Experiment: Acanthamoeba sp., muxed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS10320 and ABZR87_RS10325 are separated by 10 nucleotides ABZR87_RS10325 and ABZR87_RS10330 are separated by 93 nucleotides
ABZR87_RS10320: ABZR87_RS10320 - MFS transporter, at 2,186,940 to 2,188,232
_RS10320
ABZR87_RS10325: ABZR87_RS10325 - glycoside hydrolase family 31 protein, at 2,188,243 to 2,190,654
_RS10325
ABZR87_RS10330: ABZR87_RS10330 - porin, at 2,190,748 to 2,191,956
_RS10330
Position (kb)
2188
2189
2190
2191 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2187.421 kb on - strand, within ABZR87_RS10320 at 2187.421 kb on - strand, within ABZR87_RS10320 at 2187.492 kb on + strand, within ABZR87_RS10320 at 2187.493 kb on - strand, within ABZR87_RS10320 at 2187.493 kb on - strand, within ABZR87_RS10320 at 2187.493 kb on - strand, within ABZR87_RS10320 at 2187.693 kb on + strand, within ABZR87_RS10320 at 2187.694 kb on - strand, within ABZR87_RS10320 at 2187.694 kb on - strand, within ABZR87_RS10320 at 2187.789 kb on + strand, within ABZR87_RS10320 at 2187.913 kb on - strand, within ABZR87_RS10320 at 2188.633 kb on + strand, within ABZR87_RS10325 at 2188.634 kb on - strand, within ABZR87_RS10325 at 2188.646 kb on - strand, within ABZR87_RS10325 at 2188.663 kb on + strand, within ABZR87_RS10325 at 2188.669 kb on + strand, within ABZR87_RS10325 at 2188.669 kb on + strand, within ABZR87_RS10325 at 2188.669 kb on + strand, within ABZR87_RS10325 at 2188.670 kb on - strand, within ABZR87_RS10325 at 2188.670 kb on - strand, within ABZR87_RS10325 at 2188.670 kb on - strand, within ABZR87_RS10325 at 2188.696 kb on + strand, within ABZR87_RS10325 at 2188.696 kb on + strand, within ABZR87_RS10325 at 2188.697 kb on - strand, within ABZR87_RS10325 at 2188.747 kb on + strand, within ABZR87_RS10325 at 2188.805 kb on - strand, within ABZR87_RS10325 at 2188.805 kb on - strand, within ABZR87_RS10325 at 2188.822 kb on + strand, within ABZR87_RS10325 at 2188.867 kb on + strand, within ABZR87_RS10325 at 2188.868 kb on - strand, within ABZR87_RS10325 at 2188.914 kb on + strand, within ABZR87_RS10325 at 2188.914 kb on + strand, within ABZR87_RS10325 at 2188.914 kb on + strand, within ABZR87_RS10325 at 2188.915 kb on - strand, within ABZR87_RS10325 at 2188.918 kb on + strand, within ABZR87_RS10325 at 2188.918 kb on + strand, within ABZR87_RS10325 at 2188.918 kb on + strand, within ABZR87_RS10325 at 2188.918 kb on + strand, within ABZR87_RS10325 at 2188.919 kb on - strand, within ABZR87_RS10325 at 2188.919 kb on - strand, within ABZR87_RS10325 at 2188.919 kb on - strand, within ABZR87_RS10325 at 2188.930 kb on + strand, within ABZR87_RS10325 at 2188.966 kb on + strand, within ABZR87_RS10325 at 2188.969 kb on + strand, within ABZR87_RS10325 at 2188.969 kb on + strand, within ABZR87_RS10325 at 2188.970 kb on - strand, within ABZR87_RS10325 at 2188.970 kb on - strand, within ABZR87_RS10325 at 2189.016 kb on + strand, within ABZR87_RS10325 at 2189.016 kb on + strand, within