Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS16950 and ABZR87_RS16955 are separated by 209 nucleotides ABZR87_RS16955 and ABZR87_RS16960 are separated by 8 nucleotides ABZR87_RS16960 and ABZR87_RS16965 are separated by 42 nucleotides ABZR87_RS16965 and ABZR87_RS16970 overlap by 4 nucleotides
ABZR87_RS16950: ABZR87_RS16950 - cytochrome c biogenesis protein DipZ, at 156,578 to 158,347
_RS16950
ABZR87_RS16955: ABZR87_RS16955 - response regulator, at 158,557 to 159,300
_RS16955
ABZR87_RS16960: ABZR87_RS16960 - HAMP domain-containing sensor histidine kinase, at 159,309 to 160,625
_RS16960
ABZR87_RS16965: ABZR87_RS16965 - DUF72 domain-containing protein, at 160,668 to 161,543
_RS16965
ABZR87_RS16970: ABZR87_RS16970 - SIR2 family protein, at 161,540 to 162,463
_RS16970
Position (kb) 159
160
161 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 158.325 kb on + strand at 158.325 kb on + strand at 158.333 kb on + strand at 158.334 kb on - strand at 158.334 kb on - strand at 158.413 kb on + strand at 158.413 kb on + strand at 158.414 kb on - strand at 158.414 kb on - strand at 158.414 kb on - strand at 158.414 kb on - strand at 158.414 kb on - strand at 158.414 kb on - strand at 158.435 kb on + strand at 158.436 kb on - strand at 158.445 kb on + strand at 158.445 kb on + strand at 158.446 kb on - strand at 158.446 kb on - strand at 158.446 kb on - strand at 158.446 kb on - strand at 158.474 kb on + strand at 158.474 kb on + strand at 158.475 kb on - strand at 158.626 kb on + strand at 158.627 kb on - strand at 158.627 kb on - strand at 158.627 kb on - strand at 158.627 kb on - strand at 158.798 kb on + strand, within ABZR87_RS16955 at 158.798 kb on + strand, within ABZR87_RS16955 at 158.799 kb on - strand, within ABZR87_RS16955 at 158.918 kb on + strand, within ABZR87_RS16955 at 158.918 kb on + strand, within ABZR87_RS16955 at 158.919 kb on - strand, within ABZR87_RS16955 at 159.065 kb on - strand, within ABZR87_RS16955 at 159.229 kb on + strand at 159.229 kb on + strand at 159.230 kb on - strand at 159.256 kb on + strand at 159.256 kb on + strand at 159.256 kb on + strand at 159.256 kb on + strand at 159.256 kb on + strand at 159.256 kb on + strand at 159.256 kb on + strand at 159.256 kb on + strand at 159.257 kb on - strand at 159.257 kb on - strand at 159.257 kb on - strand at 159.257 kb on - strand at 159.257 kb on - strand at 159.390 kb on + strand at 159.391 kb on - strand at 159.426 kb on + strand at 159.426 kb on + strand at 159.427 kb on - strand at 159.552 kb on + strand, within ABZR87_RS16960 at 159.553 kb on - strand, within ABZR87_RS16960 at 159.553 kb on - strand, within ABZR87_RS16960 at 159.553 kb on - strand, within ABZR87_RS16960 at 159.558 kb on + strand, within ABZR87_RS16960 at 159.558 kb on + strand, within ABZR87_RS16960 at 159.558 kb on + strand, within ABZR87_RS16960 at 159.559 kb on - strand, within ABZR87_RS16960 at 159.559 kb on - strand, within ABZR87_RS16960 at 159.559 kb on - strand, within ABZR87_RS16960 at 159.559 kb on - strand, within ABZR87_RS16960 at 159.781 kb on - strand, within ABZR87_RS16960 at 159.781 kb on - strand, within ABZR87_RS16960 at 160.149 kb on + strand, within ABZR87_RS16960 at 160.150 kb on - strand, within ABZR87_RS16960 at 160.224 kb on + strand, within ABZR87_RS16960 at 160.224 kb on + strand, within ABZR87_RS16960 at 160.224 kb on + strand, within ABZR87_RS16960 at 160.225 kb on - strand, within ABZR87_RS16960 at 160.731 kb on - strand at 160.731 kb on - strand at 160.744 kb on + strand at 160.744 kb on + strand at 160.744 kb on + strand at 160.744 kb on + strand at 160.750 kb on + strand at 160.750 kb on + strand at 160.750 kb on + strand at 160.750 kb on + strand at 160.750 kb on + strand at 160.750 kb on + strand at 160.750 kb on + strand at 160.750 kb on + strand at 160.750 kb on + strand at 160.750 