Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS21035 and ABZR87_RS21040 overlap by 12 nucleotides ABZR87_RS21040 and ABZR87_RS21045 are separated by 15 nucleotides ABZR87_RS21045 and ABZR87_RS21050 are separated by 29 nucleotides ABZR87_RS21050 and ABZR87_RS21055 are separated by 2 nucleotides
ABZR87_RS21035: ABZR87_RS21035 - lipocalin family protein, at 1,103,914 to 1,104,546
_RS21035
ABZR87_RS21040: ABZR87_RS21040 - DUF2878 domain-containing protein, at 1,104,535 to 1,105,065
_RS21040
ABZR87_RS21045: ABZR87_RS21045 - cyclopropane-fatty-acyl-phospholipid synthase family protein, at 1,105,081 to 1,106,181
_RS21045
ABZR87_RS21050: ABZR87_RS21050 - DUF1295 domain-containing protein, at 1,106,211 to 1,106,987
_RS21050
ABZR87_RS21055: ABZR87_RS21055 - cyclopropane-fatty-acyl-phospholipid synthase family protein, at 1,106,990 to 1,108,189
_RS21055
Position (kb)
1105
1106
1107 Strain fitness (log2 ratio)
-2
-1
0
1 at 1104.131 kb on + strand, within ABZR87_RS21035 at 1104.131 kb on + strand, within ABZR87_RS21035 at 1104.131 kb on + strand, within ABZR87_RS21035 at 1104.132 kb on - strand, within ABZR87_RS21035 at 1104.132 kb on - strand, within ABZR87_RS21035 at 1104.132 kb on - strand, within ABZR87_RS21035 at 1104.132 kb on - strand, within ABZR87_RS21035 at 1104.152 kb on + strand, within ABZR87_RS21035 at 1104.153 kb on - strand, within ABZR87_RS21035 at 1104.153 kb on - strand, within ABZR87_RS21035 at 1104.266 kb on + strand, within ABZR87_RS21035 at 1104.266 kb on + strand, within ABZR87_RS21035 at 1104.266 kb on + strand, within ABZR87_RS21035 at 1104.267 kb on - strand, within ABZR87_RS21035 at 1104.267 kb on - strand, within ABZR87_RS21035 at 1104.267 kb on - strand, within ABZR87_RS21035 at 1104.267 kb on - strand, within ABZR87_RS21035 at 1104.267 kb on - strand, within ABZR87_RS21035 at 1104.312 kb on - strand, within ABZR87_RS21035 at 1104.342 kb on - strand, within ABZR87_RS21035 at 1104.362 kb on + strand, within ABZR87_RS21035 at 1104.362 kb on + strand, within ABZR87_RS21035 at 1104.362 kb on + strand, within ABZR87_RS21035 at 1104.362 kb on + strand, within ABZR87_RS21035 at 1104.362 kb on + strand, within ABZR87_RS21035 at 1104.362 kb on + strand, within ABZR87_RS21035 at 1104.362 kb on + strand, within ABZR87_RS21035 at 1104.363 kb on - strand, within ABZR87_RS21035 at 1104.363 kb on - strand, within ABZR87_RS21035 at 1104.377 kb on + strand, within ABZR87_RS21035 at 1104.378 kb on - strand, within ABZR87_RS21035 at 1104.559 kb on + strand at 1104.560 kb on - strand at 1104.750 kb on - strand, within ABZR87_RS21040 at 1105.004 kb on + strand, within ABZR87_RS21040 at 1105.068 kb on - strand at 1105.101 kb on - strand at 1105.101 kb on - strand at 1105.134 kb on - strand at 1105.160 kb on + strand at 1105.160 kb on + strand at 1105.160 kb on + strand at 1105.208 kb on + strand, within ABZR87_RS21045 at 1105.286 kb on + strand, within ABZR87_RS21045 at 1105.385 kb on + strand, within ABZR87_RS21045 at 1105.386 kb on - strand, within ABZR87_RS21045 at 1105.514 kb on + strand, within ABZR87_RS21045 at 1105.514 kb on + strand, within ABZR87_RS21045 at 1105.515 kb on - strand, within ABZR87_RS21045 at 1105.802 kb on + strand, within ABZR87_RS21045 at 1105.857 kb on - strand, within ABZR87_RS21045 at 1105.871 kb on + strand, within ABZR87_RS21045 at 1105.922 kb on + strand, within ABZR87_RS21045 at 1106.200 kb on + strand at 1106.201 kb on - strand at 1106.201 kb on - strand at 1106.201 kb on - strand at 1106.209 kb on + strand at 1106.238 kb on + strand at 1106.238 kb on + strand at 1106.239 kb on - strand at 1106.257 kb on + strand at 1106.257 kb on + strand at 1106.257 kb on + strand at 1106.266 kb on + strand at 1106.267 kb on - strand at 1106.267 kb on - strand at 1106.288 kb on + strand at 1106.288 kb on + strand at 1106.398 kb on + strand, within ABZR87_RS21050 at 1106.398 kb on + strand, within ABZR87_RS21050 at 1106.399 kb on - strand, within ABZR87_RS21050 at 1106.399 kb on - strand, within ABZR87_RS21050 at 1106.428 kb on + strand, within ABZR87_RS21050
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,104,131 + ABZR87_RS21035 0.34 +0.6 1,104,131 + ABZR87_RS21035 0.34 +0.4 1,104,131 + ABZR87_RS21035 0.34 -0.4 1,104,132 - ABZR87_RS21035 0.34 +1.2 1,104,132 - ABZR87_RS21035 0.34 +1.1 1,104,132 - ABZR87_RS21035 0.34 +0.7 1,104,132 - ABZR87_RS21035 0.34 +0.7 1,104,152 + ABZR87_RS21035 0.38 +0.1 1,104,153 - ABZR87_RS21035 0.38 +0.9 1,104,153 - ABZR87_RS21035 0.38 -2.1 1,104,266 + ABZR87_RS21035 0.56 -0.7 1,104,266 + ABZR87_RS21035 0.56 -0.7 1,104,266 + ABZR87_RS21035 0.56 +1.0 1,104,267 - ABZR87_RS21035 0.56 -0.5 1,104,267 - ABZR87_RS21035 0.56 -0.3 1,104,267 - ABZR87_RS21035 0.56 -0.8 1,104,267 - ABZR87_RS21035 0.56 -0.0 1,104,267 - ABZR87_RS21035 0.56 -0.8 1,104,312 - ABZR87_RS21035 0.63 -1.0 1,104,342 - ABZR87_RS21035 0.68 -0.7 1,104,362 + ABZR87_RS21035 0.71 -0.5 1,104,362 + ABZR87_RS21035 0.71 -0.4 1,104,362 + ABZR87_RS21035 0.71 +1.0 1,104,362 + ABZR87_RS21035 0.71 -0.6 1,104,362 + ABZR87_RS21035 0.71 +0.1 1,104,362 + ABZR87_RS21035 0.71 -0.2 1,104,362 + ABZR87_RS21035 0.71 +0.6 1,104,363 - ABZR87_RS21035 0.71 -1.2 1,104,363 - ABZR87_RS21035 0.71 -0.6 1,104,377 + ABZR87_RS21035 0.73 -0.7 1,104,378 - ABZR87_RS21035 0.73 +1.0 1,104,559 + -0.7 1,104,560 - +1.4 1,104,750 - ABZR87_RS21040 0.40 +0.3 1,105,004 + ABZR87_RS21040 0.88 +0.8 1,105,068 - -1.0 1,105,101 - -1.0 1,105,101 - -0.5 1,105,134 - +1.0 1,105,160 + -0.1 1,105,160 + +0.4 1,105,160 + -0.2 1,105,208 + ABZR87_RS21045 0.12 +0.2 1,105,286 + ABZR87_RS21045 0.19 -0.2 1,105,385 + ABZR87_RS21045 0.28 -0.6 1,105,386 - ABZR87_RS21045 0.28 -0.2 1,105,514 + ABZR87_RS21045 0.39 +1.0 1,105,514 + ABZR87_RS21045 0.39 -1.5 1,105,515 - ABZR87_RS21045 0.39 -0.5 1,105,802 + ABZR87_RS21045 0.65 -1.5 1,105,857 - ABZR87_RS21045 0.70 -1.3 1,105,871 + ABZR87_RS21045 0.72 -0.2 1,105,922 + ABZR87_RS21045 0.76 -0.2 1,106,200 + -1.2 1,106,201 - +0.4 1,106,201 - +0.8 1,106,201 - -0.4 1,106,209 + +1.0 1,106,238 + -0.3 1,106,238 + +0.4 1,106,239 - +0.4 1,106,257 + -0.5 1,106,257 + -0.8 1,106,257 + -2.3 1,106,266 + -0.2 1,106,267 - -0.3 1,106,267 - +1.1 1,106,288 + -0.2 1,106,288 + -0.3 1,106,398 + ABZR87_RS21050 0.24 -0.4 1,106,398 + ABZR87_RS21050 0.24 +1.0 1,106,399 - ABZR87_RS21050 0.24 -0.2 1,106,399 - ABZR87_RS21050 0.24 +0.7 1,106,428 + ABZR87_RS21050 0.28 +0.2
Or see this region's nucleotide sequence