Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS07800 and ABZR87_RS07805 are separated by 20 nucleotides ABZR87_RS07805 and ABZR87_RS07810 are separated by 87 nucleotides
ABZR87_RS07800: ABZR87_RS07800 - DUF1571 domain-containing protein, at 1,654,622 to 1,655,539
_RS07800
ABZR87_RS07805: ABZR87_RS07805 - AMP-binding protein, at 1,655,560 to 1,657,299
_RS07805
ABZR87_RS07810: ABZR87_RS07810 - acetyl-CoA C-acetyltransferase, at 1,657,387 to 1,658,706
_RS07810
Position (kb)
1655
1656
1657
1658 Strain fitness (log2 ratio)
-2
-1
0
1 at 1654.569 kb on + strand at 1654.570 kb on - strand at 1654.570 kb on - strand at 1654.623 kb on + strand at 1654.746 kb on - strand, within ABZR87_RS07800 at 1654.770 kb on + strand, within ABZR87_RS07800 at 1654.770 kb on + strand, within ABZR87_RS07800 at 1654.770 kb on + strand, within ABZR87_RS07800 at 1654.771 kb on - strand, within ABZR87_RS07800 at 1654.771 kb on - strand, within ABZR87_RS07800 at 1654.791 kb on + strand, within ABZR87_RS07800 at 1654.791 kb on + strand, within ABZR87_RS07800 at 1654.791 kb on + strand, within ABZR87_RS07800 at 1654.791 kb on + strand, within ABZR87_RS07800 at 1654.792 kb on - strand, within ABZR87_RS07800 at 1654.792 kb on - strand, within ABZR87_RS07800 at 1655.046 kb on + strand, within ABZR87_RS07800 at 1655.046 kb on + strand, within ABZR87_RS07800 at 1655.046 kb on + strand, within ABZR87_RS07800 at 1655.046 kb on + strand, within ABZR87_RS07800 at 1655.046 kb on + strand, within ABZR87_RS07800 at 1655.047 kb on - strand, within ABZR87_RS07800 at 1655.047 kb on - strand, within ABZR87_RS07800 at 1655.047 kb on - strand, within ABZR87_RS07800 at 1655.076 kb on + strand, within ABZR87_RS07800 at 1655.076 kb on + strand, within ABZR87_RS07800 at 1655.125 kb on - strand, within ABZR87_RS07800 at 1655.125 kb on - strand, within ABZR87_RS07800 at 1655.223 kb on + strand, within ABZR87_RS07800 at 1655.223 kb on + strand, within ABZR87_RS07800 at 1655.224 kb on - strand, within ABZR87_RS07800 at 1655.527 kb on + strand at 1655.539 kb on + strand at 1655.540 kb on - strand at 1655.562 kb on - strand at 1655.562 kb on - strand at 1655.690 kb on + strand at 1655.764 kb on - strand, within ABZR87_RS07805 at 1655.921 kb on + strand, within ABZR87_RS07805 at 1655.922 kb on - strand, within ABZR87_RS07805 at 1656.140 kb on + strand, within ABZR87_RS07805 at 1656.195 kb on - strand, within ABZR87_RS07805 at 1656.427 kb on - strand, within ABZR87_RS07805 at 1656.485 kb on + strand, within ABZR87_RS07805 at 1656.626 kb on + strand, within ABZR87_RS07805 at 1656.627 kb on - strand, within ABZR87_RS07805 at 1656.627 kb on - strand, within ABZR87_RS07805 at 1656.951 kb on - strand, within ABZR87_RS07805 at 1656.951 kb on - strand, within ABZR87_RS07805 at 1657.119 kb on - strand, within ABZR87_RS07805 at 1657.119 kb on - strand, within ABZR87_RS07805 at 1657.145 kb on + strand at 1657.163 kb on + strand at 1657.206 kb on - strand at 1657.244 kb on + strand at 1657.244 kb on + strand at 1657.245 kb on - strand at 1657.278 kb on + strand at 1657.338 kb on - strand at 1657.338 kb on - strand at 1657.564 kb on + strand, within ABZR87_RS07810 at 1657.565 kb on - strand, within ABZR87_RS07810 at 1657.565 kb on - strand, within ABZR87_RS07810 at 1657.565 kb on - strand, within ABZR87_RS07810 at 1657.629 kb on - strand, within ABZR87_RS07810 at 1657.629 kb on - strand, within