Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS17930 and ABZR87_RS17935 are separated by 76 nucleotides ABZR87_RS17935 and ABZR87_RS17940 are separated by 71 nucleotides ABZR87_RS17940 and ABZR87_RS17945 are separated by 107 nucleotides
ABZR87_RS17930: ABZR87_RS17930 - flagellar transcriptional regulator FlhD, at 378,977 to 379,294
_RS17930
ABZR87_RS17935: ABZR87_RS17935 - flagellar transcriptional regulator FlhC, at 379,371 to 379,931
_RS17935
ABZR87_RS17940: ABZR87_RS17940 - hypothetical protein, at 380,003 to 380,374
_RS17940
ABZR87_RS17945: ABZR87_RS17945 - flagellar motor stator protein MotA, at 380,482 to 381,342
_RS17945
Position (kb) 379
380 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 378.387 kb on + strand at 378.387 kb on + strand at 378.387 kb on + strand at 378.388 kb on - strand at 378.434 kb on - strand at 378.444 kb on + strand at 378.444 kb on + strand at 378.444 kb on + strand at 378.445 kb on - strand at 378.445 kb on - strand at 378.466 kb on + strand at 378.559 kb on - strand at 378.585 kb on - strand at 378.585 kb on - strand at 378.585 kb on - strand at 378.585 kb on - strand at 378.659 kb on + strand at 378.660 kb on - strand at 378.660 kb on - strand at 378.660 kb on - strand at 378.674 kb on - strand at 378.719 kb on + strand at 378.720 kb on - strand at 378.738 kb on - strand at 378.738 kb on - strand at 378.738 kb on - strand at 378.738 kb on - strand at 378.738 kb on - strand at 378.741 kb on + strand at 378.796 kb on + strand at 378.808 kb on + strand at 378.808 kb on + strand at 378.809 kb on - strand at 378.809 kb on - strand at 378.809 kb on - strand at 378.809 kb on - strand at 378.809 kb on - strand at 378.892 kb on + strand at 378.893 kb on - strand at 378.907 kb on - strand at 378.907 kb on - strand at 378.949 kb on + strand at 378.949 kb on + strand at 378.949 kb on + strand at 378.950 kb on - strand at 378.950 kb on - strand at 378.955 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.974 kb on + strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 378.975 kb on - strand at 379.025 kb on + strand, within ABZR87_RS17930 at 379.025 kb on + strand, within ABZR87_RS17930 at 379.025 kb on + strand, within ABZR87_RS17930 at 379.025 kb on + strand, within ABZR87_RS17930 at 379.025 kb on + strand, within ABZR87_RS17930 at 379.026 kb on - strand, within ABZR87_RS17930 at 379.026 kb on - strand, within ABZR87_RS17930 at 379.026 kb on - strand, within ABZR87_RS17930 at 379.026 kb on - strand, within ABZR87_RS17930 at 379.346 kb on + strand at 379.346 kb on + strand at 379.346 kb on + strand at 379.346 kb on + strand at 379.346 kb on + strand at 379.347 kb on - strand at 379.347 kb on - strand at 379.347 kb on - strand at 379.500 kb on + strand, within ABZR87_RS17935 at 379.500 kb on + strand, within ABZR87_RS17935 at 379.500 kb on + strand, within ABZR87_RS17935 at 379.501 kb on - strand, within ABZR87_RS17935 at 379.501 kb on - strand, within ABZR87_RS17935 at 379.608 kb on + strand, within ABZR87_RS17935 at 379.608 kb on + strand, within ABZR87_RS17935 at 379.609 kb on - strand, within ABZR87_RS17935 at 379.665 kb on + strand, within ABZR87_RS17935 at 379.665 kb on + strand, within ABZR87_RS17935 at 