Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS15430 and ABZR87_RS15435 overlap by 4 nucleotides ABZR87_RS15435 and ABZR87_RS15440 are separated by 127 nucleotides ABZR87_RS15440 and ABZR87_RS15445 are separated by 22 nucleotides
ABZR87_RS15430: ABZR87_RS15430 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 3,247,129 to 3,247,599
_RS15430
ABZR87_RS15435: ABZR87_RS15435 - Maf family protein, at 3,247,596 to 3,248,219
_RS15435
ABZR87_RS15440: ABZR87_RS15440 - ribonuclease G, at 3,248,347 to 3,249,816
_RS15440
ABZR87_RS15445: ABZR87_RS15445 - MFS transporter, at 3,249,839 to 3,251,230
_RS15445
Position (kb)
3248
3249
3250 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3247.585 kb on + strand at 3247.585 kb on + strand at 3247.586 kb on - strand at 3247.586 kb on - strand at 3247.595 kb on + strand at 3247.595 kb on + strand at 3247.596 kb on - strand at 3247.596 kb on - strand at 3247.596 kb on - strand at 3247.596 kb on - strand at 3247.629 kb on + strand at 3247.630 kb on - strand at 3247.630 kb on - strand at 3247.765 kb on - strand, within ABZR87_RS15435 at 3247.765 kb on - strand, within ABZR87_RS15435 at 3247.864 kb on + strand, within ABZR87_RS15435 at 3247.865 kb on - strand, within ABZR87_RS15435 at 3247.865 kb on - strand, within ABZR87_RS15435 at 3248.058 kb on + strand, within ABZR87_RS15435 at 3248.058 kb on + strand, within ABZR87_RS15435 at 3248.058 kb on + strand, within ABZR87_RS15435 at 3248.059 kb on - strand, within ABZR87_RS15435 at 3248.135 kb on + strand, within ABZR87_RS15435 at 3248.135 kb on + strand, within ABZR87_RS15435 at 3248.136 kb on - strand, within ABZR87_RS15435 at 3248.148 kb on + strand, within ABZR87_RS15435 at 3248.149 kb on - strand, within ABZR87_RS15435 at 3248.167 kb on + strand at 3248.167 kb on + strand at 3248.167 kb on + strand at 3248.218 kb on - strand at 3248.296 kb on - strand at 3248.296 kb on - strand at 3248.296 kb on - strand at 3248.307 kb on + strand at 3248.308 kb on - strand at 3248.338 kb on + strand at 3248.338 kb on + strand at 3248.339 kb on - strand at 3248.371 kb on - strand at 3248.470 kb on + strand at 3248.470 kb on + strand at 3248.470 kb on + strand at 3248.731 kb on + strand, within ABZR87_RS15440 at 3248.731 kb on + strand, within ABZR87_RS15440 at 3248.732 kb on - strand, within ABZR87_RS15440 at 3248.732 kb on - strand, within ABZR87_RS15440 at 3248.906 kb on - strand, within ABZR87_RS15440 at 3248.972 kb on - strand, within ABZR87_RS15440 at 3249.121 kb on + strand, within ABZR87_RS15440 at 3249.121 kb on + strand, within ABZR87_RS15440 at 3249.121 kb on + strand, within ABZR87_RS15440 at 3249.151 kb on + strand, within ABZR87_RS15440 at 3249.152 kb on - strand, within ABZR87_RS15440 at 3249.214 kb on + strand, within ABZR87_RS15440 at 3249.272 kb on - strand, within ABZR87_RS15440 at 3249.796 kb on + strand at 3249.815 kb on - strand at 3249.815 kb on - strand at 3249.947 kb on + strand at 3249.948 kb on - strand at 3249.948 kb on - strand at 3250.104 kb on + strand, within ABZR87_RS15445 at 3250.104 kb on + strand, within ABZR87_RS15445 at 3250.104 kb on + strand, within ABZR87_RS15445 at 3250.104 kb on + strand, within