ABZR87_RS07810 at 1657.694 kb on + strand, within ABZR87_RS07810 at 1657.694 kb on + strand, within ABZR87_RS07810 at 1657.695 kb on - strand, within ABZR87_RS07810 at 1657.695 kb on - strand, within ABZR87_RS07810 at 1657.698 kb on - strand, within ABZR87_RS07810 at 1657.751 kb on + strand, within ABZR87_RS07810 at 1657.751 kb on + strand, within ABZR87_RS07810 at 1657.835 kb on + strand, within ABZR87_RS07810 at 1657.836 kb on - strand, within ABZR87_RS07810 at 1657.836 kb on - strand, within ABZR87_RS07810 at 1657.836 kb on - strand, within ABZR87_RS07810 at 1657.836 kb on - strand, within ABZR87_RS07810 at 1657.836 kb on - strand, within ABZR87_RS07810 at 1658.069 kb on + strand, within ABZR87_RS07810
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,654,569 + +0.0 1,654,570 - -0.6 1,654,570 - -0.2 1,654,623 + -0.5 1,654,746 - ABZR87_RS07800 0.14 +0.2 1,654,770 + ABZR87_RS07800 0.16 +0.8 1,654,770 + ABZR87_RS07800 0.16 +0.3 1,654,770 + ABZR87_RS07800 0.16 -0.7 1,654,771 - ABZR87_RS07800 0.16 +0.0 1,654,771 - ABZR87_RS07800 0.16 +1.1 1,654,791 + ABZR87_RS07800 0.18 -0.2 1,654,791 + ABZR87_RS07800 0.18 -1.4 1,654,791 + ABZR87_RS07800 0.18 -0.3 1,654,791 + ABZR87_RS07800 0.18 +0.5 1,654,792 - ABZR87_RS07800 0.19 +1.1 1,654,792 - ABZR87_RS07800 0.19 -0.7 1,655,046 + ABZR87_RS07800 0.46 +0.1 1,655,046 + ABZR87_RS07800 0.46 -0.0 1,655,046 + ABZR87_RS07800 0.46 -0.5 1,655,046 + ABZR87_RS07800 0.46 -0.3 1,655,046 + ABZR87_RS07800 0.46 -0.8 1,655,047 - ABZR87_RS07800 0.46 -0.6 1,655,047 - ABZR87_RS07800 0.46 -0.1 1,655,047 - ABZR87_RS07800 0.46 +0.5 1,655,076 + ABZR87_RS07800 0.49 -0.6 1,655,076 + ABZR87_RS07800 0.49 +0.3 1,655,125 - ABZR87_RS07800 0.55 -0.3 1,655,125 - ABZR87_RS07800 0.55 +1.4 1,655,223 + ABZR87_RS07800 0.65 -1.2 1,655,223 + ABZR87_RS07800 0.65 -1.6 1,655,224 - ABZR87_RS07800 0.66 -0.1 1,655,527 + -0.7 1,655,539 + +0.4 1,655,540 - -0.3 1,655,562 - +0.4 1,655,562 - +0.7 1,655,690 + +0.1 1,655,764 - ABZR87_RS07805 0.12 -0.7 1,655,921 + ABZR87_RS07805 0.21 -0.9 1,655,922 - ABZR87_RS07805 0.21 -0.5 1,656,140 + ABZR87_RS07805 0.33 -1.5 1,656,195 - ABZR87_RS07805 0.36 -2.0 1,656,427 - ABZR87_RS07805 0.50 +0.2 1,656,485 + ABZR87_RS07805 0.53 -0.1 1,656,626 + ABZR87_RS07805 0.61 -1.6 1,656,627 - ABZR87_RS07805 0.61 -1.0 1,656,627 - ABZR87_RS07805 0.61 +0.9 1,656,951 - ABZR87_RS07805 0.80 -0.9 1,656,951 - ABZR87_RS07805 0.80 -0.4 1,657,119 - ABZR87_RS07805 0.90 -2.3 1,657,119 - ABZR87_RS07805 0.90 -0.8 1,657,145 + -0.2 1,657,163 + -0.1 1,657,206 - -0.6 1,657,244 + -0.7 1,657,244 + +0.3 1,657,245 - -0.7 1,657,278 + -0.8 1,657,338 - -1.4 1,657,338 - +0.5 1,657,564 + ABZR87_RS07810 0.13 -0.1 1,657,565 - ABZR87_RS07810 0.13 +0.5 1,657,565 - ABZR87_RS07810 0.13 +0.3 1,657,565 - ABZR87_RS07810 0.13 +0.5 1,657,629 - ABZR87_RS07810 0.18 +0.1 1,657,629 - ABZR87_RS07810 0.18 +0.0 1,657,694 + ABZR87_RS07810 0.23 +0.7 1,657,694 + ABZR87_RS07810 0.23 -0.4 1,657,695 - ABZR87_RS07810 0.23 +0.3 1,657,695 - ABZR87_RS07810 0.23 +0.1 1,657,698 - ABZR87_RS07810 0.24 +0.7 1,657,751 + ABZR87_RS07810 0.28 -0.6 1,657,751 + ABZR87_RS07810 0.28 -2.3 1,657,835 + ABZR87_RS07810 0.34 -0.2 1,657,836 - ABZR87_RS07810 0.34 -0.9 1,657,836 - ABZR87_RS07810 0.34 -1.0 1,657,836 - ABZR87_RS07810 0.34 -0.8 1,657,836 - ABZR87_RS07810 0.34 -0.0 1,657,836 - ABZR87_RS07810 0.34 -0.0 1,658,069 + ABZR87_RS07810 0.52 +0.8
Or see this region's nucleotide sequence