379.675 kb on - strand, within ABZR87_RS17935 at 379.932 kb on + strand at 379.933 kb on - strand at 379.965 kb on + strand at 379.965 kb on + strand at 379.966 kb on - strand at 379.966 kb on - strand at 379.966 kb on - strand at 379.966 kb on - strand at 379.974 kb on - strand at 380.025 kb on + strand at 380.026 kb on - strand at 380.026 kb on - strand at 380.031 kb on + strand at 380.037 kb on + strand at 380.043 kb on - strand, within ABZR87_RS17940 at 380.047 kb on - strand, within ABZR87_RS17940 at 380.062 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.082 kb on + strand, within ABZR87_RS17940 at 380.083 kb on - strand, within ABZR87_RS17940 at 380.083 kb on - strand, within ABZR87_RS17940 at 380.083 kb on - strand, within ABZR87_RS17940 at 380.083 kb on - strand, within ABZR87_RS17940 at 380.083 kb on - strand, within ABZR87_RS17940 at 380.083 kb on - strand, within ABZR87_RS17940 at 380.083 kb on - strand, within ABZR87_RS17940 at 380.083 kb on - strand, within ABZR87_RS17940 at 380.083 kb on - strand, within ABZR87_RS17940 at 380.088 kb on + strand, within ABZR87_RS17940 at 380.088 kb on + strand, within ABZR87_RS17940 at 380.088 kb on + strand, within ABZR87_RS17940 at 380.088 kb on + strand, within ABZR87_RS17940 at 380.088 kb on + strand, within ABZR87_RS17940 at 380.088 kb on + strand, within ABZR87_RS17940 at 380.088 kb on + strand, within ABZR87_RS17940 at 380.088 kb on + strand, within ABZR87_RS17940 at 380.089 kb on - strand, within ABZR87_RS17940 at 380.089 kb on - strand, within ABZR87_RS17940 at 380.089 kb on - strand, within ABZR87_RS17940 at 380.089 kb on - strand, within ABZR87_RS17940 at 380.089 kb on - strand, within ABZR87_RS17940 at 380.089 kb on - strand, within ABZR87_RS17940 at 380.089 kb on - strand, within ABZR87_RS17940 at 380.212 kb on + strand, within ABZR87_RS17940 at 380.212 kb on + strand, within ABZR87_RS17940 at 380.213 kb on - strand, within ABZR87_RS17940 at 380.213 kb on - strand, within ABZR87_RS17940 at 380.213 kb on - strand, within ABZR87_RS17940 at 380.371 kb on - strand at 380.433 kb on + strand at 380.445 kb on + strand at 380.445 kb on + strand at 380.445 kb on + strand at 380.446 kb on - strand at 380.446 kb on - strand at 380.446 kb on - strand at 380.486 kb on + strand at 380.486 kb on + strand at 380.487 kb on - strand at 380.500 kb on + strand at 380.501 kb on - strand at 380.533 kb on + strand at 380.533 kb on + strand at 380.533 kb on + strand at 380.533 kb on + strand at 380.533 kb on + strand at 380.533 kb on + strand at 380.534 kb on - strand at 380.534 kb on - strand at 380.704 kb on + strand, within ABZR87_RS17945 at 380.704 kb on + strand, within ABZR87_RS17945 at 380.704 kb on + strand, within ABZR87_RS17945 at 380.704 kb on + strand, within ABZR87_RS17945 at 380.704 kb on + strand, within ABZR87_RS17945 at 380.704 kb on + strand, within ABZR87_RS17945 at 380.705 kb on - strand, within ABZR87_RS17945 at 380.705 kb on - strand, within ABZR87_RS17945 at 380.705 kb on - strand, within ABZR87_RS17945 at 380.705 kb on - strand, within ABZR87_RS17945 at 380.705 kb on - strand, within ABZR87_RS17945 at 380.705 kb on - strand, within ABZR87_RS17945 at 380.705 kb on - strand, within ABZR87_RS17945 