Experiment: Ferric citrate electron acceptor; flight; DNA cleaned with chelator
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1447 and SO1448 are separated by 79 nucleotides SO1448 and SO1449 are separated by 23 nucleotides SO1449 and SO1450 overlap by 14 nucleotides SO1450 and SO1451 are separated by 186 nucleotides SO1451 and SO1452 overlap by 17 nucleotides SO1452 and SO1453 overlap by 11 nucleotides
SO1447: SO1447 - retinol acyltransferase domain protein (NCBI ptt file), at 1,508,397 to 1,509,119
SO1447
SO1448: SO1448 - site-specific recombinase, phage integrase family (NCBI ptt file), at 1,509,199 to 1,509,765
SO1448
SO1449: SO1449 - hypothetical protein (NCBI ptt file), at 1,509,789 to 1,509,887
SO1449
SO1450: SO1450 - conserved hypothetical protein (NCBI ptt file), at 1,509,874 to 1,510,281
SO1450
SO1451: SO1451 - conserved hypothetical protein (NCBI ptt file), at 1,510,468 to 1,510,881
SO1451
SO1452: SO1452 - hypothetical protein (NCBI ptt file), at 1,510,865 to 1,511,077
SO1452
SO1453: SO1453 - conserved hypothetical protein (NCBI ptt file), at 1,511,067 to 1,511,441
SO1453
Position (kb)
1509
1510
1511 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1508.886 kb on + strand, within SO1447 at 1508.886 kb on + strand, within SO1447 at 1508.912 kb on + strand, within SO1447 at 1508.992 kb on + strand, within SO1447 at 1509.003 kb on + strand, within SO1447 at 1509.016 kb on - strand, within SO1447 at 1509.016 kb on - strand, within SO1447 at 1509.038 kb on - strand, within SO1447 at 1509.049 kb on - strand at 1509.049 kb on - strand at 1509.159 kb on - strand at 1509.159 kb on - strand at 1509.181 kb on - strand at 1509.301 kb on - strand, within SO1448 at 1509.327 kb on - strand, within SO1448 at 1509.330 kb on + strand, within SO1448 at 1509.343 kb on + strand, within SO1448 at 1509.484 kb on - strand, within SO1448 at 1509.503 kb on - strand, within SO1448 at 1509.654 kb on + strand, within SO1448 at 1509.654 kb on + strand, within SO1448 at 1509.654 kb on + strand, within SO1448 at 1509.654 kb on + strand, within SO1448 at 1509.654 kb on + strand, within SO1448 at 1509.662 kb on - strand, within SO1448 at 1509.685 kb on + strand, within SO1448 at 1509.739 kb on + strand at 1509.739 kb on + strand at 1509.747 kb on - strand at 1509.772 kb on - strand at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.881 kb on + strand at 1509.889 kb on - strand at 1509.999 kb on - strand, within SO1450 at 1510.038 kb on + strand, within SO1450 at 1510.136 kb on - strand, within SO1450 at 1510.144 kb on + strand, within SO1450 at 1510.186 kb on + strand, within SO1450 at 1510.186 kb on + strand, within SO1450 at 1510.191 kb on + strand, within SO1450 at 1510.191 kb on + strand, within SO1450 at 1510.191 kb on + strand, within SO1450 at 1510.194 kb on - strand, within SO1450 at 1510.194 kb on - strand, within SO1450 at 1510.194 kb on - strand, within SO1450 at 1510.194 kb on - strand, within SO1450 at 1510.265 kb on - strand at 1510.376 kb on + strand at 1510.384 kb on - strand at 1510.480 kb on + strand at 1510.563 kb on + strand, within SO1451 at 1510.586 kb on + strand, within SO1451 at 1510.586 kb on + strand, within SO1451 at 1510.594 kb on - strand, within SO1451 at 1510.594 