Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS19535 and ABZR87_RS19540 are separated by 9 nucleotides ABZR87_RS19540 and ABZR87_RS19545 overlap by 4 nucleotides
ABZR87_RS19535: ABZR87_RS19535 - type II and III secretion system protein family protein, at 754,470 to 755,834
_RS19535
ABZR87_RS19540: ABZR87_RS19540 - CpaE family protein, at 755,844 to 757,187
_RS19540
ABZR87_RS19545: ABZR87_RS19545 - CpaF family protein, at 757,184 to 758,539
_RS19545
Position (kb)
755
756
757
758 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.867 kb on + strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.868 kb on - strand, within ABZR87_RS19535 at 754.869 kb on + strand, within ABZR87_RS19535 at 755.343 kb on + strand, within ABZR87_RS19535 at 755.343 kb on + strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.344 kb on - strand, within ABZR87_RS19535 at 755.501 kb on + strand, within ABZR87_RS19535 at 755.567 kb on - strand, within ABZR87_RS19535 at 755.637 kb on + strand, within ABZR87_RS19535 at 755.637 kb on + strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.638 kb on - strand, within ABZR87_RS19535 at 755.672 kb on + strand, within ABZR87_RS19535 at 755.673 kb on - strand, within ABZR87_RS19535 at 755.832 kb on + strand at 755.832 kb on + strand at 755.833 kb on - strand at 755.833 kb on - strand at 755.833 kb on - strand at 755.850 kb on + strand at 755.850 kb on + strand at 755.850 kb on + strand at 755.850 kb on + strand at 755.851 kb on - strand at 755.851 kb on - strand at 755.851 kb on - strand at 755.851 kb on - strand at 756.058 kb on + strand, within ABZR87_RS19540 at 756.058 kb on + strand, within ABZR87_RS19540 at 756.058 kb on + strand, within ABZR87_RS19540 at 756.460 kb on - strand, within ABZR87_RS19540 at 756.460 kb on - strand, within ABZR87_RS19540 at 756.854 kb on + strand, within ABZR87_RS19540 at 756.854 kb on + strand, within ABZR87_RS19540 at 756.854 kb on + strand, within ABZR87_RS19540 at 756.996 kb on + strand, within ABZR87_RS19540 at 756.996 kb on + strand, within ABZR87_RS19540 at 756.997 kb on - strand, within ABZR87_RS19540 at 756.997 kb on - strand, within ABZR87_RS19540 at 757.378 kb on + strand, within ABZR87_RS19545 at 757.378 kb on + strand, within ABZR87_RS19545 at 757.379 kb on - strand, within ABZR87_RS19545 at 757.529 kb on + strand, within ABZR87_RS19545 at 757.529 kb on + strand, within ABZR87_RS19545 at 757.529 kb on + strand, within ABZR87_RS19545 at 757.529 kb on + strand, within ABZR87_RS19545 at 757.530 kb on - strand, within ABZR87_RS19545 at 757.530 kb on - strand, within ABZR87_RS19545 at 757.530 kb on - strand, within ABZR87_RS19545 at 757.530 kb on - strand, within ABZR87_RS19545 at 757.530 kb on - strand, within ABZR87_RS19545 at 757.901 kb on + strand, within ABZR87_RS19545 at 757.902 kb on - strand, within ABZR87_RS19545 at 757.902 kb on - strand, within ABZR87_RS19545 at 758.129 kb on - strand, within ABZR87_RS19545
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 754,867 + ABZR87_RS19535 0.29 -1.4 754,867 + ABZR87_RS19535 0.29 -1.1 754,867 + ABZR87_RS19535 0.29 -1.3 754,867 + ABZR87_RS19535 0.29 +0.5 754,868 - ABZR87_RS19535 0.29 -1.2 754,868 - ABZR87_RS19535 0.29 -0.2 754,868 - ABZR87_RS19535 0.29 -1.8 754,868 - ABZR87_RS19535 0.29 -2.1 754,868 - ABZR87_RS19535 0.29 -1.7 754,868 - ABZR87_RS19535 0.29 +0.0 754,868 - ABZR87_RS19535 0.29 -1.1 754,868 - ABZR87_RS19535 0.29 -3.4 754,868 - ABZR87_RS19535 0.29 +0.0 754,868 - ABZR87_RS19535 0.29 -0.4 754,868 - ABZR87_RS19535 0.29 -1.0 754,868 - ABZR87_RS19535 0.29 -1.1 754,869 + ABZR87_RS19535 0.29 -0.3 755,343 + ABZR87_RS19535 0.64 -0.6 755,343 + ABZR87_RS19535 0.64 -0.9 755,344 - ABZR87_RS19535 0.64 -0.1 755,344 - ABZR87_RS19535 0.64 -1.1 755,344 - ABZR87_RS19535 0.64 -0.1 755,344 - ABZR87_RS19535 0.64 +0.3 755,501 + ABZR87_RS19535 0.76 +0.4 755,567 - ABZR87_RS19535 0.80 +0.2 755,637 + ABZR87_RS19535 0.85 -1.0 755,637 + ABZR87_RS19535 0.85 +0.1 755,638 - ABZR87_RS19535 0.86 -0.4 755,638 - ABZR87_RS19535 0.86 -0.7 755,638 - ABZR87_RS19535 0.86 -1.2 755,638 - ABZR87_RS19535 0.86 -1.1 755,638 - ABZR87_RS19535 0.86 -1.5 755,672 + ABZR87_RS19535 0.88 -0.9 755,673 - ABZR87_RS19535 0.88 +0.2 755,832 + -0.3 755,832 + +0.4 755,833 - -2.2 755,833 - -0.7 755,833 - -1.2 755,850 + -1.4 755,850 + -1.0 755,850 + +1.2 755,850 + -0.2 755,851 - -0.5 755,851 - +0.4 755,851 - +0.6 755,851 - -0.5 756,058 + ABZR87_RS19540 0.16 -1.1 756,058 + ABZR87_RS19540 0.16 -0.9 756,058 + ABZR87_RS19540 0.16 -0.4 756,460 - ABZR87_RS19540 0.46 -0.6 756,460 - ABZR87_RS19540 0.46 -0.4 756,854 + ABZR87_RS19540 0.75 -2.0 756,854 + ABZR87_RS19540 0.75 -0.1 756,854 + ABZR87_RS19540 0.75 -0.6 756,996 + ABZR87_RS19540 0.86 +0.3 756,996 + ABZR87_RS19540 0.86 -1.5 756,997 - ABZR87_RS19540 0.86 -0.6 756,997 - ABZR87_RS19540 0.86 -0.1 757,378 + ABZR87_RS19545 0.14 +0.2 757,378 + ABZR87_RS19545 0.14 +0.2 757,379 - ABZR87_RS19545 0.14 -1.5 757,529 + ABZR87_RS19545 0.25 -0.8 757,529 + ABZR87_RS19545 0.25 -1.4 757,529 + ABZR87_RS19545 0.25 -0.4 757,529 + ABZR87_RS19545 0.25 +0.2 757,530 - ABZR87_RS19545 0.26 +0.4 757,530 - ABZR87_RS19545 0.26 +0.6 757,530 - ABZR87_RS19545 0.26 -0.7 757,530 - ABZR87_RS19545 0.26 -0.9 757,530 - ABZR87_RS19545 0.26 -1.0 757,901 + ABZR87_RS19545 0.53 +1.4 757,902 - ABZR87_RS19545 0.53 -1.3 757,902 - ABZR87_RS19545 0.53 -1.1 758,129 - ABZR87_RS19545 0.70 +1.3
Or see this region's nucleotide sequence