Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS22380 and ABZR87_RS22385 are separated by 30 nucleotides ABZR87_RS22385 and ABZR87_RS22390 are separated by 56 nucleotides ABZR87_RS22390 and ABZR87_RS22395 are separated by 197 nucleotides
ABZR87_RS22380: ABZR87_RS22380 - DUF4139 domain-containing protein, at 1,379,494 to 1,381,125
_RS22380
ABZR87_RS22385: ABZR87_RS22385 - cation-translocating P-type ATPase, at 1,381,156 to 1,383,537
_RS22385
ABZR87_RS22390: ABZR87_RS22390 - alpha/beta fold hydrolase, at 1,383,594 to 1,384,142
_RS22390
ABZR87_RS22395: ABZR87_RS22395 - citrate:proton symporter, at 1,384,340 to 1,385,638
_RS22395
Position (kb)
1381
1382
1383
1384 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1380.163 kb on + strand, within ABZR87_RS22380 at 1380.163 kb on + strand, within ABZR87_RS22380 at 1380.164 kb on - strand, within ABZR87_RS22380 at 1380.164 kb on - strand, within ABZR87_RS22380 at 1380.164 kb on - strand, within ABZR87_RS22380 at 1380.164 kb on - strand, within ABZR87_RS22380 at 1380.197 kb on - strand, within ABZR87_RS22380 at 1380.343 kb on + strand, within ABZR87_RS22380 at 1380.343 kb on + strand, within ABZR87_RS22380 at 1380.343 kb on + strand, within ABZR87_RS22380 at 1380.343 kb on + strand, within ABZR87_RS22380 at 1380.344 kb on - strand, within ABZR87_RS22380 at 1380.344 kb on - strand, within ABZR87_RS22380 at 1380.344 kb on - strand, within ABZR87_RS22380 at 1380.360 kb on + strand, within ABZR87_RS22380 at 1380.360 kb on + strand, within ABZR87_RS22380 at 1380.361 kb on - strand, within ABZR87_RS22380 at 1380.618 kb on + strand, within ABZR87_RS22380 at 1380.618 kb on + strand, within ABZR87_RS22380 at 1380.619 kb on - strand, within ABZR87_RS22380 at 1380.643 kb on + strand, within ABZR87_RS22380 at 1380.644 kb on - strand, within ABZR87_RS22380 at 1380.644 kb on - strand, within ABZR87_RS22380 at 1380.957 kb on + strand, within ABZR87_RS22380 at 1380.958 kb on - strand, within ABZR87_RS22380 at 1381.078 kb on + strand at 1381.078 kb on + strand at 1381.091 kb on - strand at 1381.344 kb on + strand at 1381.345 kb on - strand at 1381.345 kb on - strand at 1381.473 kb on + strand, within ABZR87_RS22385 at 1381.473 kb on + strand, within ABZR87_RS22385 at 1381.474 kb on - strand, within ABZR87_RS22385 at 1381.843 kb on + strand, within ABZR87_RS22385 at 1381.843 kb on + strand, within ABZR87_RS22385 at 1381.843 kb on + strand, within ABZR87_RS22385 at 1381.844 kb on - strand, within ABZR87_RS22385 at 1381.844 kb on - strand, within ABZR87_RS22385 at 1382.156 kb on + strand, within ABZR87_RS22385 at 1382.156 kb on + strand, within ABZR87_RS22385 at 1382.157 kb on - strand, within ABZR87_RS22385 at 1382.157 kb on - strand, within ABZR87_RS22385 at 1382.200 kb on + strand, within ABZR87_RS22385 at 1382.280 kb on - strand, within ABZR87_RS22385 at 1382.280 kb on - strand, within ABZR87_RS22385 at 1382.280 kb on - strand, within ABZR87_RS22385 at 1382.363 kb on + strand, within ABZR87_RS22385 at 1382.363 kb on + strand, within ABZR87_RS22385 at 1382.364 kb on - strand, within ABZR87_RS22385 at 1382.364 kb on - strand, within ABZR87_RS22385 at 1382.364 kb on - strand, within ABZR87_RS22385 at 1382.954 kb on - strand, within ABZR87_RS22385 at 1383.671 kb on + strand, within ABZR87_RS22390 at 1383.671 kb on + strand, within ABZR87_RS22390 at 1383.671 kb on + strand, within ABZR87_RS22390 at 1383.671 kb on + strand, within ABZR87_RS22390 at 1383.671 kb on + strand, within ABZR87_RS22390 at 1383.672 kb on - strand, within ABZR87_RS22390 at 1383.672 kb on - strand, within ABZR87_RS22390 at 1383.672 kb on - strand, within ABZR87_RS22390 at 1384.170 kb on - strand at 1384.249 kb on + strand at 1384.249 kb on + strand at 1384.280 kb on + strand at 1384.280 kb on + strand at 1384.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,380,163 + ABZR87_RS22380 0.41 -0.5 1,380,163 + ABZR87_RS22380 0.41 -0.7 1,380,164 - ABZR87_RS22380 0.41 -0.3 1,380,164 - ABZR87_RS22380 0.41 +0.2 1,380,164 - ABZR87_RS22380 0.41 +0.4 1,380,164 - ABZR87_RS22380 0.41 -0.9 1,380,197 - ABZR87_RS22380 0.43 +0.2 1,380,343 + ABZR87_RS22380 0.52 +0.5 1,380,343 + ABZR87_RS22380 0.52 +0.4 1,380,343 + ABZR87_RS22380 0.52 +0.7 1,380,343 + ABZR87_RS22380 0.52 -0.4 1,380,344 - ABZR87_RS22380 0.52 -0.0 1,380,344 - ABZR87_RS22380 0.52 +0.1 1,380,344 - ABZR87_RS22380 0.52 -0.3 1,380,360 + ABZR87_RS22380 0.53 -0.4 1,380,360 + ABZR87_RS22380 0.53 +0.2 1,380,361 - ABZR87_RS22380 0.53 +2.0 1,380,618 + ABZR87_RS22380 0.69 -2.4 1,380,618 + ABZR87_RS22380 0.69 +0.3 1,380,619 - ABZR87_RS22380 0.69 -1.5 1,380,643 + ABZR87_RS22380 0.70 -0.6 1,380,644 - ABZR87_RS22380 0.70 +0.0 1,380,644 - ABZR87_RS22380 0.70 +0.0 1,380,957 + ABZR87_RS22380 0.90 +0.1 1,380,958 - ABZR87_RS22380 0.90 -0.8 1,381,078 + +0.0 1,381,078 + -0.1 1,381,091 - +1.8 1,381,344 + -0.4 1,381,345 - -1.5 1,381,345 - -0.9 1,381,473 + ABZR87_RS22385 0.13 -0.8 1,381,473 + ABZR87_RS22385 0.13 +0.2 1,381,474 - ABZR87_RS22385 0.13 -1.9 1,381,843 + ABZR87_RS22385 0.29 -2.9 1,381,843 + ABZR87_RS22385 0.29 -1.9 1,381,843 + ABZR87_RS22385 0.29 -0.2 1,381,844 - ABZR87_RS22385 0.29 -1.9 1,381,844 - ABZR87_RS22385 0.29 -0.7 1,382,156 + ABZR87_RS22385 0.42 +0.4 1,382,156 + ABZR87_RS22385 0.42 -2.0 1,382,157 - ABZR87_RS22385 0.42 -1.9 1,382,157 - ABZR87_RS22385 0.42 -1.0 1,382,200 + ABZR87_RS22385 0.44 -0.0 1,382,280 - ABZR87_RS22385 0.47 -0.8 1,382,280 - ABZR87_RS22385 0.47 -0.9 1,382,280 - ABZR87_RS22385 0.47 -0.9 1,382,363 + ABZR87_RS22385 0.51 -0.9 1,382,363 + ABZR87_RS22385 0.51 -2.6 1,382,364 - ABZR87_RS22385 0.51 -0.6 1,382,364 - ABZR87_RS22385 0.51 +0.4 1,382,364 - ABZR87_RS22385 0.51 -2.1 1,382,954 - ABZR87_RS22385 0.75 -0.7 1,383,671 + ABZR87_RS22390 0.14 -0.6 1,383,671 + ABZR87_RS22390 0.14 -0.2 1,383,671 + ABZR87_RS22390 0.14 +1.0 1,383,671 + ABZR87_RS22390 0.14 +0.5 1,383,671 + ABZR87_RS22390 0.14 -0.3 1,383,672 - ABZR87_RS22390 0.14 +1.3 1,383,672 - ABZR87_RS22390 0.14 -1.0 1,383,672 - ABZR87_RS22390 0.14 +0.4 1,384,170 - -0.3 1,384,249 + -0.8 1,384,249 + +1.0 1,384,280 + -2.7 1,384,280 + -0.5 1,384,281 - +0.5
Or see this region's nucleotide sequence