Experiment: MoLS4 with Formamide 10mM (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tpX and DVU1230 are separated by 195 nucleotides DVU1230 and amt overlap by 4 nucleotides amt and glnB-1 are separated by 122 nucleotides glnB-1 and glnD are separated by 55 nucleotides
DVU1228: tpX - thiol peroxidase (TIGR), at 1,317,780 to 1,318,298
tpX
DVU1230: DVU1230 - hypothetical protein (TIGR), at 1,318,494 to 1,318,604
DVU1230
DVU1231: amt - ammonium transporter (TIGR), at 1,318,601 to 1,319,809
amt
DVU1232: glnB-1 - nitrogen regulatory protein P-II (TIGR), at 1,319,932 to 1,320,270
glnB-1
DVU1233: glnD - protein-P-II uridylyltransferase, putative (TIGR), at 1,320,326 to 1,323,046
glnD
Position (kb)
1318
1319
1320 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1317.656 kb on - strand at 1317.699 kb on - strand at 1317.841 kb on + strand, within tpX at 1317.927 kb on + strand, within tpX at 1317.936 kb on - strand, within tpX at 1317.953 kb on + strand, within tpX at 1317.957 kb on - strand, within tpX at 1317.960 kb on + strand, within tpX at 1318.055 kb on - strand, within tpX at 1318.057 kb on + strand, within tpX at 1318.123 kb on - strand, within tpX at 1318.169 kb on - strand, within tpX at 1318.221 kb on + strand, within tpX at 1318.236 kb on - strand, within tpX at 1318.255 kb on - strand at 1318.309 kb on - strand at 1318.309 kb on - strand at 1318.358 kb on - strand at 1318.593 kb on - strand, within DVU1230 at 1318.603 kb on - strand at 1318.628 kb on - strand at 1318.670 kb on + strand at 1318.675 kb on + strand at 1318.675 kb on + strand at 1318.675 kb on + strand at 1318.683 kb on - strand at 1318.683 kb on - strand at 1318.683 kb on - strand at 1318.683 kb on - strand at 1318.683 kb on - strand at 1318.683 kb on - strand at 1318.690 kb on + strand at 1318.705 kb on - strand at 1318.711 kb on + strand at 1318.864 kb on - strand, within amt at 1318.883 kb on + strand, within amt at 1318.906 kb on - strand, within amt at 1318.929 kb on - strand, within amt at 1318.944 kb on - strand, within amt at 1318.984 kb on + strand, within amt at 1318.986 kb on + strand, within amt at 1319.045 kb on + strand, within amt at 1319.147 kb on + strand, within amt at 1319.361 kb on + strand, within amt at 1319.368 kb on + strand, within amt at 1319.564 kb on - strand, within amt at 1319.597 kb on + strand, within amt at 1319.605 kb on - strand, within amt at 1319.615 kb on + strand, within amt at 1319.653 kb on + strand, within amt at 1319.670 kb on + strand, within amt at 1319.683 kb on + strand, within amt at 1319.707 kb on + strand at 1319.715 kb on - strand at 1319.820 kb on - strand at 1319.883 kb on - strand at 1319.902 kb on + strand at 1319.924 kb on - strand at 1319.980 kb on + strand, within glnB-1 at 1320.057 kb on + strand, within glnB-1 at 1320.105 kb on + strand, within glnB-1 at 1320.105 kb on + strand, within glnB-1 at 1320.138 kb on + strand, within