Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13215 and BWI76_RS13220 are separated by 243 nucleotides BWI76_RS13220 and BWI76_RS13225 are separated by 86 nucleotides BWI76_RS13225 and BWI76_RS13230 are separated by 132 nucleotides
BWI76_RS13215: BWI76_RS13215 - type I glyceraldehyde-3-phosphate dehydrogenase, at 2,758,236 to 2,759,234
_RS13215
BWI76_RS13220: BWI76_RS13220 - N-acetyltransferase, at 2,759,478 to 2,759,972
_RS13220
BWI76_RS13225: BWI76_RS13225 - hypothetical protein, at 2,760,059 to 2,760,985
_RS13225
BWI76_RS13230: BWI76_RS13230 - GGDEF domain-containing protein, at 2,761,118 to 2,762,089
_RS13230
Position (kb)
2760
2761 Strain fitness (log2 ratio)
-1
0
1 at 2759.150 kb on + strand at 2759.150 kb on + strand at 2759.153 kb on + strand at 2759.158 kb on - strand at 2759.204 kb on + strand at 2759.454 kb on + strand at 2759.524 kb on - strand at 2759.681 kb on - strand, within BWI76_RS13220 at 2759.835 kb on + strand, within BWI76_RS13220 at 2760.021 kb on - strand at 2760.034 kb on - strand at 2760.103 kb on - strand at 2760.287 kb on + strand, within BWI76_RS13225 at 2760.622 kb on + strand, within BWI76_RS13225 at 2760.635 kb on - strand, within BWI76_RS13225 at 2760.796 kb on + strand, within BWI76_RS13225 at 2760.927 kb on + strand at 2761.278 kb on + strand, within BWI76_RS13230 at 2761.776 kb on - strand, within BWI76_RS13230
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,759,150 + +1.4 2,759,150 + +0.3 2,759,153 + +0.2 2,759,158 - -0.1 2,759,204 + +0.6 2,759,454 + -0.4 2,759,524 - +0.3 2,759,681 - BWI76_RS13220 0.41 -0.1 2,759,835 + BWI76_RS13220 0.72 +0.1 2,760,021 - +0.1 2,760,034 - -0.0 2,760,103 - +1.1 2,760,287 + BWI76_RS13225 0.25 +0.5 2,760,622 + BWI76_RS13225 0.61 +0.9 2,760,635 - BWI76_RS13225 0.62 +1.0 2,760,796 + BWI76_RS13225 0.80 -0.1 2,760,927 + +0.4 2,761,278 + BWI76_RS13230 0.16 +0.0 2,761,776 - BWI76_RS13230 0.68 +0.3
Or see this region's nucleotide sequence