ABZR87_RS15445 at 3250.105 kb on - strand, within ABZR87_RS15445 at 3250.119 kb on + strand, within ABZR87_RS15445 at 3250.120 kb on - strand, within ABZR87_RS15445 at 3250.120 kb on - strand, within ABZR87_RS15445 at 3250.120 kb on - strand, within ABZR87_RS15445 at 3250.120 kb on - strand, within ABZR87_RS15445 at 3250.327 kb on - strand, within ABZR87_RS15445 at 3250.355 kb on + strand, within ABZR87_RS15445 at 3250.355 kb on + strand, within ABZR87_RS15445 at 3250.355 kb on + strand, within ABZR87_RS15445 at 3250.407 kb on + strand, within ABZR87_RS15445 at 3250.408 kb on - strand, within ABZR87_RS15445 at 3250.408 kb on - strand, within ABZR87_RS15445 at 3250.704 kb on + strand, within ABZR87_RS15445 at 3250.705 kb on - strand, within ABZR87_RS15445
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 3,247,585 + -1.1 3,247,585 + +0.2 3,247,586 - +0.8 3,247,586 - -0.9 3,247,595 + -0.5 3,247,595 + +0.5 3,247,596 - +0.6 3,247,596 - +0.1 3,247,596 - -0.7 3,247,596 - +0.1 3,247,629 + -0.2 3,247,630 - -2.0 3,247,630 - +0.2 3,247,765 - ABZR87_RS15435 0.27 -0.3 3,247,765 - ABZR87_RS15435 0.27 -0.0 3,247,864 + ABZR87_RS15435 0.43 -0.1 3,247,865 - ABZR87_RS15435 0.43 +0.1 3,247,865 - ABZR87_RS15435 0.43 +0.5 3,248,058 + ABZR87_RS15435 0.74 -1.2 3,248,058 + ABZR87_RS15435 0.74 -0.2 3,248,058 + ABZR87_RS15435 0.74 +0.3 3,248,059 - ABZR87_RS15435 0.74 +0.6 3,248,135 + ABZR87_RS15435 0.86 -0.3 3,248,135 + ABZR87_RS15435 0.86 +0.1 3,248,136 - ABZR87_RS15435 0.87 -0.5 3,248,148 + ABZR87_RS15435 0.88 -0.8 3,248,149 - ABZR87_RS15435 0.89 +0.1 3,248,167 + +0.3 3,248,167 + +0.4 3,248,167 + +1.0 3,248,218 - -0.4 3,248,296 - -0.3 3,248,296 - +0.3 3,248,296 - -0.3 3,248,307 + +0.4 3,248,308 - -0.3 3,248,338 + +0.1 3,248,338 + +1.4 3,248,339 - +1.2 3,248,371 - -1.9 3,248,470 + +1.9 3,248,470 + +0.5 3,248,470 + -0.7 3,248,731 + ABZR87_RS15440 0.26 -0.1 3,248,731 + ABZR87_RS15440 0.26 -1.3 3,248,732 - ABZR87_RS15440 0.26 +0.3 3,248,732 - ABZR87_RS15440 0.26 -0.4 3,248,906 - ABZR87_RS15440 0.38 +0.3 3,248,972 - ABZR87_RS15440 0.43 -0.4 3,249,121 + ABZR87_RS15440 0.53 -0.7 3,249,121 + ABZR87_RS15440 0.53 -1.4 3,249,121 + ABZR87_RS15440 0.53 +0.3 3,249,151 + ABZR87_RS15440 0.55 -1.6 3,249,152 - ABZR87_RS15440 0.55 -1.6 3,249,214 + ABZR87_RS15440 0.59 +0.3 3,249,272 - ABZR87_RS15440 0.63 -0.6 3,249,796 + -0.9 3,249,815 - +0.3 3,249,815 - +0.2 3,249,947 + -0.0 3,249,948 - -1.0 3,249,948 - +0.6 3,250,104 + ABZR87_RS15445 0.19 -0.5 3,250,104 + ABZR87_RS15445 0.19 -0.1 3,250,104 + ABZR87_RS15445 0.19 -0.1 3,250,104 + ABZR87_RS15445 0.19 +0.4 3,250,105 - ABZR87_RS15445 0.19 -0.5 3,250,119 + ABZR87_RS15445 0.20 -0.7 3,250,120 - ABZR87_RS15445 0.20 +0.3 3,250,120 - ABZR87_RS15445 0.20 -0.7 3,250,120 - ABZR87_RS15445 0.20 -0.6 3,250,120 - ABZR87_RS15445 0.20 -0.1 3,250,327 - ABZR87_RS15445 0.35 +0.1 3,250,355 + ABZR87_RS15445 0.37 +0.3 3,250,355 + ABZR87_RS15445 0.37 -0.1 3,250,355 + ABZR87_RS15445 0.37 -0.4 3,250,407 + ABZR87_RS15445 0.41 -2.0 3,250,408 - ABZR87_RS15445 0.41 -0.4 3,250,408 - ABZR87_RS15445 0.41 +1.4 3,250,704 + ABZR87_RS15445 0.62 +0.8 3,250,705 - ABZR87_RS15445 0.62 -0.7
Or see this region's nucleotide sequence