1.4 6,591,981 - Pf6N2E2_5777 0.20 -1.3 6,592,079 + Pf6N2E2_5777 0.22 +1.3 6,592,080 - Pf6N2E2_5777 0.22 -1.1 6,592,080 - Pf6N2E2_5777 0.22 +0.8 6,592,091 + Pf6N2E2_5777 0.22 -0.6 6,592,551 - Pf6N2E2_5777 0.32 +0.5 6,592,551 - Pf6N2E2_5777 0.32 -0.3 6,592,551 - Pf6N2E2_5777 0.32 -0.2 6,592,959 - Pf6N2E2_5777 0.41 +0.3 6,593,036 + Pf6N2E2_5777 0.42 -1.4 6,593,097 - Pf6N2E2_5777 0.44 +0.7 6,593,097 - Pf6N2E2_5777 0.44 +0.7 6,593,097 - Pf6N2E2_5777 0.44 +0.7 6,593,097 - Pf6N2E2_5777 0.44 -0.7 6,593,097 - Pf6N2E2_5777 0.44 +1.2 6,593,097 - Pf6N2E2_5777 0.44 -1.2 6,593,097 - Pf6N2E2_5777 0.44 -2.1 6,593,097 - Pf6N2E2_5777 0.44 -1.7 6,593,097 - Pf6N2E2_5777 0.44 -0.8 6,593,292 - Pf6N2E2_5777 0.48 -1.1 6,593,406 - Pf6N2E2_5777 0.50 -0.1 6,593,406 - Pf6N2E2_5777 0.50 -0.6 6,593,406 - Pf6N2E2_5777 0.50 +0.2 6,594,903 - Pf6N2E2_5777 0.82 +2.2 6,594,903 - Pf6N2E2_5777 0.82 -0.8 6,594,903 - Pf6N2E2_5777 0.82 +0.8 6,595,050 - Pf6N2E2_5777 0.86 -2.0 6,595,050 - Pf6N2E2_5777 0.86 +0.9 6,595,050 - Pf6N2E2_5777 0.86 -1.1 6,595,157 + Pf6N2E2_5777 0.88 -1.1 6,595,157 + Pf6N2E2_5777 0.88 -0.5 6,595,157 + Pf6N2E2_5777 0.88 +0.5 6,595,157 + Pf6N2E2_5777 0.88 -0.3 6,595,172 + Pf6N2E2_5777 0.88 +1.8 6,595,175 + Pf6N2E2_5777 0.88 +0.4 6,595,175 + Pf6N2E2_5777 0.88 +0.2 6,595,175 + Pf6N2E2_5777 0.88 -0.1 6,595,175 + Pf6N2E2_5777 0.88 +0.0 6,595,399 + -1.9 6,595,399 + -0.5 6,595,399 + +0.2 6,595,400 - +0.0 6,595,400 - +0.1 6,595,400 - +0.0 6,595,405 + -0.8 6,595,405 + -2.9 6,595,405 + -1.1 6,595,406 - +1.2 6,595,450 + +0.3 6,595,451 - +0.2 6,595,546 - +2.2 6,595,550 - +0.2 6,595,552 + -1.1 6,595,562 - +1.0 6,595,573 + +0.2 6,595,573 + +1.2 6,595,573 + +1.4 6,595,574 - +0.2 6,595,579 + +1.4 6,595,579 + -1.9 6,595,612 - -0.3 6,595,716 + +1.7 6,595,716 + +1.1 6,595,716 + +0.2 6,595,717 - -0.3 6,595,739 + -0.6 6,595,851 + -0.7 6,595,874 + -0.8 6,595,912 + +0.2 6,595,916 + -2.5 6,595,936 + -2.5 6,595,939 + +0.4 6,595,939 + +0.9 6,595,939 + +1.0 6,595,939 + +0.3 6,595,945 + -2.3 6,595,979 + +0.8 6,595,979 + +0.3 6,596,007 + +0.2 6,596,008 - -1.1 6,596,079 + +0.5 6,596,079 + -0.0 6,596,080 - -0.3 6,596,080 - +2.2 6,596,104 + -0.9 6,596,104 + +1.3 6,596,105 - -2.9 6,596,198 + -0.9 6,596,199 - +0.8 6,596,199 - -0.3 6,596,199 - +0.2 6,596,216 - -0.7 6,596,330 - -2.2 6,596,330 - -0.9 6,596,386 + -0.5 6,596,386 + +0.1 6,596,387 - -0.3 6,596,387 - +2.1 6,596,387 - +0.7 6,596,387 - -0.6 6,596,534 + Pf6N2E2_5779 0.12 -0.9 6,596,555 + Pf6N2E2_5779 0.13 +1.0 6,596,572 - Pf6N2E2_5779 0.14 +0.7 6,596,572 - Pf6N2E2_5779 0.14 +0.7 6,596,587 + Pf6N2E2_5779 0.14 -0.1
Or see this region's nucleotide sequence