Experiment: RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07850 and BWI76_RS07855 overlap by 4 nucleotides BWI76_RS07855 and BWI76_RS07860 are separated by 2 nucleotides
BWI76_RS07850: BWI76_RS07850 - putative cytosine deaminase-related metal-dependent hydrolase, at 1,665,150 to 1,666,388
_RS07850
BWI76_RS07855: BWI76_RS07855 - PucR family transcriptional regulator, at 1,666,385 to 1,667,620
_RS07855
BWI76_RS07860: BWI76_RS07860 - cytosine permease, at 1,667,623 to 1,668,885
_RS07860
Position (kb)
1666
1667
1668 Strain fitness (log2 ratio)
-1
0
1
2 at 1665.432 kb on - strand, within BWI76_RS07850 at 1665.631 kb on - strand, within BWI76_RS07850 at 1665.681 kb on - strand, within BWI76_RS07850 at 1665.762 kb on + strand, within BWI76_RS07850 at 1665.921 kb on + strand, within BWI76_RS07850 at 1666.045 kb on + strand, within BWI76_RS07850 at 1666.548 kb on + strand, within BWI76_RS07855 at 1666.669 kb on + strand at 1667.291 kb on - strand, within BWI76_RS07855 at 1667.622 kb on - strand at 1667.700 kb on + strand at 1667.700 kb on + strand at 1667.700 kb on + strand at 1667.887 kb on - strand, within BWI76_RS07860 at 1668.228 kb on + strand, within BWI76_RS07860 at 1668.508 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Methylobacterium sp. UNC378MF 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,665,432 - BWI76_RS07850 0.23 +0.7 1,665,631 - BWI76_RS07850 0.39 -0.3 1,665,681 - BWI76_RS07850 0.43 +1.1 1,665,762 + BWI76_RS07850 0.49 +0.4 1,665,921 + BWI76_RS07850 0.62 +0.8 1,666,045 + BWI76_RS07850 0.72 -0.4 1,666,548 + BWI76_RS07855 0.13 +0.9 1,666,669 + +1.6 1,667,291 - BWI76_RS07855 0.73 +0.4 1,667,622 - -0.9 1,667,700 + +0.6 1,667,700 + +0.4 1,667,700 + +0.4 1,667,887 - BWI76_RS07860 0.21 +0.3 1,668,228 + BWI76_RS07860 0.48 -0.2 1,668,508 - +2.2
Or see this region's nucleotide sequence