Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS04970 and ABZR87_RS04975 are separated by 10 nucleotides ABZR87_RS04975 and ABZR87_RS04980 are separated by 252 nucleotides
ABZR87_RS04970: ABZR87_RS04970 - glycosyltransferase family 39 protein, at 1,042,585 to 1,044,609
_RS04970
ABZR87_RS04975: ABZR87_RS04975 - peptide deformylase, at 1,044,620 to 1,045,135
_RS04975
ABZR87_RS04980: ABZR87_RS04980 - LysM peptidoglycan-binding domain-containing protein, at 1,045,388 to 1,046,575
_RS04980
Position (kb)
1044
1045
1046 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1043.753 kb on + strand, within ABZR87_RS04970 at 1043.753 kb on + strand, within ABZR87_RS04970 at 1043.753 kb on + strand, within ABZR87_RS04970 at 1043.753 kb on + strand, within ABZR87_RS04970 at 1043.754 kb on - strand, within ABZR87_RS04970 at 1043.754 kb on - strand, within ABZR87_RS04970 at 1043.754 kb on - strand, within ABZR87_RS04970 at 1043.754 kb on - strand, within ABZR87_RS04970 at 1043.754 kb on - strand, within ABZR87_RS04970 at 1043.754 kb on - strand, within ABZR87_RS04970 at 1043.754 kb on - strand, within ABZR87_RS04970 at 1043.754 kb on - strand, within ABZR87_RS04970 at 1043.957 kb on + strand, within ABZR87_RS04970 at 1043.958 kb on - strand, within ABZR87_RS04970 at 1043.958 kb on - strand, within ABZR87_RS04970 at 1044.065 kb on + strand, within ABZR87_RS04970 at 1044.066 kb on - strand, within ABZR87_RS04970 at 1044.066 kb on - strand, within ABZR87_RS04970 at 1044.066 kb on - strand, within ABZR87_RS04970 at 1044.066 kb on - strand, within ABZR87_RS04970 at 1044.067 kb on - strand, within ABZR87_RS04970 at 1044.256 kb on + strand, within ABZR87_RS04970 at 1044.257 kb on - strand, within ABZR87_RS04970 at 1044.258 kb on - strand, within ABZR87_RS04970 at 1044.314 kb on + strand, within ABZR87_RS04970 at 1044.314 kb on + strand, within ABZR87_RS04970 at 1044.314 kb on + strand, within ABZR87_RS04970 at 1044.314 kb on + strand, within ABZR87_RS04970 at 1044.314 kb on + strand, within ABZR87_RS04970 at 1044.315 kb on - strand, within ABZR87_RS04970 at 1044.315 kb on - strand, within ABZR87_RS04970 at 1044.315 kb on - strand, within ABZR87_RS04970 at 1044.408 kb on - strand at 1044.410 kb on + strand at 1044.410 kb on + strand at 1044.410 kb on + strand at 1044.410 kb on + strand at 1044.410 kb on + strand at 1044.410 kb on + strand at 1044.411 kb on - strand at 1044.411 kb on - strand at 1044.461 kb on + strand at 1044.461 kb on + strand at 1044.461 kb on + strand at 1044.462 kb on - strand at 1044.462 kb on - strand at 1044.532 kb on + strand at 1044.532 kb on + strand at 1044.532 kb on + strand at 1044.533 kb on - strand at 1044.533 kb on - strand at 1044.533 kb on - strand at 1044.533 kb on - strand at 1044.533 kb on - strand at 1044.533 kb on - strand at 1044.696 kb on + strand, within ABZR87_RS04975 at 1044.696 kb on + strand, within ABZR87_RS04975 at 1044.696 kb on + strand, within ABZR87_RS04975 at 1044.697 kb on - strand, within ABZR87_RS04975 at 1044.697 kb on - strand, within ABZR87_RS04975 at 1044.697 kb on - strand, within ABZR87_RS04975 at 1044.697 kb on - strand, within ABZR87_RS04975 at 1045.245 kb on - strand at 1045.245 kb on - strand at 