at 380.706 kb on + strand, within ABZR87_RS17945 at 380.706 kb on + strand, within ABZR87_RS17945 at 380.707 kb on - strand, within ABZR87_RS17945 at 380.707 kb on - strand, within ABZR87_RS17945 at 380.707 kb on - strand, within ABZR87_RS17945 at 380.707 kb on - strand, within ABZR87_RS17945 at 380.728 kb on + strand, within ABZR87_RS17945 at 380.728 kb on + strand, within ABZR87_RS17945 at 380.728 kb on + strand, within ABZR87_RS17945 at 380.728 kb on + strand, within ABZR87_RS17945 at 380.728 kb on + strand, within ABZR87_RS17945 at 380.729 kb on - strand, within ABZR87_RS17945 at 380.729 kb on - strand, within ABZR87_RS17945 at 380.818 kb on + strand, within ABZR87_RS17945 at 380.818 kb on + strand, within ABZR87_RS17945 at 380.818 kb on + strand, within ABZR87_RS17945 at 380.818 kb on + strand, within ABZR87_RS17945 at 380.819 kb on - strand, within ABZR87_RS17945 at 380.819 kb on - strand, within ABZR87_RS17945 at 380.867 kb on - strand, within ABZR87_RS17945
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 378,387 + +1.9 378,387 + +1.0 378,387 + +1.2 378,388 - +1.5 378,434 - +1.8 378,444 + +1.8 378,444 + +1.6 378,444 + +2.2 378,445 - +1.9 378,445 - +2.2 378,466 + +2.0 378,559 - +1.2 378,585 - +0.9 378,585 - -0.0 378,585 - +2.0 378,585 - +1.3 378,659 + +1.0 378,660 - +2.0 378,660 - +1.4 378,660 - +1.0 378,674 - +1.9 378,719 + -0.2 378,720 - +0.0 378,738 - +0.3 378,738 - -0.2 378,738 - +0.5 378,738 - -0.5 378,738 - -1.9 378,741 + -0.5 378,796 + -0.8 378,808 + +0.5 378,808 + -0.5 378,809 - +1.0 378,809 - -0.5 378,809 - -0.3 378,809 - +0.1 378,809 - -0.4 378,892 + -1.6 378,893 - +0.3 378,907 - -0.3 378,907 - +0.1 378,949 + +1.0 378,949 + +1.1 378,949 + +0.9 378,950 - -1.2 378,950 - -2.2 378,955 + -0.5 378,974 + -0.4 378,974 + -0.5 378,974 + -1.4 378,974 + -0.8 378,974 + -0.5 378,974 + -1.4 378,974 + -1.3 378,974 + -2.3 378,974 + +1.8 378,974 + -0.3 378,974 + +0.3 378,974 + +0.3 378,974 + -0.7 378,974 + -0.4 378,974 + +0.4 378,975 - -1.3 378,975 - -0.8 378,975 - +0.2 378,975 - -0.2 378,975 - -1.2 378,975 - -0.5 378,975 - +0.2 378,975 - -0.7 378,975 - -0.4 378,975 - -0.3 378,975 - +0.1 378,975 - +0.2 378,975 - -1.1 378,975 - -0.5 378,975 - -1.6 378,975 - -1.3 378,975 - +0.3 378,975 - -0.8 379,025 + ABZR87_RS17930 0.15 +2.2 379,025 + ABZR87_RS17930 0.15 -0.1 379,025 + ABZR87_RS17930 0.15 -0.4 379,025 + ABZR87_RS17930 0.15 -0.4 379,025 + ABZR87_RS17930 0.15 -0.3 379,026 - ABZR87_RS17930 0.15 -0.3 379,026 - ABZR87_RS17930 0.15 -1.2 379,026 - ABZR87_RS17930 0.15 +0.1 379,026 - ABZR87_RS17930 0.15 -1.1 379,346 + -0.6 379,346 + -0.4 379,346 + -0.5 379,346 + -2.3 379,346 + -0.4 379,347 - -0.7 379,347 - +1.4 379,347 - -0.4 379,500 + ABZR87_RS17935 0.23 -1.5 379,500 + ABZR87_RS17935 0.23 -0.3 379,500 + ABZR87_RS17935 0.23 -0.8 379,501 - ABZR87_RS17935 0.23 -0.6 379,501 - ABZR87_RS17935 0.23 -0.5 379,608 + ABZR87_RS17935 0.42 -0.5 379,608 + ABZR87_RS17935 0.42 -1.1 379,609 - ABZR87_RS17935 0.42 -1.6 379,665 + ABZR87_RS17935 0.52 -0.6 379,665 + ABZR87_RS17935 0.52 -1.2 379,675 - ABZR87_RS17935 0.54 -0.0 379,932 + -0.8 379,933 - -0.8 379,965 + -0.2 379,965 + -1.2 379,966 - -1.3 379,966 - -0.7 379,966 - -0.2 379,966 - -0.1 379,974 - -0.1 380,025 + -1.2 