Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16170 and BWI76_RS16175 are separated by 111 nucleotides BWI76_RS16175 and BWI76_RS16180 are separated by 453 nucleotides
BWI76_RS16170: BWI76_RS16170 - amidohydrolase, at 3,354,337 to 3,355,782
_RS16170
BWI76_RS16175: BWI76_RS16175 - p-aminobenzoyl-glutamate transporter, at 3,355,894 to 3,357,420
_RS16175
BWI76_RS16180: BWI76_RS16180 - methylated-DNA--protein-cysteine methyltransferase, at 3,357,874 to 3,358,389
_RS16180
Position (kb)
3355
3356
3357
3358 Strain fitness (log2 ratio)
-2
-1
0
1 at 3355.081 kb on + strand, within BWI76_RS16170 at 3355.197 kb on - strand, within BWI76_RS16170 at 3355.441 kb on - strand, within BWI76_RS16170 at 3355.724 kb on - strand at 3355.724 kb on - strand at 3355.766 kb on - strand at 3355.905 kb on + strand at 3355.912 kb on + strand at 3355.943 kb on - strand at 3356.652 kb on + strand, within BWI76_RS16175 at 3356.652 kb on + strand, within BWI76_RS16175 at 3356.677 kb on - strand, within BWI76_RS16175 at 3356.680 kb on + strand, within BWI76_RS16175 at 3357.431 kb on + strand at 3357.603 kb on - strand at 3357.644 kb on + strand at 3357.686 kb on + strand at 3357.899 kb on - strand at 3358.274 kb on + strand, within BWI76_RS16180 at 3358.344 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,355,081 + BWI76_RS16170 0.51 +0.7 3,355,197 - BWI76_RS16170 0.59 -2.1 3,355,441 - BWI76_RS16170 0.76 +0.7 3,355,724 - +0.4 3,355,724 - +1.0 3,355,766 - +0.1 3,355,905 + -0.5 3,355,912 + +0.5 3,355,943 - -0.8 3,356,652 + BWI76_RS16175 0.50 +1.1 3,356,652 + BWI76_RS16175 0.50 +1.1 3,356,677 - BWI76_RS16175 0.51 +0.4 3,356,680 + BWI76_RS16175 0.51 -0.1 3,357,431 + -1.1 3,357,603 - -0.5 3,357,644 + +0.4 3,357,686 + +0.6 3,357,899 - -1.3 3,358,274 + BWI76_RS16180 0.78 -0.6 3,358,344 + -0.3
Or see this region's nucleotide sequence