Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2227 and DVU2228 are separated by 4 nucleotides DVU2228 and motA-2 overlap by 17 nucleotides motA-2 and deoD are separated by 115 nucleotides
DVU2227: DVU2227 - hypothetical protein (TIGR), at 2,320,293 to 2,320,736
DVU2227
DVU2228: DVU2228 - motB protein, putative (TIGR), at 2,320,741 to 2,321,475
DVU2228
DVU2229: motA-2 - chemotaxis protein MotA (TIGR), at 2,321,459 to 2,322,220
motA-2
DVU2230: deoD - purine nucleoside phosphorylase (TIGR), at 2,322,336 to 2,323,160
deoD
Position (kb)
2321
2322
2323 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2320.534 kb on + strand, within DVU2227 at 2320.537 kb on - strand, within DVU2227 at 2320.570 kb on + strand, within DVU2227 at 2320.588 kb on - strand, within DVU2227 at 2320.663 kb on + strand, within DVU2227 at 2320.663 kb on + strand, within DVU2227 at 2320.673 kb on + strand, within DVU2227 at 2320.673 kb on + strand, within DVU2227 at 2320.673 kb on + strand, within DVU2227 at 2320.673 kb on + strand, within DVU2227 at 2320.689 kb on - strand, within DVU2227 at 2320.769 kb on + strand at 2320.769 kb on + strand at 2320.800 kb on + strand at 2320.939 kb on - strand, within DVU2228 at 2320.954 kb on + strand, within DVU2228 at 2321.044 kb on - strand, within DVU2228 at 2321.058 kb on + strand, within DVU2228 at 2321.093 kb on - strand, within DVU2228 at 2321.120 kb on - strand, within DVU2228 at 2321.152 kb on + strand, within DVU2228 at 2321.228 kb on + strand, within DVU2228 at 2321.236 kb on - strand, within DVU2228 at 2321.236 kb on - strand, within DVU2228 at 2321.529 kb on + strand at 2321.576 kb on - strand, within motA-2 at 2321.587 kb on - strand, within motA-2 at 2321.604 kb on + strand, within motA-2 at 2321.612 kb on - strand, within motA-2 at 2321.613 kb on - strand, within motA-2 at 2321.663 kb on - strand, within motA-2 at 2321.681 kb on + strand, within motA-2 at 2321.692 kb on + strand, within motA-2 at 2321.901 kb on + strand, within motA-2 at 2321.980 kb on + strand, within motA-2 at 2321.988 kb on - strand, within motA-2 at 2322.002 kb on + strand, within motA-2 at 2322.009 kb on + strand, within motA-2 at 2322.037 kb on - strand, within motA-2 at 2322.051 kb on + strand, within motA-2 at 2322.059 kb on - strand, within motA-2 at 2322.061 kb on + strand, within motA-2 at 2322.082 kb on + strand, within motA-2 at 2322.301 kb on + strand at 2322.306 kb on + strand at 2322.387 kb on - strand at 2322.432 kb on + strand, within deoD at 2322.432 kb on + strand, within deoD at 2322.440 kb on - strand, within deoD at 2322.441 kb on + strand, within deoD at 2322.485 kb on - strand, within deoD at 2322.620 kb on + strand, within deoD at 2322.653 kb on - strand, within deoD at 2322.722 kb on + strand, within deoD at 2322.722 kb on + strand, within