1045.275 kb on + strand at 1045.304 kb on + strand at 1045.304 kb on + strand at 1045.304 kb on + strand at 1045.304 kb on + strand at 1045.304 kb on + strand at 1045.305 kb on - strand at 1045.305 kb on - strand at 1045.360 kb on + strand at 1045.360 kb on + strand at 1045.360 kb on + strand at 1045.361 kb on - strand at 1045.361 kb on - strand at 1045.361 kb on - strand at 1045.365 kb on + strand at 1045.365 kb on + strand at 1045.365 kb on + strand at 1045.365 kb on + strand at 1045.365 kb on + strand at 1045.365 kb on + strand at 1045.366 kb on - strand at 1045.366 kb on - strand at 1045.366 kb on - strand at 1045.366 kb on - strand at 1045.366 kb on - strand at 1045.378 kb on + strand at 1045.379 kb on - strand at 1045.379 kb on - strand at 1045.436 kb on + strand at 1045.437 kb on - strand at 1045.598 kb on + strand, within ABZR87_RS04980 at 1045.598 kb on + strand, within ABZR87_RS04980 at 1045.598 kb on + strand, within ABZR87_RS04980 at 1045.598 kb on + strand, within ABZR87_RS04980 at 1045.598 kb on + strand, within ABZR87_RS04980 at 1045.598 kb on + strand, within ABZR87_RS04980 at 1045.598 kb on + strand, within ABZR87_RS04980 at 1045.598 kb on + strand, within ABZR87_RS04980 at 1045.643 kb on + strand, within ABZR87_RS04980 at 1045.643 kb on + strand, within ABZR87_RS04980 at 1045.643 kb on + strand, within ABZR87_RS04980 at 1045.643 kb on + strand, within ABZR87_RS04980 at 1045.643 kb on + strand, within ABZR87_RS04980 at 1045.643 kb on + strand, within ABZR87_RS04980 at 1045.644 kb on - strand, within ABZR87_RS04980 at 1045.644 kb on - strand, within ABZR87_RS04980 at 1045.644 kb on - strand, within ABZR87_RS04980 at 1045.658 kb on + strand, within ABZR87_RS04980 at 1045.718 kb on + strand, within ABZR87_RS04980 at 1045.718 kb on + strand, within ABZR87_RS04980 at 1045.718 kb on + strand, within ABZR87_RS04980 at 1045.718 kb on + strand, within ABZR87_RS04980 at 1045.719 kb on - strand, within ABZR87_RS04980 at 1045.721 kb on - strand, within ABZR87_RS04980 at 1045.736 kb on + strand, within ABZR87_RS04980 at 1045.736 kb on + strand, within ABZR87_RS04980 at 1045.737 kb on - strand, within ABZR87_RS04980 at 1045.820 kb on - strand, within ABZR87_RS04980 at 1045.965 kb on - strand, within ABZR87_RS04980 at 1045.965 kb on - strand, within ABZR87_RS04980 at 1045.965 kb on - strand, within ABZR87_RS04980 at 1045.988 kb on + strand, within ABZR87_RS04980 at 1045.988 kb on + strand, within ABZR87_RS04980 at 1046.045 kb on + strand, within ABZR87_RS04980 at 1046.046 kb on - strand, within ABZR87_RS04980 at 1046.046 kb on - strand, within ABZR87_RS04980
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,043,753 + ABZR87_RS04970 0.58 +0.4 1,043,753 + ABZR87_RS04970 0.58 -0.7 1,043,753 + ABZR87_RS04970 0.58 -0.8 1,043,753 + ABZR87_RS04970 0.58 -0.3 1,043,754 - ABZR87_RS04970 0.58 +0.1 1,043,754 - ABZR87_RS04970 0.58 -0.8 1,043,754 - ABZR87_RS04970 0.58 -0.8 1,043,754 - ABZR87_RS04970 0.58 -0.9 1,043,754 - ABZR87_RS04970 0.58 -0.5 1,043,754 - ABZR87_RS04970 0.58 +0.1 1,043,754 - ABZR87_RS04970 0.58 -0.1 1,043,754 - ABZR87_RS04970 0.58 -0.6 1,043,957 + ABZR87_RS04970 0.68 -1.2 1,043,958 - ABZR87_RS04970 0.68 -0.6 1,043,958 - ABZR87_RS04970 