380,026 - -0.3 380,026 - -0.5 380,031 + +0.4 380,037 + -0.1 380,043 - ABZR87_RS17940 0.11 -0.2 380,047 - ABZR87_RS17940 0.12 +0.5 380,062 + ABZR87_RS17940 0.16 -1.5 380,082 + ABZR87_RS17940 0.21 -0.1 380,082 + ABZR87_RS17940 0.21 +2.0 380,082 + ABZR87_RS17940 0.21 +0.9 380,082 + ABZR87_RS17940 0.21 -0.5 380,082 + ABZR87_RS17940 0.21 -0.3 380,082 + ABZR87_RS17940 0.21 +0.4 380,082 + ABZR87_RS17940 0.21 +0.2 380,082 + ABZR87_RS17940 0.21 -0.6 380,082 + ABZR87_RS17940 0.21 -0.3 380,082 + ABZR87_RS17940 0.21 -0.4 380,082 + ABZR87_RS17940 0.21 -0.2 380,082 + ABZR87_RS17940 0.21 -0.1 380,082 + ABZR87_RS17940 0.21 -0.3 380,082 + ABZR87_RS17940 0.21 +0.6 380,082 + ABZR87_RS17940 0.21 -0.3 380,083 - ABZR87_RS17940 0.22 -0.6 380,083 - ABZR87_RS17940 0.22 -1.1 380,083 - ABZR87_RS17940 0.22 -1.0 380,083 - ABZR87_RS17940 0.22 -1.6 380,083 - ABZR87_RS17940 0.22 -0.1 380,083 - ABZR87_RS17940 0.22 -0.2 380,083 - ABZR87_RS17940 0.22 +1.1 380,083 - ABZR87_RS17940 0.22 -0.1 380,083 - ABZR87_RS17940 0.22 +0.3 380,088 + ABZR87_RS17940 0.23 +0.2 380,088 + ABZR87_RS17940 0.23 +1.0 380,088 + ABZR87_RS17940 0.23 +0.1 380,088 + ABZR87_RS17940 0.23 -0.4 380,088 + ABZR87_RS17940 0.23 -0.5 380,088 + ABZR87_RS17940 0.23 +0.0 380,088 + ABZR87_RS17940 0.23 +1.3 380,088 + ABZR87_RS17940 0.23 -0.5 380,089 - ABZR87_RS17940 0.23 -0.6 380,089 - ABZR87_RS17940 0.23 -0.1 380,089 - ABZR87_RS17940 0.23 +1.1 380,089 - ABZR87_RS17940 0.23 -0.0 380,089 - ABZR87_RS17940 0.23 -0.7 380,089 - ABZR87_RS17940 0.23 -0.6 380,089 - ABZR87_RS17940 0.23 -0.3 380,212 + ABZR87_RS17940 0.56 -1.5 380,212 + ABZR87_RS17940 0.56 -0.3 380,213 - ABZR87_RS17940 0.56 -0.4 380,213 - ABZR87_RS17940 0.56 -0.1 380,213 - ABZR87_RS17940 0.56 -0.4 380,371 - +0.1 380,433 + -0.0 380,445 + -1.2 380,445 + +0.1 380,445 + +0.1 380,446 - -1.2 380,446 - -0.0 380,446 - -0.5 380,486 + -2.2 380,486 + -0.7 380,487 - -0.2 380,500 + -0.5 380,501 - +0.2 380,533 + -1.4 380,533 + -0.8 380,533 + +0.7 380,533 + -0.0 380,533 + -0.6 380,533 + -1.4 380,534 - -0.8 380,534 - -1.1 380,704 + ABZR87_RS17945 0.26 -1.5 380,704 + ABZR87_RS17945 0.26 -1.0 380,704 + ABZR87_RS17945 0.26 -0.7 380,704 + ABZR87_RS17945 0.26 -0.7 380,704 + ABZR87_RS17945 0.26 +1.4 380,704 + ABZR87_RS17945 0.26 -0.1 380,705 - ABZR87_RS17945 0.26 -0.3 380,705 - ABZR87_RS17945 0.26 -1.7 380,705 - ABZR87_RS17945 0.26 +0.8 380,705 - ABZR87_RS17945 0.26 -0.3 380,705 - ABZR87_RS17945 0.26 -2.9 380,705 - ABZR87_RS17945 0.26 -1.5 380,705 - ABZR87_RS17945 0.26 -1.4 380,706 + ABZR87_RS17945 0.26 -1.2 380,706 + ABZR87_RS17945 0.26 +0.1 380,707 - ABZR87_RS17945 0.26 -0.8 380,707 - ABZR87_RS17945 0.26 +0.2 380,707 - ABZR87_RS17945 0.26 +0.2 380,707 - ABZR87_RS17945 0.26 -0.4 380,728 + ABZR87_RS17945 0.29 -0.2 380,728 + ABZR87_RS17945 0.29 -0.4 380,728 + ABZR87_RS17945 0.29 -0.8 380,728 + ABZR87_RS17945 0.29 -0.3 380,728 + ABZR87_RS17945 0.29 +0.1 380,729 - ABZR87_RS17945 0.29 +0.7 380,729 - ABZR87_RS17945 0.29 +0.0 380,818 + ABZR87_RS17945 0.39 -0.2 380,818 + ABZR87_RS17945 0.39 -0.4 380,818 + ABZR87_RS17945 0.39 +0.1 380,818 + ABZR87_RS17945 0.39 +0.9 380,819 - ABZR87_RS17945 0.39 -0.9 380,819 - ABZR87_RS17945 0.39 +0.3 380,867 - ABZR87_RS17945 0.45 -1.2
Or see this region's nucleotide sequence