deoD at 2322.759 kb on + strand, within deoD at 2322.806 kb on + strand, within deoD at 2322.816 kb on + strand, within deoD at 2322.829 kb on + strand, within deoD at 2322.837 kb on - strand, within deoD at 2322.837 kb on - strand, within deoD at 2322.886 kb on + strand, within deoD at 2322.949 kb on - strand, within deoD at 2323.012 kb on + strand, within deoD at 2323.020 kb on - strand, within deoD at 2323.071 kb on - strand, within deoD at 2323.086 kb on - strand at 2323.096 kb on - strand at 2323.124 kb on + strand at 2323.209 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 2,320,534 + DVU2227 0.54 +0.1 2,320,537 - DVU2227 0.55 +0.1 2,320,570 + DVU2227 0.62 -1.3 2,320,588 - DVU2227 0.66 +0.5 2,320,663 + DVU2227 0.83 +0.1 2,320,663 + DVU2227 0.83 -0.2 2,320,673 + DVU2227 0.86 -0.2 2,320,673 + DVU2227 0.86 -0.0 2,320,673 + DVU2227 0.86 -1.9 2,320,673 + DVU2227 0.86 -0.1 2,320,689 - DVU2227 0.89 -0.1 2,320,769 + +0.4 2,320,769 + +0.0 2,320,800 + -0.3 2,320,939 - DVU2228 0.27 -0.4 2,320,954 + DVU2228 0.29 +0.1 2,321,044 - DVU2228 0.41 -1.0 2,321,058 + DVU2228 0.43 +0.4 2,321,093 - DVU2228 0.48 -0.7 2,321,120 - DVU2228 0.52 -0.5 2,321,152 + DVU2228 0.56 -0.0 2,321,228 + DVU2228 0.66 +0.7 2,321,236 - DVU2228 0.67 +0.3 2,321,236 - DVU2228 0.67 -0.5 2,321,529 + -0.0 2,321,576 - motA-2 DVU2229 0.15 -1.5 2,321,587 - motA-2 DVU2229 0.17 +0.7 2,321,604 + motA-2 DVU2229 0.19 +0.0 2,321,612 - motA-2 DVU2229 0.20 +1.2 2,321,613 - motA-2 DVU2229 0.20 +0.8 2,321,663 - motA-2 DVU2229 0.27 -0.3 2,321,681 + motA-2 DVU2229 0.29 +1.6 2,321,692 + motA-2 DVU2229 0.31 -2.1 2,321,901 + motA-2 DVU2229 0.58 +0.3 2,321,980 + motA-2 DVU2229 0.68 +0.8 2,321,988 - motA-2 DVU2229 0.69 +2.0 2,322,002 + motA-2 DVU2229 0.71 -0.1 2,322,009 + motA-2 DVU2229 0.72 -2.3 2,322,037 - motA-2 DVU2229 0.76 +0.4 2,322,051 + motA-2 DVU2229 0.78 -0.3 2,322,059 - motA-2 DVU2229 0.79 +0.2 2,322,061 + motA-2 DVU2229 0.79 +0.1 2,322,082 + motA-2 DVU2229 0.82 -0.3 2,322,301 + -0.8 2,322,306 + -0.7 2,322,387 - +0.6 2,322,432 + deoD DVU2230 0.12 -0.1 2,322,432 + deoD DVU2230 0.12 +0.8 2,322,440 - deoD DVU2230 0.13 -0.3 2,322,441 + deoD DVU2230 0.13 -0.1 2,322,485 - deoD DVU2230 0.18 +0.1 2,322,620 + deoD DVU2230 0.34 -1.1 2,322,653 - deoD DVU2230 0.38 -0.4 2,322,722 + deoD DVU2230 0.47 -0.2 2,322,722 + deoD DVU2230 0.47 +1.0 2,322,759 + deoD DVU2230 0.51 +1.7 2,322,806 + deoD DVU2230 0.57 -1.2 2,322,816 + deoD DVU2230 0.58 -0.7 2,322,829 + deoD DVU2230 0.60 -0.4 2,322,837 - deoD DVU2230 0.61 -0.3 2,322,837 - deoD DVU2230 0.61 +0.3 2,322,886 + deoD DVU2230 0.67 -0.4 2,322,949 - deoD DVU2230 0.74 -0.1 2,323,012 + deoD DVU2230 0.82 +0.6 2,323,020 - deoD DVU2230 0.83 +0.0 2,323,071 - deoD DVU2230 0.89 +0.2 2,323,086 - +1.0 2,323,096 - -0.0 2,323,124 + +1.6 2,323,209 + -0.6
Or see this region's nucleotide sequence