Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS05125 and ABZR87_RS05130 are separated by 97 nucleotides ABZR87_RS05130 and ABZR87_RS05135 are separated by 33 nucleotides ABZR87_RS05135 and ABZR87_RS05140 are separated by 47 nucleotides ABZR87_RS05140 and ABZR87_RS05145 are separated by 142 nucleotides ABZR87_RS05145 and ABZR87_RS05150 are separated by 46 nucleotides ABZR87_RS05150 and ABZR87_RS05155 are separated by 0 nucleotides
ABZR87_RS05125: ABZR87_RS05125 - hypothetical protein, at 1,080,581 to 1,081,195
_RS05125
ABZR87_RS05130: ABZR87_RS05130 - CsbD family protein, at 1,081,293 to 1,081,496
_RS05130
ABZR87_RS05135: ABZR87_RS05135 - response regulator transcription factor, at 1,081,530 to 1,082,108
_RS05135
ABZR87_RS05140: ABZR87_RS05140 - ATP-binding protein, at 1,082,156 to 1,083,502
_RS05140
ABZR87_RS05145: ABZR87_RS05145 - copper chaperone PCu(A)C, at 1,083,645 to 1,084,142
_RS05145
ABZR87_RS05150: ABZR87_RS05150 - hypothetical protein, at 1,084,189 to 1,084,410
_RS05150
ABZR87_RS05155: ABZR87_RS05155 - cysteine-rich CWC family protein, at 1,084,411 to 1,084,593
_RS05155
Position (kb)
1082
1083
1084 Strain fitness (log2 ratio)
-1
0
1
2 at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.193 kb on + strand at 1081.194 kb on - strand at 1081.194 kb on - strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.292 kb on + strand at 1081.293 kb on - strand at 1081.293 kb on - strand at 1081.293 kb on - strand at 1081.438 kb on - strand, within ABZR87_RS05130 at 1081.510 kb on + strand at 1081.510 kb on + strand at 1081.510 kb on + strand at 1081.511 kb on - strand at 1081.511 kb on - strand at 1081.511 kb on - strand at 1081.511 kb on - strand at 1081.531 kb on + strand at 1081.531 kb on + strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.532 kb on - strand at 1081.809 kb on - strand, within ABZR87_RS05135 at 1082.126 kb on + strand at 1082.157 kb on + strand at 1082.157 kb on + strand at 1082.158 kb on - strand at 1082.158 kb on - strand at 1082.158 kb on - strand at 1082.310 kb on + strand, within ABZR87_RS05140 at 1082.483 kb on + strand, within ABZR87_RS05140 at 1083.076 kb on - strand, within ABZR87_RS05140 at 1083.097 kb on - strand, within ABZR87_RS05140 at 1083.272 kb on - strand, within ABZR87_RS05140 at 1083.452 kb on + strand at 1083.452 kb on + strand at 1083.453 kb on - strand at 1083.453 kb on - strand at 1083.515 kb on + strand at 1083.515 kb on + strand at 1083.516 kb on - strand at 1083.584 kb on + strand at 1083.584 kb on + strand at 1083.584 kb on + strand at 1083.584 kb on + strand at 1083.584 kb on + strand at 1083.585 kb on - strand at 1083.585 kb on - strand at 1083.585 kb on - strand at 1083.608 kb on + strand at 1083.609 kb on - strand at 1083.963 kb on + strand, within ABZR87_RS05145 at 1084.174 kb on + strand at 1084.174 kb on + strand at 1084.174 kb on + strand at 1084.174 kb on + strand at 1084.174 kb on + strand at 1084.174 kb on + strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.175 kb on - strand at 1084.194 kb on + strand at 1084.195 kb on - strand at 1084.195 kb on - strand at 1084.195 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,081,193 + +0.2 1,081,193 + -0.4 1,081,193 + -0.2 1,081,193 + +0.4 1,081,193 + +1.0 1,081,193 + +0.9 1,081,194 - +1.0 1,081,194 - +0.2 1,081,292 + -1.7 1,081,292 + +0.9 1,081,292 + -0.3 1,081,292 + +0.1 1,081,292 + -0.1 1,081,292 + -0.4 1,081,292 + -1.4 1,081,292 + -0.8 1,081,292 + -0.7 1,081,292 + -0.0 1,081,293 - +0.2 1,081,293 - -1.7 1,081,293 - -0.7 1,081,438 - ABZR87_RS05130 0.71 -1.3 1,081,510 + +0.6 1,081,510 + +0.2 1,081,510 + -0.6 1,081,511 - +0.8 1,081,511 - -0.5 1,081,511 - -0.3 1,081,511 - +0.9 1,081,531 + +0.1 1,081,531 + -0.2 1,081,532 - +0.6 1,081,532 - +1.6 1,081,532 - -0.5 1,081,532 - +0.2 1,081,532 - +0.1 1,081,532 - +2.6 1,081,809 - ABZR87_RS05135 0.48 -0.4 1,082,126 + -0.4 1,082,157 + +0.1 1,082,157 + -0.2 1,082,158 - -0.0 1,082,158 - +0.6 1,082,158 - -0.1 1,082,310 + ABZR87_RS05140 0.11 -0.4 1,082,483 + ABZR87_RS05140 0.24 -0.4 1,083,076 - ABZR87_RS05140 0.68 -0.7 1,083,097 - ABZR87_RS05140 0.70 +0.6 1,083,272 - ABZR87_RS05140 0.83 -0.0 1,083,452 + -0.6 1,083,452 + -0.5 1,083,453 - -0.7 1,083,453 - +0.6 1,083,515 + -0.2 1,083,515 + -0.2 1,083,516 - -0.1 1,083,584 + +0.1 1,083,584 + +0.1 1,083,584 + +0.0 1,083,584 + +0.3 1,083,584 + -1.5 1,083,585 - +1.4 1,083,585 - -0.2 1,083,585 - -0.8 1,083,608 + -0.4 1,083,609 - +0.1 1,083,963 + ABZR87_RS05145 0.64 -0.9 1,084,174 + -0.6 1,084,174 + +0.2 1,084,174 + -1.7 1,084,174 + -0.7 1,084,174 + -0.7 1,084,174 + +0.2 1,084,175 - -0.3 1,084,175 - -1.2 1,084,175 - -0.1 1,084,175 - -0.0 1,084,175 - -0.5 1,084,175 - -0.9 1,084,175 - +2.3 1,084,175 - -0.5 1,084,175 - -0.5 1,084,175 - +0.8 1,084,175 - -0.4 1,084,194 + -0.2 1,084,195 - -1.0 1,084,195 - -0.7 1,084,195 - -0.4
Or see this region's nucleotide sequence