ABZR87_RS10325 at 2189.086 kb on + strand, within ABZR87_RS10325 at 2189.086 kb on + strand, within ABZR87_RS10325 at 2189.087 kb on - strand, within ABZR87_RS10325 at 2189.087 kb on - strand, within ABZR87_RS10325 at 2189.187 kb on + strand, within ABZR87_RS10325 at 2189.187 kb on + strand, within ABZR87_RS10325 at 2189.188 kb on - strand, within ABZR87_RS10325 at 2189.188 kb on - strand, within ABZR87_RS10325 at 2189.210 kb on - strand, within ABZR87_RS10325 at 2189.210 kb on - strand, within ABZR87_RS10325 at 2189.267 kb on - strand, within ABZR87_RS10325 at 2189.267 kb on - strand, within ABZR87_RS10325 at 2189.267 kb on - strand, within ABZR87_RS10325 at 2189.705 kb on - strand, within ABZR87_RS10325 at 2189.705 kb on - strand, within ABZR87_RS10325 at 2189.731 kb on + strand, within ABZR87_RS10325 at 2189.731 kb on + strand, within ABZR87_RS10325 at 2189.731 kb on + strand, within ABZR87_RS10325 at 2189.732 kb on - strand, within ABZR87_RS10325 at 2189.732 kb on - strand, within ABZR87_RS10325 at 2189.732 kb on - strand, within ABZR87_RS10325 at 2189.945 kb on - strand, within ABZR87_RS10325 at 2189.945 kb on - strand, within ABZR87_RS10325 at 2190.010 kb on + strand, within ABZR87_RS10325 at 2190.010 kb on + strand, within ABZR87_RS10325 at 2190.010 kb on + strand, within ABZR87_RS10325 at 2190.010 kb on + strand, within ABZR87_RS10325 at 2190.011 kb on - strand, within ABZR87_RS10325 at 2190.011 kb on - strand, within ABZR87_RS10325 at 2190.011 kb on - strand, within ABZR87_RS10325 at 2190.094 kb on + strand, within ABZR87_RS10325 at 2190.178 kb on + strand, within ABZR87_RS10325 at 2190.179 kb on - strand, within ABZR87_RS10325 at 2190.501 kb on - strand at 2190.652 kb on + strand at 2190.740 kb on + strand at 2190.793 kb on + strand at 2190.889 kb on + strand, within ABZR87_RS10330 at 2190.922 kb on + strand, within ABZR87_RS10330 at 2190.922 kb on + strand, within ABZR87_RS10330 at 2190.922 kb on + strand, within ABZR87_RS10330 at 2190.923 kb on - strand, within ABZR87_RS10330 at 2190.923 kb on - strand, within ABZR87_RS10330 at 2190.964 kb on + strand, within ABZR87_RS10330 at 2191.078 kb on + strand, within ABZR87_RS10330 at 2191.078 kb on + strand, within ABZR87_RS10330 at 2191.078 kb on + strand, within ABZR87_RS10330 at 2191.079 kb on - strand, within ABZR87_RS10330 at 2191.102 kb on + strand, within ABZR87_RS10330 at 2191.102 kb on + strand, within ABZR87_RS10330 at 2191.102 kb on + strand, within ABZR87_RS10330 at 2191.102 kb on + strand, within ABZR87_RS10330 at 2191.103 kb on - strand, within ABZR87_RS10330 at 2191.103 kb on - strand, within ABZR87_RS10330 at 2191.129 kb on + strand, within ABZR87_RS10330 at 2191.130 kb on - strand, within ABZR87_RS10330 at 2191.130 kb on - strand, within ABZR87_RS10330 at 2191.130 kb on - strand, within ABZR87_RS10330 at 2191.130 kb on - strand, within ABZR87_RS10330 at 2191.147 kb on + strand, within ABZR87_RS10330 at 2191.148 kb on - strand, within ABZR87_RS10330 at 2191.255 kb on + strand, within ABZR87_RS10330 at 2191.256 kb on - strand, within ABZR87_RS10330 at 2191.256 kb on - strand, within ABZR87_RS10330 at 2191.256 kb on - strand, within ABZR87_RS10330 at 2191.256 kb on - strand, within ABZR87_RS10330 at 2191.360 kb on + strand, within ABZR87_RS10330 at 2191.361 kb on - strand, within ABZR87_RS10330 at 2191.366 kb on + strand, within ABZR87_RS10330 at 2191.532 kb on - strand, within ABZR87_RS10330 at 2191.533 kb on + strand, within ABZR87_RS10330 at 2191.533 kb on + strand, within ABZR87_RS10330 at 2191.534 kb on - strand, within ABZR87_RS10330 at 2191.534 kb on - strand, within ABZR87_RS10330 at 2191.579 kb on + strand, within ABZR87_RS10330 at 2191.579 kb on + strand, within ABZR87_RS10330 at 2191.579 kb on + strand, within ABZR87_RS10330 at 2191.580 kb on - strand, within ABZR87_RS10330 at 2191.580 kb on - strand, within ABZR87_RS10330 at 2191.627 kb on - strand, within ABZR87_RS10330 at 2191.648 kb on + strand, within ABZR87_RS10330 at 2191.648 kb on + strand, within ABZR87_RS10330 at 2191.649 kb on - strand, within ABZR87_RS10330
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-2 remove 2,187,421 - ABZR87_RS10320 0.37 +0.3 2,187,421 - ABZR87_RS10320 0.37 -0.0 2,187,492 + ABZR87_RS10320 0.43 +0.1 2,187,493 - ABZR87_RS10320 0.43 -0.8 2,187,493 - ABZR87_RS10320 0.43 +0.2 2,187,493 - ABZR87_RS10320 0.43 +0.1 2,187,693 + ABZR87_RS10320 0.58 +0.0 2,187,694 - ABZR87_RS10320 0.58 +0.3 2,187,694 - ABZR87_RS10320 0.58 -0.7 2,187,789 + ABZR87_RS10320 0.66 +0.1 2,187,913 - ABZR87_RS10320 0.75 +0.5 2,188,633 + ABZR87_RS10325 0.16 -0.5 2,188,634 - ABZR87_RS10325 0.16 -0.2 2,188,646 - ABZR87_RS10325 0.17 -3.1 2,188,663 + ABZR87_RS10325 0.17 -1.5 2,188,669 + ABZR87_RS10325 0.18 +0.7 2,188,669 + ABZR87_RS10325 0.18 -0.2 2,188,669 + ABZR87_RS10325 0.18 +0.6 2,188,670 - ABZR87_RS10325 0.18 +0.2 2,188,670 - ABZR87_RS10325 0.18 -0.8 2,188,670 - ABZR87_RS10325 0.18 -0.4 2,188,696 + ABZR87_RS10325 0.19 +0.0 2,188,696 + ABZR87_RS10325 0.19 +0.2 2,188,697 - ABZR87_RS10325 0.19 +0.5 2,188,747 + ABZR87_RS10325 0.21 +1.1 2,188,805 - ABZR87_RS10325 0.23 -0.1 2,188,805 - ABZR87_RS10325 0.23 -0.1 2,188,822 + ABZR87_RS10325 0.24 -1.5 2,188,867 + ABZR87_RS10325 0.26 -0.9 2,188,868 - ABZR87_RS10325 0.26 -0.5 2,188,914 + ABZR87_RS10325 0.28 -1.7 2,188,914 + ABZR87_RS10325 0.28 -0.6 2,188,914 + ABZR87_RS10325 0.28 -0.0 2,188,915 - ABZR87_RS10325 0.28 -0.7 2,188,918 + ABZR87_RS10325 0.28 +0.7 2,188,918 + ABZR87_RS10325 0.28 -0.1 2,188,918 + ABZR87_RS10325 0.28 -0.1 2,188,918 + ABZR87_RS10325 0.28 +0.5 2,188,919 - ABZR87_RS10325 0.28 -1.0 2,188,919 - ABZR87_RS10325 0.28 -0.6 2,188,919 - ABZR87_RS10325 0.28 +1.6 2,188,930 + ABZR87_RS10325 0.28 +0.6 2,188,966 + ABZR87_RS10325 0.30 +0.1 2,188,969 + ABZR87_RS10325 0.30 -0.9 2,188,969 + ABZR87_RS10325 0.30 +0.3 2,188,970 - ABZR87_RS10325 0.30 +0.3 2,188,970 - ABZR87_RS10325 0.30 +0.5 2,189,016 + ABZR87_RS10325 0.32 +0.1 2,189,016 + ABZR87_RS10325 0.32 -1.5 2,189,086 + ABZR87_RS10325 0.35 +0.6 