kb on + strand at 160.751 kb on - strand at 160.751 kb on - strand at 160.751 kb on - strand at 160.751 kb on - strand at 160.751 kb on - strand at 160.751 kb on - strand at 160.751 kb on - strand at 160.751 kb on - strand at 160.751 kb on - strand at 160.751 kb on - strand at 160.787 kb on + strand, within ABZR87_RS16965 at 160.787 kb on + strand, within ABZR87_RS16965 at 160.787 kb on + strand, within ABZR87_RS16965 at 160.885 kb on + strand, within ABZR87_RS16965 at 160.946 kb on - strand, within ABZR87_RS16965 at 161.195 kb on - strand, within ABZR87_RS16965 at 161.320 kb on + strand, within ABZR87_RS16965 at 161.321 kb on - strand, within ABZR87_RS16965 at 161.321 kb on - strand, within ABZR87_RS16965 at 161.404 kb on + strand, within ABZR87_RS16965 at 161.449 kb on + strand, within ABZR87_RS16965 at 161.449 kb on + strand, within ABZR87_RS16965 at 161.449 kb on + strand, within ABZR87_RS16965 at 161.449 kb on + strand, within ABZR87_RS16965 at 161.450 kb on - strand, within ABZR87_RS16965 at 161.485 kb on + strand at 161.486 kb on - strand at 161.486 kb on - strand at 161.510 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 158,325 + -0.8 158,325 + -0.7 158,333 + -0.3 158,334 - +0.6 158,334 - +1.2 158,413 + +0.3 158,413 + -0.0 158,414 - +0.2 158,414 - -0.1 158,414 - +0.2 158,414 - -0.3 158,414 - +0.0 158,414 - +0.7 158,435 + -0.4 158,436 - -0.0 158,445 + +0.4 158,445 + -0.5 158,446 - +0.8 158,446 - -0.8 158,446 - -0.6 158,446 - +0.5 158,474 + +0.4 158,474 + -0.1 158,475 - +0.4 158,626 + +0.1 158,627 - +1.7 158,627 - +0.5 158,627 - +0.8 158,627 - -0.1 158,798 + ABZR87_RS16955 0.32 -0.4 158,798 + ABZR87_RS16955 0.32 +0.4 158,799 - ABZR87_RS16955 0.33 -0.1 158,918 + ABZR87_RS16955 0.49 -0.1 158,918 + ABZR87_RS16955 0.49 +0.0 158,919 - ABZR87_RS16955 0.49 +0.2 159,065 - ABZR87_RS16955 0.68 -0.6 159,229 + -0.0 159,229 + -0.1 159,230 - -0.6 159,256 + +0.4 159,256 + -0.1 159,256 + -0.5 159,256 + +0.4 159,256 + +1.6 159,256 + -1.8 159,256 + +0.1 159,256 + +0.8 159,257 - +0.6 159,257 - -1.1 159,257 - -0.4 159,257 - -0.6 159,257 - +0.1 159,390 + -0.9 159,391 - -0.6 159,426 + +3.5 159,426 + -0.1 159,427 - -0.1 159,552 + ABZR87_RS16960 0.18 +0.9 159,553 - ABZR87_RS16960 0.19 -0.1 159,553 - ABZR87_RS16960 0.19 -1.9 159,553 - ABZR87_RS16960 0.19 +0.8 159,558 + ABZR87_RS16960 0.19 -2.4 159,558 + ABZR87_RS16960 0.19 +0.4 159,558 + ABZR87_RS16960 0.19 +0.9 159,559 - ABZR87_RS16960 0.19 -1.1 159,559 - ABZR87_RS16960 0.19 -0.9 159,559 - ABZR87_RS16960 0.19 -0.4 159,559 - ABZR87_RS16960 0.19 +0.4 159,781 - ABZR87_RS16960 0.36 +1.4 159,781 - ABZR87_RS16960 0.36 +1.4 160,149 + ABZR87_RS16960 0.64 +0.5 160,150 - ABZR87_RS16960 0.64 -0.9 160,224 + ABZR87_RS16960 0.69 -0.4 160,224 + ABZR87_RS16960 0.69 -2.3 160,224 + ABZR87_RS16960 0.69 -1.5 160,225 - ABZR87_RS16960 0.70 +0.3 160,731 - +1.1 160,731 - +0.4 160,744 + -0.1 160,744 + +0.4 160,744 + +0.7 160,744 + +0.2 160,750 + +2.2 160,750 + +0.4 160,750 + +0.9 160,750 + -0.4 160,750 + -0.1 160,750 + -1.6 160,750 + +0.0 160,750 + -0.1 160,750 + -0.0 160,750 + +0.0 160,751 - -0.5 160,751 - -0.5 160,751 - +0.2 160,751 - +0.1 160,751 - -0.1 160,751 - -1.1 160,751 - -0.2 160,751 - +0.5 160,751 - +0.1 160,751 - +0.4 160,787 + ABZR87_RS16965 0.14 +0.4 160,787 + ABZR87_RS16965 0.14 -2.6 160,787 + ABZR87_RS16965 0.14 -1.6 160,885 + ABZR87_RS16965 0.25 -0.6 160,946 - ABZR87_RS16965 0.32 +0.4 161,195 - ABZR87_RS16965 0.60 -0.5 161,320 + ABZR87_RS16965 0.74 -0.2 161,321 - ABZR87_RS16965 0.75 +0.0 161,321 - ABZR87_RS16965 0.75 -0.1 161,404 + ABZR87_RS16965 0.84 -0.4 161,449 + ABZR87_RS16965 0.89 +0.9 161,449 + ABZR87_RS16965 0.89 +0.7 161,449 + ABZR87_RS16965 0.89 -0.4 161,449 + ABZR87_RS16965 0.89 -1.4 161,450 - ABZR87_RS16965 0.89 -2.5 161,485 + +0.9 161,486 - +0.5 161,486 - -0.5 161,510 - +0.4
Or see this region's nucleotide sequence