Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0544 and DVU0545 overlap by 1 nucleotides DVU0545 and DVU0547 are separated by 448 nucleotides DVU0547 and livH are separated by 103 nucleotides
DVU0544: DVU0544 - hypothetical protein (TIGR), at 615,721 to 616,122
DVU0544
DVU0545: DVU0545 - conserved hypothetical protein (TIGR), at 616,122 to 616,547
DVU0545
DVU0547: DVU0547 - high-affinity branched chain amino acid ABC transporter, periplasmic branched chain amino acid-binding protein (TIGR), at 616,996 to 618,114
DVU0547
DVU0548: livH - high-affinity branched-chain amino acid ABC transporter, permease protein (TIGR), at 618,218 to 619,126
livH
Position (kb)
616
617
618
619 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 616.017 kb on + strand, within DVU0544 at 616.086 kb on + strand at 616.133 kb on - strand at 616.178 kb on - strand, within DVU0545 at 616.193 kb on - strand, within DVU0545 at 616.258 kb on + strand, within DVU0545 at 616.343 kb on + strand, within DVU0545 at 616.353 kb on - strand, within DVU0545 at 616.374 kb on + strand, within DVU0545 at 616.388 kb on - strand, within DVU0545 at 616.471 kb on + strand, within DVU0545 at 616.471 kb on + strand, within DVU0545 at 616.479 kb on - strand, within DVU0545 at 616.492 kb on - strand, within DVU0545 at 616.515 kb on + strand at 616.548 kb on + strand at 616.554 kb on - strand at 616.652 kb on - strand at 616.696 kb on - strand at 616.698 kb on + strand at 616.719 kb on - strand at 616.900 kb on + strand at 617.009 kb on + strand at 617.052 kb on - strand at 617.053 kb on + strand at 617.085 kb on + strand at 617.113 kb on - strand, within DVU0547 at 617.120 kb on + strand, within DVU0547 at 617.125 kb on + strand, within DVU0547 at 617.174 kb on - strand, within DVU0547 at 617.226 kb on + strand, within DVU0547 at 617.234 kb on - strand, within DVU0547 at 617.301 kb on - strand, within DVU0547 at 617.313 kb on + strand, within DVU0547 at 617.386 kb on - strand, within DVU0547 at 617.405 kb on + strand, within DVU0547 at 617.529 kb on + strand, within DVU0547 at 617.537 kb on - strand, within DVU0547 at 617.582 kb on - strand, within DVU0547 at 617.589 kb on + strand, within DVU0547 at 617.609 kb on + strand, within DVU0547 at 617.619 kb on + strand, within DVU0547 at 617.665 kb on + strand, within DVU0547 at 617.687 kb on - strand, within DVU0547 at 617.796 kb on + strand, within DVU0547 at 617.835 kb on - strand, within DVU0547 at 617.868 kb on + strand, within DVU0547 at 617.893 kb on - strand, within DVU0547 at 617.893 kb on - strand, within DVU0547 at 618.035 kb on - strand at 618.059 kb on + strand at 618.136 kb on - strand at 618.292 kb on + strand at 618.292 kb on + strand at 618.297 kb on + strand at 618.333 kb on - strand, within livH at 618.449 kb on + strand, within livH at 618.464 kb on + strand, within livH at 618.464 kb on + strand, within livH at 618.501 kb on - strand, within livH at 618.521 kb on - strand, within livH at 618.588 kb on - strand, within livH at 618.699 kb on + strand, within livH at 618.706 kb on + strand, within livH at 618.716 kb on - strand, within livH at 618.722 kb on + strand, within livH at 618.739 kb on + strand, within livH at 618.744 kb on + strand, within livH at 618.747 kb on - strand, within livH at 618.747 kb on - strand, within livH at 618.747 kb on - strand, within livH at 618.777 kb on + strand, within livH at 618.801 kb on + strand, within livH at 618.815 kb on - strand, within livH at 618.924 kb on - strand, within livH at 619.060 kb on + strand at 619.113 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 616,017 + DVU0544 0.74 -0.5 616,086 + +1.6 616,133 - +0.6 616,178 - DVU0545 0.13 +0.8 616,193 - DVU0545 0.17 -0.9 616,258 + DVU0545 0.32 -0.4 616,343 + DVU0545 0.52 +1.3 616,353 - DVU0545 0.54 -0.1 616,374 + DVU0545 0.59 +0.2 616,388 - DVU0545 0.62 -0.5 616,471 + DVU0545 0.82 -1.0 616,471 + DVU0545 0.82 -0.6 616,479 - DVU0545 0.84 +0.3 616,492 - DVU0545 0.87 -0.1 616,515 + -0.3 616,548 + -0.4 616,554 - -0.1 616,652 - -1.8 616,696 - +0.6 616,698 + -1.3 616,719 - -0.2 616,900 + +0.2 617,009 + -1.0 617,052 - -0.6 617,053 + +0.2 617,085 + -0.5 617,113 - DVU0547 0.10 +0.1 617,120 + DVU0547 0.11 +0.1 617,125 + DVU0547 0.12 -0.0 617,174 - DVU0547 0.16 -0.6 617,226 + DVU0547 0.21 -0.9 617,234 - DVU0547 0.21 +1.0 617,301 - DVU0547 0.27 +0.6 617,313 + DVU0547 0.28 -0.2 617,386 - DVU0547 0.35 +0.1 617,405 + DVU0547 0.37 -0.4 617,529 + DVU0547 0.48 -0.3 617,537 - DVU0547 0.48 -0.1 617,582 - DVU0547 0.52 +0.2 617,589 + DVU0547 0.53 -0.8 617,609 + DVU0547 0.55 -0.6 617,619 + DVU0547 0.56 -0.8 617,665 + DVU0547 0.60 +0.2 617,687 - DVU0547 0.62 +1.1 617,796 + DVU0547 0.71 +0.0 617,835 - DVU0547 0.75 -0.3 617,868 + DVU0547 0.78 +1.5 617,893 - DVU0547 0.80 +0.9 617,893 - DVU0547 0.80 -0.2 618,035 - -0.5 618,059 + -0.1 618,136 - -1.2 618,292 + +1.6 618,292 + +0.2 618,297 + +2.6 618,333 - livH DVU0548 0.13 -0.3 618,449 + livH DVU0548 0.25 +0.3 618,464 + livH DVU0548 0.27 -0.2 618,464 + livH DVU0548 0.27 -0.7 618,501 - livH DVU0548 0.31 -0.1 618,521 - livH DVU0548 0.33 +0.1 618,588 - livH DVU0548 0.41 +0.6 618,699 + livH DVU0548 0.53 -0.7 618,706 + livH DVU0548 0.54 +1.8 618,716 - livH DVU0548 0.55 -0.7 618,722 + livH DVU0548 0.55 -0.5 618,739 + livH DVU0548 0.57 -1.1 618,744 + livH DVU0548 0.58 -1.1 618,747 - livH DVU0548 0.58 -0.9 618,747 - livH DVU0548 0.58 +0.8 618,747 - livH DVU0548 0.58 -0.4 618,777 + livH DVU0548 0.61 -0.3 618,801 + livH DVU0548 0.64 -1.3 618,815 - livH DVU0548 0.66 -0.7 618,924 - livH DVU0548 0.78 +1.6 619,060 + -0.2 619,113 - -0.8
Or see this region's nucleotide sequence