Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt maF and pgpA overlap by 8 nucleotides pgpA and rrf2 are separated by 100 nucleotides rrf2 and rrf1 are separated by 3 nucleotides rrf1 and hmcF are separated by 92 nucleotides
DVU0527: maF - septum formation protein Maf (TIGR), at 600,433 to 601,065
maF
DVU0528: pgpA - phosphatidylglycerophosphatase (TIGR), at 601,058 to 601,537
pgpA
DVU0529: rrf2 - Rrf2 protein (voordouw), at 601,638 to 602,090
rrf2
DVU0530: rrf1 - Rrf1 protein (voordouw), at 602,094 to 602,510
rrf1
DVU0531: hmcF - HmcF, 52.7 kd protein in hmc operon (voordouw), at 602,603 to 603,988
hmcF
Position (kb) 601
602
603 Strain fitness (log2 ratio) -1
0
1
2
3 at 600.642 kb on + strand, within maF at 600.648 kb on - strand, within maF at 600.677 kb on - strand, within maF at 600.692 kb on + strand, within maF at 600.726 kb on + strand, within maF at 600.775 kb on + strand, within maF at 600.822 kb on - strand, within maF at 600.932 kb on - strand, within maF at 601.521 kb on + strand at 601.580 kb on - strand at 601.629 kb on + strand at 601.636 kb on + strand at 601.776 kb on + strand, within rrf2 at 601.791 kb on - strand, within rrf2 at 601.851 kb on + strand, within rrf2 at 601.872 kb on + strand, within rrf2 at 601.872 kb on + strand, within rrf2 at 601.936 kb on + strand, within rrf2 at 602.011 kb on + strand, within rrf2 at 602.021 kb on + strand, within rrf2 at 602.021 kb on + strand, within rrf2 at 602.021 kb on + strand, within rrf2 at 602.085 kb on + strand at 602.087 kb on - strand at 602.143 kb on - strand, within rrf1 at 602.200 kb on - strand, within rrf1 at 602.259 kb on + strand, within rrf1 at 602.353 kb on - strand, within rrf1 at 602.398 kb on - strand, within rrf1 at 602.425 kb on + strand, within rrf1 at 602.440 kb on + strand, within rrf1 at 602.448 kb on - strand, within rrf1 at 602.508 kb on + strand at 602.518 kb on - strand at 602.524 kb on - strand at 602.624 kb on - strand at 602.726 kb on + strand at 602.804 kb on - strand, within hmcF at 603.048 kb on + strand, within hmcF at 603.048 kb on + strand, within hmcF at 603.090 kb on - strand, within hmcF
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 600,642 + maF DVU0527 0.33 -0.2 600,648 - maF DVU0527 0.34 -0.1 600,677 - maF DVU0527 0.39 -0.3 600,692 + maF DVU0527 0.41 -0.5 600,726 + maF DVU0527 0.46 -0.6 600,775 + maF DVU0527 0.54 +0.6 600,822 - maF DVU0527 0.61 +2.1 600,932 - maF DVU0527 0.79 -0.5 601,521 + -1.0 601,580 - -0.4 601,629 + -0.8 601,636 + +0.2 601,776 + rrf2 DVU0529 0.30 +2.6 601,791 - rrf2 DVU0529 0.34 -0.1 601,851 + rrf2 DVU0529 0.47 +0.3 601,872 + rrf2 DVU0529 0.52 +1.3 601,872 + rrf2 DVU0529 0.52 +0.5 601,936 + rrf2 DVU0529 0.66 -0.2 602,011 + rrf2 DVU0529 0.82 -0.4 602,021 + rrf2 DVU0529 0.85 -0.5 602,021 + rrf2 DVU0529 0.85 +0.4 602,021 + rrf2 DVU0529 0.85 +1.0 602,085 + -0.3 602,087 - -1.0 602,143 - rrf1 DVU0530 0.12 +0.6 602,200 - rrf1 DVU0530 0.25 -1.7 602,259 + rrf1 DVU0530 0.40 -1.4 602,353 - rrf1 DVU0530 0.62 -0.0 602,398 - rrf1 DVU0530 0.73 -0.3 602,425 + rrf1 DVU0530 0.79 +1.9 602,440 + rrf1 DVU0530 0.83 +3.6 602,448 - rrf1 DVU0530 0.85 +0.7 602,508 + -0.3 602,518 - -0.7 602,524 - +0.7 602,624 - -0.3 602,726 + +0.0 602,804 - hmcF DVU0531 0.15 +1.0 603,048 + hmcF DVU0531 0.32 -0.0 603,048 + hmcF DVU0531 0.32 -0.2 603,090 - hmcF DVU0531 0.35 -0.0
Or see this region's nucleotide sequence