kb on - strand, within SO1451 at 1510.594 kb on - strand, within SO1451 at 1510.594 kb on - strand, within SO1451 at 1510.594 kb on - strand, within SO1451 at 1510.622 kb on + strand, within SO1451 at 1510.622 kb on + strand, within SO1451 at 1510.637 kb on + strand, within SO1451 at 1510.742 kb on + strand, within SO1451 at 1510.920 kb on - strand, within SO1452 at 1510.976 kb on + strand, within SO1452 at 1511.004 kb on + strand, within SO1452 at 1511.004 kb on + strand, within SO1452 at 1511.047 kb on + strand, within SO1452 at 1511.055 kb on - strand, within SO1452 at 1511.063 kb on - strand at 1511.066 kb on + strand at 1511.085 kb on - strand at 1511.169 kb on + strand, within SO1453 at 1511.169 kb on + strand, within SO1453 at 1511.169 kb on + strand, within SO1453 at 1511.177 kb on - strand, within SO1453 at 1511.177 kb on - strand, within SO1453 at 1511.207 kb on - strand, within SO1453 at 1511.207 kb on - strand, within SO1453 at 1511.207 kb on - strand, within SO1453
Per-strain Table
Position Strand Gene LocusTag Fraction Ferric citrate electron acceptor; flight; DNA cleaned with chelator remove 1,508,886 + SO1447 0.68 -0.9 1,508,886 + SO1447 0.68 -0.2 1,508,912 + SO1447 0.71 +2.7 1,508,992 + SO1447 0.82 -0.9 1,509,003 + SO1447 0.84 -2.3 1,509,016 - SO1447 0.86 +0.5 1,509,016 - SO1447 0.86 -1.1 1,509,038 - SO1447 0.89 -0.3 1,509,049 - -2.3 1,509,049 - -0.1 1,509,159 - +1.6 1,509,159 - -0.4 1,509,181 - -1.2 1,509,301 - SO1448 0.18 -0.9 1,509,327 - SO1448 0.23 +1.8 1,509,330 + SO1448 0.23 -1.3 1,509,343 + SO1448 0.25 -1.1 1,509,484 - SO1448 0.50 -0.2 1,509,503 - SO1448 0.54 +0.6 1,509,654 + SO1448 0.80 -0.6 1,509,654 + SO1448 0.80 -1.2 1,509,654 + SO1448 0.80 +0.5 1,509,654 + SO1448 0.80 -1.9 1,509,654 + SO1448 0.80 +0.1 1,509,662 - SO1448 0.82 +2.2 1,509,685 + SO1448 0.86 +0.6 1,509,739 + +1.2 1,509,739 + +0.4 1,509,747 - +0.7 1,509,772 - -1.1 1,509,881 + -1.9 1,509,881 + +0.2 1,509,881 + +1.1 1,509,889 - +0.2 1,509,999 - SO1450 0.31 -0.6 1,510,038 + SO1450 0.40 -0.9 1,510,136 - SO1450 0.64 -1.3 1,510,144 + SO1450 0.66 -0.8 1,510,186 + SO1450 0.76 -1.3 1,510,186 + SO1450 0.76 -0.6 1,510,191 + SO1450 0.78 -1.3 1,510,191 + SO1450 0.78 -1.6 1,510,191 + SO1450 0.78 -2.1 1,510,194 - SO1450 0.78 -4.8 1,510,194 - SO1450 0.78 -0.9 1,510,194 - SO1450 0.78 -0.7 1,510,194 - SO1450 0.78 -0.6 1,510,265 - -0.9 1,510,376 + -0.9 1,510,384 - +1.2 1,510,480 + -0.9 1,510,563 + SO1451 0.23 -0.4 1,510,586 + SO1451 0.29 -2.1 1,510,586 + SO1451 0.29 +0.7 1,510,594 - SO1451 0.30 -2.5 1,510,594 - SO1451 0.30 +1.4 1,510,594 - SO1451 0.30 +0.7 1,510,594 - SO1451 0.30 +0.8 1,510,594 - SO1451 0.30 -0.4 1,510,622 + SO1451 0.37 -1.8 1,510,622 + SO1451 0.37 +0.6 1,510,637 + SO1451 0.41 -2.2 1,510,742 + SO1451 0.66 -1.2 1,510,920 - SO1452 0.26 -0.2 1,510,976 + SO1452 0.52 -0.5 1,511,004 + SO1452 0.65 -0.1 1,511,004 + SO1452 0.65 +0.2 1,511,047 + SO1452 0.85 -0.9 1,511,055 - SO1452 0.89 -0.3 1,511,063 - -1.5 1,511,066 + +1.9 1,511,085 - -0.9 1,511,169 + SO1453 0.27 -0.8 1,511,169 + SO1453 0.27 +0.8 1,511,169 + SO1453 0.27 -1.5 1,511,177 - SO1453 0.29 -1.0 1,511,177 - SO1453 0.29 -0.2 1,511,207 - SO1453 0.37 -0.7 1,511,207 - SO1453 0.37 +0.8 1,511,207 - SO1453 0.37 -0.1
Or see this region's nucleotide sequence