0.68 -1.0 1,044,065 + ABZR87_RS04970 0.73 +0.1 1,044,066 - ABZR87_RS04970 0.73 -0.8 1,044,066 - ABZR87_RS04970 0.73 +0.8 1,044,066 - ABZR87_RS04970 0.73 +0.4 1,044,066 - ABZR87_RS04970 0.73 +0.4 1,044,067 - ABZR87_RS04970 0.73 -0.1 1,044,256 + ABZR87_RS04970 0.83 -0.5 1,044,257 - ABZR87_RS04970 0.83 -0.2 1,044,258 - ABZR87_RS04970 0.83 -1.5 1,044,314 + ABZR87_RS04970 0.85 +0.1 1,044,314 + ABZR87_RS04970 0.85 -3.1 1,044,314 + ABZR87_RS04970 0.85 -1.8 1,044,314 + ABZR87_RS04970 0.85 -0.8 1,044,314 + ABZR87_RS04970 0.85 +0.7 1,044,315 - ABZR87_RS04970 0.85 -1.9 1,044,315 - ABZR87_RS04970 0.85 -0.2 1,044,315 - ABZR87_RS04970 0.85 -1.2 1,044,408 - -0.1 1,044,410 + -0.5 1,044,410 + -0.1 1,044,410 + -0.2 1,044,410 + -2.2 1,044,410 + +1.1 1,044,410 + -0.2 1,044,411 - +2.2 1,044,411 - +0.2 1,044,461 + -1.7 1,044,461 + -0.0 1,044,461 + +0.7 1,044,462 - -0.7 1,044,462 - +0.6 1,044,532 + -0.1 1,044,532 + -0.9 1,044,532 + -0.0 1,044,533 - -0.7 1,044,533 - +0.4 1,044,533 - -0.7 1,044,533 - -0.2 1,044,533 - +0.6 1,044,533 - +0.1 1,044,696 + ABZR87_RS04975 0.15 -1.4 1,044,696 + ABZR87_RS04975 0.15 -0.2 1,044,696 + ABZR87_RS04975 0.15 +0.1 1,044,697 - ABZR87_RS04975 0.15 -3.3 1,044,697 - ABZR87_RS04975 0.15 +0.4 1,044,697 - ABZR87_RS04975 0.15 -3.4 1,044,697 - ABZR87_RS04975 0.15 -1.4 1,045,245 - +1.6 1,045,245 - +1.9 1,045,275 + -0.1 1,045,304 + -0.9 1,045,304 + -0.0 1,045,304 + -0.9 1,045,304 + -1.7 1,045,304 + -0.2 1,045,305 - -1.2 1,045,305 - -0.8 1,045,360 + -1.5 1,045,360 + -0.4 1,045,360 + -2.1 1,045,361 - -0.8 1,045,361 - +0.8 1,045,361 - +0.2 1,045,365 + +0.1 1,045,365 + -0.9 1,045,365 + +0.9 1,045,365 + +1.2 1,045,365 + -1.1 1,045,365 + -1.4 1,045,366 - -0.5 1,045,366 - -0.2 1,045,366 - +0.3 1,045,366 - -0.6 1,045,366 - -0.3 1,045,378 + -1.6 1,045,379 - +0.8 1,045,379 - -0.5 1,045,436 + +0.7 1,045,437 - -0.6 1,045,598 + ABZR87_RS04980 0.18 +0.6 1,045,598 + ABZR87_RS04980 0.18 -1.2 1,045,598 + ABZR87_RS04980 0.18 -0.3 1,045,598 + ABZR87_RS04980 0.18 -0.2 1,045,598 + ABZR87_RS04980 0.18 +0.0 1,045,598 + ABZR87_RS04980 0.18 -0.6 1,045,598 + ABZR87_RS04980 0.18 +0.2 1,045,598 + ABZR87_RS04980 0.18 +0.3 1,045,643 + ABZR87_RS04980 0.21 +0.9 1,045,643 + ABZR87_RS04980 0.21 +0.1 1,045,643 + ABZR87_RS04980 0.21 +0.2 1,045,643 + ABZR87_RS04980 0.21 +0.4 1,045,643 + ABZR87_RS04980 0.21 -0.3 1,045,643 + ABZR87_RS04980 0.21 -0.7 1,045,644 - ABZR87_RS04980 0.22 -0.6 1,045,644 - ABZR87_RS04980 0.22 +0.2 1,045,644 - ABZR87_RS04980 0.22 -0.9 1,045,658 + ABZR87_RS04980 0.23 -1.7 1,045,718 + ABZR87_RS04980 0.28 -0.6 1,045,718 + ABZR87_RS04980 0.28 +0.6 1,045,718 + ABZR87_RS04980 0.28 +0.3 1,045,718 + ABZR87_RS04980 0.28 +0.6 1,045,719 - ABZR87_RS04980 0.28 -1.2 1,045,721 - ABZR87_RS04980 0.28 +1.8 1,045,736 + ABZR87_RS04980 0.29 +0.3 1,045,736 + ABZR87_RS04980 0.29 -0.2 1,045,737 - ABZR87_RS04980 0.29 -0.2 1,045,820 - ABZR87_RS04980 0.36 +0.4 1,045,965 - ABZR87_RS04980 0.49 -0.7 1,045,965 - ABZR87_RS04980 0.49 +0.0 1,045,965 - ABZR87_RS04980 0.49 +1.3 1,045,988 + ABZR87_RS04980 0.51 +1.0 1,045,988 + ABZR87_RS04980 0.51 -0.3 1,046,045 + ABZR87_RS04980 0.55 +1.0 1,046,046 - ABZR87_RS04980 0.55 -0.8 1,046,046 - ABZR87_RS04980 0.55 -0.7
Or see this region's nucleotide sequence