glnB-1 at 1320.146 kb on - strand, within glnB-1 at 1320.146 kb on - strand, within glnB-1 at 1320.162 kb on + strand, within glnB-1 at 1320.168 kb on - strand, within glnB-1 at 1320.379 kb on + strand at 1320.483 kb on - strand at 1320.506 kb on - strand at 1320.508 kb on + strand at 1320.518 kb on + strand at 1320.518 kb on + strand at 1320.518 kb on + strand at 1320.526 kb on - strand at 1320.568 kb on + strand at 1320.568 kb on + strand at 1320.568 kb on + strand at 1320.660 kb on - strand, within glnD at 1320.728 kb on + strand, within glnD at 1320.743 kb on + strand, within glnD at 1320.791 kb on - strand, within glnD
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with Formamide 10mM (N) remove 1,317,656 - -0.5 1,317,699 - +0.1 1,317,841 + tpX DVU1228 0.12 -0.1 1,317,927 + tpX DVU1228 0.28 -0.3 1,317,936 - tpX DVU1228 0.30 -0.1 1,317,953 + tpX DVU1228 0.33 -0.1 1,317,957 - tpX DVU1228 0.34 -1.3 1,317,960 + tpX DVU1228 0.35 -0.7 1,318,055 - tpX DVU1228 0.53 -0.3 1,318,057 + tpX DVU1228 0.53 -0.3 1,318,123 - tpX DVU1228 0.66 -0.4 1,318,169 - tpX DVU1228 0.75 -0.1 1,318,221 + tpX DVU1228 0.85 +0.9 1,318,236 - tpX DVU1228 0.88 -0.6 1,318,255 - -0.3 1,318,309 - -0.9 1,318,309 - -0.7 1,318,358 - -0.6 1,318,593 - DVU1230 0.89 -1.3 1,318,603 - -2.2 1,318,628 - -3.0 1,318,670 + -1.4 1,318,675 + -1.7 1,318,675 + -2.3 1,318,675 + +0.2 1,318,683 - -1.1 1,318,683 - -1.7 1,318,683 - -1.4 1,318,683 - -2.2 1,318,683 - -2.7 1,318,683 - -2.9 1,318,690 + -1.0 1,318,705 - -2.0 1,318,711 + +0.9 1,318,864 - amt DVU1231 0.22 -1.8 1,318,883 + amt DVU1231 0.23 -0.8 1,318,906 - amt DVU1231 0.25 -1.9 1,318,929 - amt DVU1231 0.27 -2.0 1,318,944 - amt DVU1231 0.28 -0.1 1,318,984 + amt DVU1231 0.32 -2.1 1,318,986 + amt DVU1231 0.32 -0.1 1,319,045 + amt DVU1231 0.37 -2.4 1,319,147 + amt DVU1231 0.45 -3.4 1,319,361 + amt DVU1231 0.63 -2.1 1,319,368 + amt DVU1231 0.63 -2.1 1,319,564 - amt DVU1231 0.80 -2.3 1,319,597 + amt DVU1231 0.82 -2.3 1,319,605 - amt DVU1231 0.83 -0.5 1,319,615 + amt DVU1231 0.84 -0.0 1,319,653 + amt DVU1231 0.87 -2.2 1,319,670 + amt DVU1231 0.88 -2.3 1,319,683 + amt DVU1231 0.89 -0.1 1,319,707 + -2.4 1,319,715 - -2.3 1,319,820 - +0.9 1,319,883 - -0.5 1,319,902 + -1.1 1,319,924 - -1.0 1,319,980 + glnB-1 DVU1232 0.14 -1.4 1,320,057 + glnB-1 DVU1232 0.37 -1.1 1,320,105 + glnB-1 DVU1232 0.51 +0.1 1,320,105 + glnB-1 DVU1232 0.51 -0.7 1,320,138 + glnB-1 DVU1232 0.61 -0.0 1,320,146 - glnB-1 DVU1232 0.63 -0.9 1,320,146 - glnB-1 DVU1232 0.63 -0.7 1,320,162 + glnB-1 DVU1232 0.68 -1.1 1,320,168 - glnB-1 DVU1232 0.70 -1.3 1,320,379 + +0.1 1,320,483 - +0.4 1,320,506 - -0.4 1,320,508 + -1.1 1,320,518 + +0.7 1,320,518 + +0.2 1,320,518 + +1.1 1,320,526 - +0.7 1,320,568 + +0.6 1,320,568 + +0.1 1,320,568 + +1.4 1,320,660 - glnD DVU1233 0.12 -1.9 1,320,728 + glnD DVU1233 0.15 -0.6 1,320,743 + glnD DVU1233 0.15 -0.1 1,320,791 - glnD DVU1233 0.17 +0.9
Or see this region's nucleotide sequence