2,189,086 + ABZR87_RS10325 0.35 +0.9 2,189,087 - ABZR87_RS10325 0.35 -0.5 2,189,087 - ABZR87_RS10325 0.35 +0.3 2,189,187 + ABZR87_RS10325 0.39 -0.3 2,189,187 + ABZR87_RS10325 0.39 -0.8 2,189,188 - ABZR87_RS10325 0.39 -0.5 2,189,188 - ABZR87_RS10325 0.39 -0.0 2,189,210 - ABZR87_RS10325 0.40 -0.4 2,189,210 - ABZR87_RS10325 0.40 -2.7 2,189,267 - ABZR87_RS10325 0.42 -0.1 2,189,267 - ABZR87_RS10325 0.42 +0.5 2,189,267 - ABZR87_RS10325 0.42 -0.2 2,189,705 - ABZR87_RS10325 0.61 -0.8 2,189,705 - ABZR87_RS10325 0.61 +0.1 2,189,731 + ABZR87_RS10325 0.62 +0.1 2,189,731 + ABZR87_RS10325 0.62 -0.3 2,189,731 + ABZR87_RS10325 0.62 -0.1 2,189,732 - ABZR87_RS10325 0.62 +0.0 2,189,732 - ABZR87_RS10325 0.62 -0.1 2,189,732 - ABZR87_RS10325 0.62 +0.5 2,189,945 - ABZR87_RS10325 0.71 -0.5 2,189,945 - ABZR87_RS10325 0.71 +0.8 2,190,010 + ABZR87_RS10325 0.73 +0.8 2,190,010 + ABZR87_RS10325 0.73 -0.8 2,190,010 + ABZR87_RS10325 0.73 -0.6 2,190,010 + ABZR87_RS10325 0.73 +0.0 2,190,011 - ABZR87_RS10325 0.73 -0.8 2,190,011 - ABZR87_RS10325 0.73 -0.3 2,190,011 - ABZR87_RS10325 0.73 -0.0 2,190,094 + ABZR87_RS10325 0.77 -0.7 2,190,178 + ABZR87_RS10325 0.80 -1.1 2,190,179 - ABZR87_RS10325 0.80 -0.4 2,190,501 - -2.4 2,190,652 + -0.1 2,190,740 + -0.2 2,190,793 + +0.2 2,190,889 + ABZR87_RS10330 0.12 +0.9 2,190,922 + ABZR87_RS10330 0.14 -0.1 2,190,922 + ABZR87_RS10330 0.14 +0.3 2,190,922 + ABZR87_RS10330 0.14 +0.8 2,190,923 - ABZR87_RS10330 0.14 -0.3 2,190,923 - ABZR87_RS10330 0.14 -1.0 2,190,964 + ABZR87_RS10330 0.18 +0.1 2,191,078 + ABZR87_RS10330 0.27 +0.7 2,191,078 + ABZR87_RS10330 0.27 +1.3 2,191,078 + ABZR87_RS10330 0.27 +2.6 2,191,079 - ABZR87_RS10330 0.27 -0.9 2,191,102 + ABZR87_RS10330 0.29 -0.4 2,191,102 + ABZR87_RS10330 0.29 -1.2 2,191,102 + ABZR87_RS10330 0.29 +0.9 2,191,102 + ABZR87_RS10330 0.29 -0.0 2,191,103 - ABZR87_RS10330 0.29 -0.4 2,191,103 - ABZR87_RS10330 0.29 +0.1 2,191,129 + ABZR87_RS10330 0.32 -0.1 2,191,130 - ABZR87_RS10330 0.32 +0.7 2,191,130 - ABZR87_RS10330 0.32 +0.3 2,191,130 - ABZR87_RS10330 0.32 -0.0 2,191,130 - ABZR87_RS10330 0.32 +0.6 2,191,147 + ABZR87_RS10330 0.33 -0.5 2,191,148 - ABZR87_RS10330 0.33 -1.0 2,191,255 + ABZR87_RS10330 0.42 +0.5 2,191,256 - ABZR87_RS10330 0.42 -0.2 2,191,256 - ABZR87_RS10330 0.42 +0.1 2,191,256 - ABZR87_RS10330 0.42 +2.0 2,191,256 - ABZR87_RS10330 0.42 -0.2 2,191,360 + ABZR87_RS10330 0.51 +2.4 2,191,361 - ABZR87_RS10330 0.51 +0.5 2,191,366 + ABZR87_RS10330 0.51 +0.2 2,191,532 - ABZR87_RS10330 0.65 -0.1 2,191,533 + ABZR87_RS10330 0.65 -1.0 2,191,533 + ABZR87_RS10330 0.65 +0.2 2,191,534 - ABZR87_RS10330 0.65 +0.1 2,191,534 - ABZR87_RS10330 0.65 -0.3 2,191,579 + ABZR87_RS10330 0.69 -1.3 2,191,579 + ABZR87_RS10330 0.69 +0.3 2,191,579 + ABZR87_RS10330 0.69 -1.5 2,191,580 - ABZR87_RS10330 0.69 -0.5 2,191,580 - ABZR87_RS10330 0.69 +0.5 2,191,627 - ABZR87_RS10330 0.73 -0.3 2,191,648 + ABZR87_RS10330 0.74 +1.9 2,191,648 + ABZR87_RS10330 0.74 +1.1 2,191,649 - ABZR87_RS10330 0.75 -0.8
Or see this region's nucleotide sequence